Wild Sicilian Rosemary: Phytochemical and Morphological Screening and Antioxidant Activity Evaluation of Extracts and Essential Oils

Mario Licata, Raffaele Leone, Claudio Leto, Salvatore La Bella, Teresa Tuttolomondo, Giuseppe Virga, Claudio Leto, Edoardo M. Napoli, Antonella Saija, Antonio Speciale, Laura Siracusa, Laura Rubino, Domenico Trombetta, Giuseppe Ruberto

Risultato della ricerca: Article

17 Citazioni (Scopus)

Abstract

To identify the best biotypes, an extensive survey of Sicilian wild rosemary was carried out bycollecting 57 samples from various sites, followed by taxonomic characterization from an agronomicperspective. All the biotypes collected were classified as Rosmarinus officinalis L. A cluster analysisbased on the morphological characteristics of the plants allowed the division of the biotypes into sevenmain groups, although the characteristics examined were found to be highly similar and not areadependent.Moreover, all samples were analyzed for their phytochemical content, applying an extractionprotocol to obtain the nonvolatile components and hydrodistillation to collect the essential oils for thevolatile components. The extracts were characterized by LC-UV-DAD/ESI-MS, and the essential oils byGC-FID and GC/MS analyses. In the nonvolatile fractions, 18 components were identified, namely, 13flavones, two organic acids, and three diterpenes. In the volatile fractions, a total of 82 components werefound, with as predominant components a-pinene and camphene among the monoterpene hydrocarbonsand 1,8-cineole, camphor, borneol, and verbenone among the oxygenated monoterpenes. Clusteranalyses were carried out on both phytochemical profiles, allowing the separation of the rosemarysamples into different chemical groups. Finally, the total phenol content and the antioxidant activity ofthe essential oils and extracts were determined with the Folin–Ciocalteu (FC) colorimetric assay, the UVradiation-induced peroxidation in liposomal membranes (UV-IP test), and the scavenging activity of thesuperoxide radical (O2 ). The present study confirmed that the essential oils and organic extracts of theSicilian rosemary samples analyzed showed a considerable antioxidant/free radical-scavenging activity.
Lingua originaleEnglish
pagine (da-a)1075-1093
Numero di pagine19
RivistaCHEMISTRY & BIODIVERSITY
Volume12
Stato di pubblicazionePublished - 2015

Fingerprint

Ledum
Essential oils
Phytochemicals
Volatile Oils
Antioxidants
Screening
Monoterpenes
Scavenging
Camphene
Rosmarinus
Camphor
Diterpenes
Organic acids
Phenol
Free radicals
Phenols
Free Radicals
Assays
Membranes
Acids

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biochemistry
  • Chemistry(all)
  • Molecular Medicine
  • Molecular Biology

Cita questo

Wild Sicilian Rosemary: Phytochemical and Morphological Screening and Antioxidant Activity Evaluation of Extracts and Essential Oils. / Licata, Mario; Leone, Raffaele; Leto, Claudio; La Bella, Salvatore; Tuttolomondo, Teresa; Virga, Giuseppe; Leto, Claudio; Napoli, Edoardo M.; Saija, Antonella; Speciale, Antonio; Siracusa, Laura; Rubino, Laura; Trombetta, Domenico; Ruberto, Giuseppe.

In: CHEMISTRY & BIODIVERSITY, Vol. 12, 2015, pag. 1075-1093.

Risultato della ricerca: Article

Licata, Mario ; Leone, Raffaele ; Leto, Claudio ; La Bella, Salvatore ; Tuttolomondo, Teresa ; Virga, Giuseppe ; Leto, Claudio ; Napoli, Edoardo M. ; Saija, Antonella ; Speciale, Antonio ; Siracusa, Laura ; Rubino, Laura ; Trombetta, Domenico ; Ruberto, Giuseppe. / Wild Sicilian Rosemary: Phytochemical and Morphological Screening and Antioxidant Activity Evaluation of Extracts and Essential Oils. In: CHEMISTRY & BIODIVERSITY. 2015 ; Vol. 12. pagg. 1075-1093.
@article{35a9e4f53a4f44f385922ac4250623f1,
title = "Wild Sicilian Rosemary: Phytochemical and Morphological Screening and Antioxidant Activity Evaluation of Extracts and Essential Oils",
abstract = "To identify the best biotypes, an extensive survey of Sicilian wild rosemary was carried out bycollecting 57 samples from various sites, followed by taxonomic characterization from an agronomicperspective. All the biotypes collected were classified as Rosmarinus officinalis L. A cluster analysisbased on the morphological characteristics of the plants allowed the division of the biotypes into sevenmain groups, although the characteristics examined were found to be highly similar and not areadependent.Moreover, all samples were analyzed for their phytochemical content, applying an extractionprotocol to obtain the nonvolatile components and hydrodistillation to collect the essential oils for thevolatile components. The extracts were characterized by LC-UV-DAD/ESI-MS, and the essential oils byGC-FID and GC/MS analyses. In the nonvolatile fractions, 18 components were identified, namely, 13flavones, two organic acids, and three diterpenes. In the volatile fractions, a total of 82 components werefound, with as predominant components a-pinene and camphene among the monoterpene hydrocarbonsand 1,8-cineole, camphor, borneol, and verbenone among the oxygenated monoterpenes. Clusteranalyses were carried out on both phytochemical profiles, allowing the separation of the rosemarysamples into different chemical groups. Finally, the total phenol content and the antioxidant activity ofthe essential oils and extracts were determined with the Folin–Ciocalteu (FC) colorimetric assay, the UVradiation-induced peroxidation in liposomal membranes (UV-IP test), and the scavenging activity of thesuperoxide radical (O2 ). The present study confirmed that the essential oils and organic extracts of theSicilian rosemary samples analyzed showed a considerable antioxidant/free radical-scavenging activity.",
author = "Mario Licata and Raffaele Leone and Claudio Leto and {La Bella}, Salvatore and Teresa Tuttolomondo and Giuseppe Virga and Claudio Leto and Napoli, {Edoardo M.} and Antonella Saija and Antonio Speciale and Laura Siracusa and Laura Rubino and Domenico Trombetta and Giuseppe Ruberto",
year = "2015",
language = "English",
volume = "12",
pages = "1075--1093",
journal = "CHEMISTRY & BIODIVERSITY",
issn = "1612-1872",

