Virologic, hematologic, and immunologic risk factors for classic Kaposi sarcoma.

Francesco Vitale, Carmela Lauria, Christine Gamache, Anthony J. Alberg, Diego Serraino, Denise Whitby, Angelo Messina, Vickie Marshall, Georgina Mbisa, Paola Cordiali-Fei, James J. Goedert, Elizabeth E. Brown

Risultato della ricerca: Article

45 Citazioni (Scopus)

Abstract

BACKGROUND. Classic Kaposi sarcoma (CKS) is an inflammatory-mediated neoplasm that develops in the presence of KS-associated herpesvirus (KSHV) and immune perturbation. In the current study, the authors compared CKS cases with age-matched and sex-matched KSHV-seropositive controls without human immunodeficiency virus-1 infection and markers of viral control, blood counts, CD4-positive and CD8-positive lymphocytes, and serum β-2-microglobulin and neopterin levels. METHODS. Viral loads were detected using real-time amplification of the KSHV-K6 and EBV-pol genes, anti-K8.1 (lytic) titers were detected by enzyme-linked immunoadsorbent assay, and antilatent nuclear antigen (LANA) titers were detected using immunofluorescence. Odds ratios (OR) and 95% confidence intervals (95% CI) were calculated using logistic regression adjusted for sex, age, and study site. RESULTS. Peripheral blood mononuclear cells (PBMC) KSHV DNA detection (P ≤ .0001) and high KSHV lytic (>1:1745; P ≤ .0001) and latent (>1:102,400; P = .03) antibody titers were found to be positively associated with CKS risk. Antibody titers were higher in cases with lesions compared with cases without lesions (P ≤ .05). The detection of Epstein-Barr virus (EBV) DNA in PBMCs was not found to be associated with CKS (P = .95). Independent of PBMC KSHV DNA, CKS risk was found to be positively associated with reduced hematocrit (<37.4%; P = .03), hemoglobin (<12g/dL; P = .04), and lymphocytes (<1000 cells/μL; P = .004), including CD4-positive (+) cells (<457 cells/μL; P = .07) and CD8+ cells (<213cells/μL; P = .04), and with increased monocytes (≥638 cells/μL; P = .009). Nonsignificant elevations of β-2-microglobulin and neopterin were observed among cases regardless of disease burden (P ≥ .08). In a multivariate model, the CKS risk was found to be associated with PBMC KSHV DNA (OR of 2.7; 95% CI, 1.4-5.3), a high KSHV lytic antibody titer (OR of 3.7; 95% CI, 1.9-7.4), and low lymphocytes, particularly among those patients age <70 years (OR of 8.0; 95% CI, 2.7-23.7). CONCLUSIONS. The findings of the current study appear to corroborate the specificity of KSHV and highlight the hematologic and immunologic correlates involved in the pathogenesis of CKS.
Lingua originaleEnglish
pagine (da-a)2282-2290
RivistaCancer
Volume107
Stato di pubblicazionePublished - 2006

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Kaposi's Sarcoma
Herpesviridae
Immunologic Factors
Odds Ratio
Confidence Intervals
Neopterin
beta 2-Microglobulin
Blood Cells
DNA
Human Herpesvirus 4
Antibodies
CD8-Positive T-Lymphocytes
Lymphocytes
pol Genes
Nuclear Antigens
Immunosorbents
Virus Diseases
CD4 Lymphocyte Count
Viral Load
Hematocrit

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Cita questo

Vitale, F., Lauria, C., Gamache, C., Alberg, A. J., Serraino, D., Whitby, D., ... Brown, E. E. (2006). Virologic, hematologic, and immunologic risk factors for classic Kaposi sarcoma. Cancer, 107, 2282-2290.

Virologic, hematologic, and immunologic risk factors for classic Kaposi sarcoma. / Vitale, Francesco; Lauria, Carmela; Gamache, Christine; Alberg, Anthony J.; Serraino, Diego; Whitby, Denise; Messina, Angelo; Marshall, Vickie; Mbisa, Georgina; Cordiali-Fei, Paola; Goedert, James J.; Brown, Elizabeth E.

In: Cancer, Vol. 107, 2006, pag. 2282-2290.

