TY - JOUR
T1 - Vascular endothelial growth factor induces brain erythropoietin expression?
AU - Grasso, Giovanni
AU - Bellomo, Maria
AU - Grasso, Giovanni
AU - Marini, Rolando
AU - Adamo, Elena B.
AU - Mannucci, Carmen
AU - Marini, Herbert
AU - Giuffrida, Rosario
AU - Catania, Maria A.
AU - Calapai, Gioacchino
AU - Squadrito, Francesco
AU - Giacca, Mauro
AU - Buemi, Michele
AU - Marini, Rolando
AU - Caputi, Achille P.
AU - Bellomo, Marcello
AU - Catania, Mariavalentina
PY - 2006
Y1 - 2006
N2 - To investigate whether the formation of vascular endothelial growth factor (VEGF) influences erythropoietin (EPO) expression in physiological conditions, we injected into the left lateral cerebral ventricle of the Mongolian gerbil an adeno-associated virus (AAV) vector capable of expressing the 165-amino-acid isoform of VEGF (VEGF165). Twelve and 18 days after AAV vector injection, the experimental animals were sacrificed and expression of EPO was evaluated through immunohistochemical analysis of both the hippocampus and the frontal cortex. We observed that VEGF165 induces EPO expression in the hippocampal pyramidal layers and in the frontal cortex of the gerbil, particularly after the 18th day following treatment with the vector, which suggests that VEGF165 could act as a hypoxic-like signal for EPO production. This finding could help to clarify the functional role of EPO and the molecular mechanisms by which VEGF might mediate its effects in the brain.
AB - To investigate whether the formation of vascular endothelial growth factor (VEGF) influences erythropoietin (EPO) expression in physiological conditions, we injected into the left lateral cerebral ventricle of the Mongolian gerbil an adeno-associated virus (AAV) vector capable of expressing the 165-amino-acid isoform of VEGF (VEGF165). Twelve and 18 days after AAV vector injection, the experimental animals were sacrificed and expression of EPO was evaluated through immunohistochemical analysis of both the hippocampus and the frontal cortex. We observed that VEGF165 induces EPO expression in the hippocampal pyramidal layers and in the frontal cortex of the gerbil, particularly after the 18th day following treatment with the vector, which suggests that VEGF165 could act as a hypoxic-like signal for EPO production. This finding could help to clarify the functional role of EPO and the molecular mechanisms by which VEGF might mediate its effects in the brain.
UR - http://hdl.handle.net/10447/26741
M3 - Article
VL - 21
SP - 87
EP - 91
JO - Functional Neurology
JF - Functional Neurology
SN - 0393-5264
ER -