}

TY - JOUR

T1 - Wild Sicilian Rosemary: Phytochemical and Morphological Screening and Antioxidant Activity Evaluation of Extracts and Essential Oils

AU - Licata, Mario

AU - Leone, Raffaele

AU - Leto, Claudio

AU - La Bella, Salvatore

AU - Tuttolomondo, Teresa

AU - Virga, Giuseppe

AU - Leto, Claudio

AU - Napoli, Edoardo M.

AU - Saija, Antonella

AU - Speciale, Antonio

AU - Siracusa, Laura

AU - Rubino, Laura

AU - Trombetta, Domenico

AU - Ruberto, Giuseppe

PY - 2015

Y1 - 2015

N2 - To identify the best biotypes, an extensive survey of Sicilian wild rosemary was carried out bycollecting 57 samples from various sites, followed by taxonomic characterization from an agronomicperspective. All the biotypes collected were classified as Rosmarinus officinalis L. A cluster analysisbased on the morphological characteristics of the plants allowed the division of the biotypes into sevenmain groups, although the characteristics examined were found to be highly similar and not areadependent.Moreover, all samples were analyzed for their phytochemical content, applying an extractionprotocol to obtain the nonvolatile components and hydrodistillation to collect the essential oils for thevolatile components. The extracts were characterized by LC-UV-DAD/ESI-MS, and the essential oils byGC-FID and GC/MS analyses. In the nonvolatile fractions, 18 components were identified, namely, 13flavones, two organic acids, and three diterpenes. In the volatile fractions, a total of 82 components werefound, with as predominant components a-pinene and camphene among the monoterpene hydrocarbonsand 1,8-cineole, camphor, borneol, and verbenone among the oxygenated monoterpenes. Clusteranalyses were carried out on both phytochemical profiles, allowing the separation of the rosemarysamples into different chemical groups. Finally, the total phenol content and the antioxidant activity ofthe essential oils and extracts were determined with the Folin–Ciocalteu (FC) colorimetric assay, the UVradiation-induced peroxidation in liposomal membranes (UV-IP test), and the scavenging activity of thesuperoxide radical (O2 ). The present study confirmed that the essential oils and organic extracts of theSicilian rosemary samples analyzed showed a considerable antioxidant/free radical-scavenging activity.

AB - To identify the best biotypes, an extensive survey of Sicilian wild rosemary was carried out bycollecting 57 samples from various sites, followed by taxonomic characterization from an agronomicperspective. All the biotypes collected were classified as Rosmarinus officinalis L. A cluster analysisbased on the morphological characteristics of the plants allowed the division of the biotypes into sevenmain groups, although the characteristics examined were found to be highly similar and not areadependent.Moreover, all samples were analyzed for their phytochemical content, applying an extractionprotocol to obtain the nonvolatile components and hydrodistillation to collect the essential oils for thevolatile components. The extracts were characterized by LC-UV-DAD/ESI-MS, and the essential oils byGC-FID and GC/MS analyses. In the nonvolatile fractions, 18 components were identified, namely, 13flavones, two organic acids, and three diterpenes. In the volatile fractions, a total of 82 components werefound, with as predominant components a-pinene and camphene among the monoterpene hydrocarbonsand 1,8-cineole, camphor, borneol, and verbenone among the oxygenated monoterpenes. Clusteranalyses were carried out on both phytochemical profiles, allowing the separation of the rosemarysamples into different chemical groups. Finally, the total phenol content and the antioxidant activity ofthe essential oils and extracts were determined with the Folin–Ciocalteu (FC) colorimetric assay, the UVradiation-induced peroxidation in liposomal membranes (UV-IP test), and the scavenging activity of thesuperoxide radical (O2 ). The present study confirmed that the essential oils and organic extracts of theSicilian rosemary samples analyzed showed a considerable antioxidant/free radical-scavenging activity.

UR - http://hdl.handle.net/10447/208383

M3 - Article

VL - 12

SP - 1075

EP - 1093

JO - CHEMISTRY & BIODIVERSITY

JF - CHEMISTRY & BIODIVERSITY

SN - 1612-1872

ER -