Risultato della ricerca: Article

Vitale, F, Lauria, C, Gamache, C, Alberg, AJ, Serraino, D, Whitby, D, Messina, A, Marshall, V, Mbisa, G, Cordiali-Fei, P, Goedert, JJ & Brown, EE 2006, 'Virologic, hematologic, and immunologic risk factors for classic Kaposi sarcoma.', Cancer, vol. 107, pagg. 2282-2290.
Vitale F, Lauria C, Gamache C, Alberg AJ, Serraino D, Whitby D e altri. Virologic, hematologic, and immunologic risk factors for classic Kaposi sarcoma. Cancer. 2006;107:2282-2290.
Vitale, Francesco ; Lauria, Carmela ; Gamache, Christine ; Alberg, Anthony J. ; Serraino, Diego ; Whitby, Denise ; Messina, Angelo ; Marshall, Vickie ; Mbisa, Georgina ; Cordiali-Fei, Paola ; Goedert, James J. ; Brown, Elizabeth E. / Virologic, hematologic, and immunologic risk factors for classic Kaposi sarcoma. In: Cancer. 2006 ; Vol. 107. pagg. 2282-2290.
@article{514731e7b66e4c718b2a5ceb02a9f585,
title = "Virologic, hematologic, and immunologic risk factors for classic Kaposi sarcoma.",
abstract = "BACKGROUND. Classic Kaposi sarcoma (CKS) is an inflammatory-mediated neoplasm that develops in the presence of KS-associated herpesvirus (KSHV) and immune perturbation. In the current study, the authors compared CKS cases with age-matched and sex-matched KSHV-seropositive controls without human immunodeficiency virus-1 infection and markers of viral control, blood counts, CD4-positive and CD8-positive lymphocytes, and serum β-2-microglobulin and neopterin levels. METHODS. Viral loads were detected using real-time amplification of the KSHV-K6 and EBV-pol genes, anti-K8.1 (lytic) titers were detected by enzyme-linked immunoadsorbent assay, and antilatent nuclear antigen (LANA) titers were detected using immunofluorescence. Odds ratios (OR) and 95{\%} confidence intervals (95{\%} CI) were calculated using logistic regression adjusted for sex, age, and study site. RESULTS. Peripheral blood mononuclear cells (PBMC) KSHV DNA detection (P ≤ .0001) and high KSHV lytic (>1:1745; P ≤ .0001) and latent (>1:102,400; P = .03) antibody titers were found to be positively associated with CKS risk. Antibody titers were higher in cases with lesions compared with cases without lesions (P ≤ .05). The detection of Epstein-Barr virus (EBV) DNA in PBMCs was not found to be associated with CKS (P = .95). Independent of PBMC KSHV DNA, CKS risk was found to be positively associated with reduced hematocrit (<37.4{\%}; P = .03), hemoglobin (<12g/dL; P = .04), and lymphocytes (<1000 cells/μL; P = .004), including CD4-positive (+) cells (<457 cells/μL; P = .07) and CD8+ cells (<213cells/μL; P = .04), and with increased monocytes (≥638 cells/μL; P = .009). Nonsignificant elevations of β-2-microglobulin and neopterin were observed among cases regardless of disease burden (P ≥ .08). In a multivariate model, the CKS risk was found to be associated with PBMC KSHV DNA (OR of 2.7; 95{\%} CI, 1.4-5.3), a high KSHV lytic antibody titer (OR of 3.7; 95{\%} CI, 1.9-7.4), and low lymphocytes, particularly among those patients age <70 years (OR of 8.0; 95{\%} CI, 2.7-23.7). CONCLUSIONS. The findings of the current study appear to corroborate the specificity of KSHV and highlight the hematologic and immunologic correlates involved in the pathogenesis of CKS.",
author = "Francesco Vitale and Carmela Lauria and Christine Gamache and Alberg, {Anthony J.} and Diego Serraino and Denise Whitby and Angelo Messina and Vickie Marshall and Georgina Mbisa and Paola Cordiali-Fei and Goedert, {James J.} and Brown, {Elizabeth E.}",
year = "2006",
language = "English",
volume = "107",
pages = "2282--2290",
journal = "Cancer",
issn = "0008-543X",
publisher = "John Wiley and Sons Inc.",

}

TY - JOUR

T1 - Virologic, hematologic, and immunologic risk factors for classic Kaposi sarcoma.

AU - Vitale, Francesco

AU - Lauria, Carmela

AU - Gamache, Christine

AU - Alberg, Anthony J.

AU - Serraino, Diego

AU - Whitby, Denise

AU - Messina, Angelo

AU - Marshall, Vickie

AU - Mbisa, Georgina

AU - Cordiali-Fei, Paola

AU - Goedert, James J.

AU - Brown, Elizabeth E.

