Utility of laser microdissection and pressure catapulting in the diagnosis of non small cell lung cancer: preliminary data.

Massimo Cajozzo, Viviana Bazan, Antonio Russo, Damiano Calvo, Mario Barone, Marcello Migliore

Risultato della ricerca: Article

Abstract

Background: There are controversies about the adequacy of tumor tissue sample on which the sequencing of molecular diagnosiscould be performed to achieve the targeted-therapy on lung cancer. The aim of this study is to demonstrate the role of the LaserMicrodissection Pressure Catapulting (LMPC) technique to obtain adequate tumor tissue sample for the molecular analysis of genemutations in the target therapy of lung cancer.Findings: From a consecutive series of 24 patients with a diagnosis of locally-advanced or metastatic Non Small Cell LungCancer (NSCLC), we performed 29 diagnostic procedures using the system of LMPC, to obtain an homogeneous samples where itwas possible to run the sequencing of the 4 most frequently mutated exons of Epidermal Growth Factor Receptor (EGFR) (exon 18,19, 20, 21).Results: There were 14 males (58.3%) and 10 females (41.7%), with a mean age of 61 years old. Twenty one patients wereaffected by adenocarcinomas, 2 by squamous cell carcinomas and 1 by large cell carcinoma. We were able to obtain the sequencingon 26 out 29 samples (89,6%) for EGFR mutation. EGFR mutation rate in our population was 7,7%. In 5 samples, we found a polymorphismin exon 20 and one of them carried a mutation on exon 18 as well. In another sample we found the deletion of exon 19. Onthe other 20 samples we did not find any mutation.Conclusions: Our preliminary data suggest that the LMPC technique permits to obtain the tumor cells sample more homogeneousfacilitating the application of biological molecular analysis for EGFR-gene mutation in a larger number of patients withNSCLC.
Lingua originaleEnglish
pagine (da-a)237-240
Numero di pagine4
RivistaActa Medica Mediterranea
Volume30
Stato di pubblicazionePublished - 2014

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Microdissection
Non-Small Cell Lung Carcinoma
Exons
Lasers
Pressure
Epidermal Growth Factor Receptor
Mutation
Lung Neoplasms
erbB-1 Genes
Large Cell Carcinoma
Neoplasms
Mutation Rate
Squamous Cell Carcinoma
Adenocarcinoma
Therapeutics
Population

All Science Journal Classification (ASJC) codes

  • Medicine(all)

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@article{e1d628c90d9e4cee88ea96f1bc828e56,
title = "Utility of laser microdissection and pressure catapulting in the diagnosis of non small cell lung cancer: preliminary data.",
abstract = "Background: There are controversies about the adequacy of tumor tissue sample on which the sequencing of molecular diagnosiscould be performed to achieve the targeted-therapy on lung cancer. The aim of this study is to demonstrate the role of the LaserMicrodissection Pressure Catapulting (LMPC) technique to obtain adequate tumor tissue sample for the molecular analysis of genemutations in the target therapy of lung cancer.Findings: From a consecutive series of 24 patients with a diagnosis of locally-advanced or metastatic Non Small Cell LungCancer (NSCLC), we performed 29 diagnostic procedures using the system of LMPC, to obtain an homogeneous samples where itwas possible to run the sequencing of the 4 most frequently mutated exons of Epidermal Growth Factor Receptor (EGFR) (exon 18,19, 20, 21).Results: There were 14 males (58.3{\%}) and 10 females (41.7{\%}), with a mean age of 61 years old. Twenty one patients wereaffected by adenocarcinomas, 2 by squamous cell carcinomas and 1 by large cell carcinoma. We were able to obtain the sequencingon 26 out 29 samples (89,6{\%}) for EGFR mutation. EGFR mutation rate in our population was 7,7{\%}. In 5 samples, we found a polymorphismin exon 20 and one of them carried a mutation on exon 18 as well. In another sample we found the deletion of exon 19. Onthe other 20 samples we did not find any mutation.Conclusions: Our preliminary data suggest that the LMPC technique permits to obtain the tumor cells sample more homogeneousfacilitating the application of biological molecular analysis for EGFR-gene mutation in a larger number of patients withNSCLC.",
author = "Massimo Cajozzo and Viviana Bazan and Antonio Russo and Damiano Calvo and Mario Barone and Marcello Migliore",
year = "2014",
language = "English",
volume = "30",
pages = "237--240",
journal = "Acta Medica Mediterranea",
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publisher = "A. CARBONE Editore",

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TY - JOUR

T1 - Utility of laser microdissection and pressure catapulting in the diagnosis of non small cell lung cancer: preliminary data.

