Abstract
The fluorescence-based sensing capability of ultrathin ZnO-SiO(2) nanoplatforms, deposited by an integrated approach of colloidal lithography and metal organic chemical vapor deposition, has been investigated upon adsorption of fluorescein-labeled albumin, used as model analyte biomolecule. The protein immobilization process after spontaneous adsorption/desorption significantly enhances the green emission of the different ZnO-based films, as evidenced by scanning confocal microscopy, corresponding to a comparable protein coverage detected by X-ray photoelectron spectroscopy. Moreover, experiments of fluorescence recovery after photobleaching evidence that the protein lateral diffusion at the biointerface is affected by the chemical and/or topographical patterning of hybrid ZnO-SiO(2) surfaces. The used approach is very promising for biomolecular detection applications of these ZnO-SiO(2) nanoplatforms, by simple sizing of the 2D vs. 3D patterning design, which in turn is accomplished by the fine tuning of the integrated colloidal lithography-chemical vapor deposition processes. (C) 2011 Elsevier Inc. All rights reserved.
Lingua originale | English |
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pagine (da-a) | 90-96 |
Numero di pagine | 7 |
Rivista | Journal of Colloid and Interface Science |
Volume | 365 |
Stato di pubblicazione | Published - 2012 |
All Science Journal Classification (ASJC) codes
- Electronic, Optical and Magnetic Materials
- Biomaterials
- Surfaces, Coatings and Films
- Colloid and Surface Chemistry