Unjamming overcomes kinetic and proliferation arrest in terminally differentiated cells and promotes collective motility of carcinoma

Claudio Tripodo, Sara Sigismund, Fabio Giavazzi, Massimiliano Garre, Salvatore Corallino, Qingsen Li, Elisabetta A. Cavalcanti-Adam, Flora Ascione, Emanuela Frittoli, Elisa Barbieri, Emanuele Martini, Chiara Malinverno, Claudio Tripodo, Galina V. Beznoussenko, Andrea Palamidessi, Roberto Cerbino, Giorgio Scita, Pier Paolo Di Fiore, Dario Parazzoli

Risultato della ricerca: Articlepeer review

35 Citazioni (Scopus)

Abstract

During wound repair, branching morphogenesis and carcinoma dissemination, cellular rearrangements are fostered by a solid-to-liquid transition, known as unjamming. The biomolecular machinery behind unjamming and its pathophysiological relevance remain, however, unclear. Here, we study unjamming in a variety of normal and tumorigenic epithelial two-dimensional (2D) and 3D collectives. Biologically, the increased level of the small GTPase RAB5A sparks unjamming by promoting non-clathrin-dependent internalization of epidermal growth factor receptor that leads to hyperactivation of the kinase ERK1/2 and phosphorylation of the actin nucleator WAVE2. This cascade triggers collective motility effects with striking biophysical consequences. Specifically, unjamming in tumour spheroids is accompanied by persistent and coordinated rotations that progressively remodel the extracellular matrix, while simultaneously fluidizing cells at the periphery. This concurrent action results in collective invasion, supporting the concept that the endo-ERK1/2 pathway is a physicochemical switch to initiate collective invasion and dissemination of otherwise jammed carcinoma.
Lingua originaleEnglish
pagine (da-a)1252-1263
Numero di pagine12
RivistaNature Materials
Volume18
Stato di pubblicazionePublished - 2019

All Science Journal Classification (ASJC) codes

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  • ???subjectarea.asjc.2500.2500???
  • ???subjectarea.asjc.3100.3104???
  • ???subjectarea.asjc.2200.2211???
  • ???subjectarea.asjc.2200.2210???

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