AU - Cajozzo, Massimo

AU - Bazan, Viviana

AU - Russo, Antonio

AU - Calvo, Damiano

AU - Barone, Mario

AU - Migliore, Marcello

PY - 2014

Y1 - 2014

N2 - Background: There are controversies about the adequacy of tumor tissue sample on which the sequencing of molecular diagnosiscould be performed to achieve the targeted-therapy on lung cancer. The aim of this study is to demonstrate the role of the LaserMicrodissection Pressure Catapulting (LMPC) technique to obtain adequate tumor tissue sample for the molecular analysis of genemutations in the target therapy of lung cancer.Findings: From a consecutive series of 24 patients with a diagnosis of locally-advanced or metastatic Non Small Cell LungCancer (NSCLC), we performed 29 diagnostic procedures using the system of LMPC, to obtain an homogeneous samples where itwas possible to run the sequencing of the 4 most frequently mutated exons of Epidermal Growth Factor Receptor (EGFR) (exon 18,19, 20, 21).Results: There were 14 males (58.3%) and 10 females (41.7%), with a mean age of 61 years old. Twenty one patients wereaffected by adenocarcinomas, 2 by squamous cell carcinomas and 1 by large cell carcinoma. We were able to obtain the sequencingon 26 out 29 samples (89,6%) for EGFR mutation. EGFR mutation rate in our population was 7,7%. In 5 samples, we found a polymorphismin exon 20 and one of them carried a mutation on exon 18 as well. In another sample we found the deletion of exon 19. Onthe other 20 samples we did not find any mutation.Conclusions: Our preliminary data suggest that the LMPC technique permits to obtain the tumor cells sample more homogeneousfacilitating the application of biological molecular analysis for EGFR-gene mutation in a larger number of patients withNSCLC.

AB - Background: There are controversies about the adequacy of tumor tissue sample on which the sequencing of molecular diagnosiscould be performed to achieve the targeted-therapy on lung cancer. The aim of this study is to demonstrate the role of the LaserMicrodissection Pressure Catapulting (LMPC) technique to obtain adequate tumor tissue sample for the molecular analysis of genemutations in the target therapy of lung cancer.Findings: From a consecutive series of 24 patients with a diagnosis of locally-advanced or metastatic Non Small Cell LungCancer (NSCLC), we performed 29 diagnostic procedures using the system of LMPC, to obtain an homogeneous samples where itwas possible to run the sequencing of the 4 most frequently mutated exons of Epidermal Growth Factor Receptor (EGFR) (exon 18,19, 20, 21).Results: There were 14 males (58.3%) and 10 females (41.7%), with a mean age of 61 years old. Twenty one patients wereaffected by adenocarcinomas, 2 by squamous cell carcinomas and 1 by large cell carcinoma. We were able to obtain the sequencingon 26 out 29 samples (89,6%) for EGFR mutation. EGFR mutation rate in our population was 7,7%. In 5 samples, we found a polymorphismin exon 20 and one of them carried a mutation on exon 18 as well. In another sample we found the deletion of exon 19. Onthe other 20 samples we did not find any mutation.Conclusions: Our preliminary data suggest that the LMPC technique permits to obtain the tumor cells sample more homogeneousfacilitating the application of biological molecular analysis for EGFR-gene mutation in a larger number of patients withNSCLC.

UR - http://hdl.handle.net/10447/98482

M3 - Article

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EP - 240

JO - Acta Medica Mediterranea

JF - Acta Medica Mediterranea

SN - 0393-6384

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