Vitamin D3 affects innate immune status of European sea bass (Dicentrarchus labrax L.)

Matteo Cammarata, Mirella Vazzana, Maria Dioguardi, Alberto Cuesta, María Ángeles Esteban, Guardiola

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3 Citazioni (Scopus)

Abstract

The effects of vitamin D3 dietary administration on certain innate immune parameters on the expression of immune-related genes in head-kidney (HK) and gut were investigated in European sea bass Dicentrarchus labrax. Vitamin D3 (vD3) was orally administered to fish in a commercial pellet food supplemented with 0 (control); 3750; 18,750; or 37,500 U kg−1. Furthermore, gut histology was considered. This study showed a modulation in the activities examined in fish fed with the addition of vD3. After just 2 weeks of administration, diet supplementation with the vitamin resulted in increased phagocytic ability, while serum peroxidase content was increased in fish fed with all experimental diets after 4 weeks, no significant differences were observed in protease, anti-protease, natural haemolytic complement activities and total IgM level. At gene level, fbl and rbl transcripts were up-regulated in HK in fish fed with the highest concentration of vD3-supplemented diets after 4 weeks, while in the gut, an up-regulation of hep gene was observed in fish fed with the different doses of vD3. These results suggest that vD3 may be of great interest for immunostimulatory purposes in fish farms.
Lingua originaleEnglish
pagine (da-a)1-14
Numero di pagine14
RivistaFish Physiology and Biochemistry
Stato di pubblicazionePublished - 2017

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Bass
cholecalciferol
Cholecalciferol
Dicentrarchus labrax
vitamin
Fish
Fishes
fish feeds
Nutrition
Head Kidney
fish
digestive system
Genes
Diet
diet
Peptide Hydrolases
proteinases
Complement Hemolytic Activity Assay
gene
kidneys

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Aquatic Science
  • Physiology

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title = "Vitamin D3 affects innate immune status of European sea bass (Dicentrarchus labrax L.)",
abstract = "The effects of vitamin D3 dietary administration on certain innate immune parameters on the expression of immune-related genes in head-kidney (HK) and gut were investigated in European sea bass Dicentrarchus labrax. Vitamin D3 (vD3) was orally administered to fish in a commercial pellet food supplemented with 0 (control); 3750; 18,750; or 37,500 U kg−1. Furthermore, gut histology was considered. This study showed a modulation in the activities examined in fish fed with the addition of vD3. After just 2 weeks of administration, diet supplementation with the vitamin resulted in increased phagocytic ability, while serum peroxidase content was increased in fish fed with all experimental diets after 4 weeks, no significant differences were observed in protease, anti-protease, natural haemolytic complement activities and total IgM level. At gene level, fbl and rbl transcripts were up-regulated in HK in fish fed with the highest concentration of vD3-supplemented diets after 4 weeks, while in the gut, an up-regulation of hep gene was observed in fish fed with the different doses of vD3. These results suggest that vD3 may be of great interest for immunostimulatory purposes in fish farms.",
keywords = "Cholecalciferol (vitamin D3); European sea bass (Dicentrarchus labrax L.); Innate immunity; Teleost; Physiology; Biochemistry; Aquatic Science",
author = "Matteo Cammarata and Mirella Vazzana and Maria Dioguardi and Alberto Cuesta and Esteban, {Mar{\'i}a {\'A}ngeles} and Guardiola",
year = "2017",
language = "English",
pages = "1--14",
journal = "Fish Physiology and Biochemistry",
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TY - JOUR

T1 - Vitamin D3 affects innate immune status of European sea bass (Dicentrarchus labrax L.)

AU - Cammarata, Matteo

AU - Vazzana, Mirella

AU - Dioguardi, Maria

AU - Cuesta, Alberto

AU - Esteban, María Ángeles

AU - Guardiola, null

PY - 2017

Y1 - 2017

N2 - The effects of vitamin D3 dietary administration on certain innate immune parameters on the expression of immune-related genes in head-kidney (HK) and gut were investigated in European sea bass Dicentrarchus labrax. Vitamin D3 (vD3) was orally administered to fish in a commercial pellet food supplemented with 0 (control); 3750; 18,750; or 37,500 U kg−1. Furthermore, gut histology was considered. This study showed a modulation in the activities examined in fish fed with the addition of vD3. After just 2 weeks of administration, diet supplementation with the vitamin resulted in increased phagocytic ability, while serum peroxidase content was increased in fish fed with all experimental diets after 4 weeks, no significant differences were observed in protease, anti-protease, natural haemolytic complement activities and total IgM level. At gene level, fbl and rbl transcripts were up-regulated in HK in fish fed with the highest concentration of vD3-supplemented diets after 4 weeks, while in the gut, an up-regulation of hep gene was observed in fish fed with the different doses of vD3. These results suggest that vD3 may be of great interest for immunostimulatory purposes in fish farms.

AB - The effects of vitamin D3 dietary administration on certain innate immune parameters on the expression of immune-related genes in head-kidney (HK) and gut were investigated in European sea bass Dicentrarchus labrax. Vitamin D3 (vD3) was orally administered to fish in a commercial pellet food supplemented with 0 (control); 3750; 18,750; or 37,500 U kg−1. Furthermore, gut histology was considered. This study showed a modulation in the activities examined in fish fed with the addition of vD3. After just 2 weeks of administration, diet supplementation with the vitamin resulted in increased phagocytic ability, while serum peroxidase content was increased in fish fed with all experimental diets after 4 weeks, no significant differences were observed in protease, anti-protease, natural haemolytic complement activities and total IgM level. At gene level, fbl and rbl transcripts were up-regulated in HK in fish fed with the highest concentration of vD3-supplemented diets after 4 weeks, while in the gut, an up-regulation of hep gene was observed in fish fed with the different doses of vD3. These results suggest that vD3 may be of great interest for immunostimulatory purposes in fish farms.

KW - Cholecalciferol (vitamin D3); European sea bass (Dicentrarchus labrax L.); Innate immunity; Teleost; Physiology; Biochemistry; Aquatic Science

UR - http://hdl.handle.net/10447/229191

UR - http://www.kluweronline.com/issn/0920-1742/

M3 - Article

SP - 1

EP - 14

JO - Fish Physiology and Biochemistry

JF - Fish Physiology and Biochemistry

SN - 0920-1742

ER -