PY - 2006

Y1 - 2006

N2 - BACKGROUND. Classic Kaposi sarcoma (CKS) is an inflammatory-mediated neoplasm that develops in the presence of KS-associated herpesvirus (KSHV) and immune perturbation. In the current study, the authors compared CKS cases with age-matched and sex-matched KSHV-seropositive controls without human immunodeficiency virus-1 infection and markers of viral control, blood counts, CD4-positive and CD8-positive lymphocytes, and serum β-2-microglobulin and neopterin levels. METHODS. Viral loads were detected using real-time amplification of the KSHV-K6 and EBV-pol genes, anti-K8.1 (lytic) titers were detected by enzyme-linked immunoadsorbent assay, and antilatent nuclear antigen (LANA) titers were detected using immunofluorescence. Odds ratios (OR) and 95% confidence intervals (95% CI) were calculated using logistic regression adjusted for sex, age, and study site. RESULTS. Peripheral blood mononuclear cells (PBMC) KSHV DNA detection (P ≤ .0001) and high KSHV lytic (>1:1745; P ≤ .0001) and latent (>1:102,400; P = .03) antibody titers were found to be positively associated with CKS risk. Antibody titers were higher in cases with lesions compared with cases without lesions (P ≤ .05). The detection of Epstein-Barr virus (EBV) DNA in PBMCs was not found to be associated with CKS (P = .95). Independent of PBMC KSHV DNA, CKS risk was found to be positively associated with reduced hematocrit (<37.4%; P = .03), hemoglobin (<12g/dL; P = .04), and lymphocytes (<1000 cells/μL; P = .004), including CD4-positive (+) cells (<457 cells/μL; P = .07) and CD8+ cells (<213cells/μL; P = .04), and with increased monocytes (≥638 cells/μL; P = .009). Nonsignificant elevations of β-2-microglobulin and neopterin were observed among cases regardless of disease burden (P ≥ .08). In a multivariate model, the CKS risk was found to be associated with PBMC KSHV DNA (OR of 2.7; 95% CI, 1.4-5.3), a high KSHV lytic antibody titer (OR of 3.7; 95% CI, 1.9-7.4), and low lymphocytes, particularly among those patients age <70 years (OR of 8.0; 95% CI, 2.7-23.7). CONCLUSIONS. The findings of the current study appear to corroborate the specificity of KSHV and highlight the hematologic and immunologic correlates involved in the pathogenesis of CKS.

AB - BACKGROUND. Classic Kaposi sarcoma (CKS) is an inflammatory-mediated neoplasm that develops in the presence of KS-associated herpesvirus (KSHV) and immune perturbation. In the current study, the authors compared CKS cases with age-matched and sex-matched KSHV-seropositive controls without human immunodeficiency virus-1 infection and markers of viral control, blood counts, CD4-positive and CD8-positive lymphocytes, and serum β-2-microglobulin and neopterin levels. METHODS. Viral loads were detected using real-time amplification of the KSHV-K6 and EBV-pol genes, anti-K8.1 (lytic) titers were detected by enzyme-linked immunoadsorbent assay, and antilatent nuclear antigen (LANA) titers were detected using immunofluorescence. Odds ratios (OR) and 95% confidence intervals (95% CI) were calculated using logistic regression adjusted for sex, age, and study site. RESULTS. Peripheral blood mononuclear cells (PBMC) KSHV DNA detection (P ≤ .0001) and high KSHV lytic (>1:1745; P ≤ .0001) and latent (>1:102,400; P = .03) antibody titers were found to be positively associated with CKS risk. Antibody titers were higher in cases with lesions compared with cases without lesions (P ≤ .05). The detection of Epstein-Barr virus (EBV) DNA in PBMCs was not found to be associated with CKS (P = .95). Independent of PBMC KSHV DNA, CKS risk was found to be positively associated with reduced hematocrit (<37.4%; P = .03), hemoglobin (<12g/dL; P = .04), and lymphocytes (<1000 cells/μL; P = .004), including CD4-positive (+) cells (<457 cells/μL; P = .07) and CD8+ cells (<213cells/μL; P = .04), and with increased monocytes (≥638 cells/μL; P = .009). Nonsignificant elevations of β-2-microglobulin and neopterin were observed among cases regardless of disease burden (P ≥ .08). In a multivariate model, the CKS risk was found to be associated with PBMC KSHV DNA (OR of 2.7; 95% CI, 1.4-5.3), a high KSHV lytic antibody titer (OR of 3.7; 95% CI, 1.9-7.4), and low lymphocytes, particularly among those patients age <70 years (OR of 8.0; 95% CI, 2.7-23.7). CONCLUSIONS. The findings of the current study appear to corroborate the specificity of KSHV and highlight the hematologic and immunologic correlates involved in the pathogenesis of CKS.

UR - http://hdl.handle.net/10447/18834

M3 - Article

VL - 107

SP - 2282

EP - 2290

JO - Cancer

JF - Cancer

SN - 0008-543X

ER -