Ultrastructural changes in the Interstitials Cells of Cajal and gastric dysrhythmias in mice lacking full-length dystrophin (mdx mice)

Maria Grazia Zizzo, Flavia Mule', Rosa Maria Serio, Laura Pieri, Claudio Zardo, Maria-Giuliana Vannucchi, Maria-Simonetta Faussone-Pellegrini

Risultato della ricerca: Article

20 Citazioni (Scopus)

Abstract

At least two populations of c-kit positive interstitial cells of Cajal (ICC) lie in thegastric wall, one located at the myenteric plexus level has a pace-making functionand the other located intramuscularly is intermediary in the neurotransmission andregenerates the slow waves. Both of these ICC sub-types express full-lengthdystrophin. Mdx mice, an animal model lacking in full-length dystrophin and usedto study Duchenne muscular dystrophy (DMD), show gastric dismotilities. The aimof the present study was to verify in mdx mice whether: (i) gastric ICC undergomorphological changes, through immunohistochemical and ultrastructural analyses;and (ii) there are alterations in the electrical activity, using intracellularrecording technique. In control mice, ICC sub-types showed heterogeneousultrastructural features, either intramuscularly or at the myenteric plexus level. Inmdx mice, all of the ICC sub-types underwent important changes: coated vesicleswere significantly more numerous and caveolae significantly fewer than in control;moreover, cytoskeleton and smooth endoplasmic reticulum were reduced andmitochondria enlarged. c-Kit-positivity and integrity of the ICC networks weremaintained. In the circular muscle of normal mice slow waves, which consisted ofinitial and secondary components, occurred with a regular frequency. In mdx mice,slow waves occurred in a highly dysrhythmic fashion and they lacked a secondarycomponent. We conclude that the lack of the full-length dystrophin is associatedwith ultrastructural modifications of gastric ICC, most of which can be interpretedas signs of new membrane formation and altered Ca2þ handling, and with defectivegeneration and regeneration of slow wave activity.
Lingua originaleEnglish
pagine (da-a)293-309
Numero di pagine17
RivistaJournal of Cellular Physiology
Volume199
Stato di pubblicazionePublished - 2004

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Interstitial Cells of Cajal
Inbred mdx Mouse
Dystrophin
Stomach
Cells
Myenteric Plexus
Muscle
Animals
Smooth Endoplasmic Reticulum
Caveolae
Membranes
Duchenne Muscular Dystrophy
Cytoskeleton
Synaptic Transmission
Regeneration
Animal Models
Muscles

All Science Journal Classification (ASJC) codes

  • Physiology
  • Clinical Biochemistry
  • Cell Biology

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Ultrastructural changes in the Interstitials Cells of Cajal and gastric dysrhythmias in mice lacking full-length dystrophin (mdx mice). / Zizzo, Maria Grazia; Mule', Flavia; Serio, Rosa Maria; Pieri, Laura; Zardo, Claudio; Vannucchi, Maria-Giuliana; Faussone-Pellegrini, Maria-Simonetta.

In: Journal of Cellular Physiology, Vol. 199, 2004, pag. 293-309.

Risultato della ricerca: Article

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title = "Ultrastructural changes in the Interstitials Cells of Cajal and gastric dysrhythmias in mice lacking full-length dystrophin (mdx mice)",
abstract = "At least two populations of c-kit positive interstitial cells of Cajal (ICC) lie in thegastric wall, one located at the myenteric plexus level has a pace-making functionand the other located intramuscularly is intermediary in the neurotransmission andregenerates the slow waves. Both of these ICC sub-types express full-lengthdystrophin. Mdx mice, an animal model lacking in full-length dystrophin and usedto study Duchenne muscular dystrophy (DMD), show gastric dismotilities. The aimof the present study was to verify in mdx mice whether: (i) gastric ICC undergomorphological changes, through immunohistochemical and ultrastructural analyses;and (ii) there are alterations in the electrical activity, using intracellularrecording technique. In control mice, ICC sub-types showed heterogeneousultrastructural features, either intramuscularly or at the myenteric plexus level. Inmdx mice, all of the ICC sub-types underwent important changes: coated vesicleswere significantly more numerous and caveolae significantly fewer than in control;moreover, cytoskeleton and smooth endoplasmic reticulum were reduced andmitochondria enlarged. c-Kit-positivity and integrity of the ICC networks weremaintained. In the circular muscle of normal mice slow waves, which consisted ofinitial and secondary components, occurred with a regular frequency. In mdx mice,slow waves occurred in a highly dysrhythmic fashion and they lacked a secondarycomponent. We conclude that the lack of the full-length dystrophin is associatedwith ultrastructural modifications of gastric ICC, most of which can be interpretedas signs of new membrane formation and altered Ca2{\th} handling, and with defectivegeneration and regeneration of slow wave activity.",
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T1 - Ultrastructural changes in the Interstitials Cells of Cajal and gastric dysrhythmias in mice lacking full-length dystrophin (mdx mice)

AU - Zizzo, Maria Grazia

AU - Mule', Flavia

AU - Serio, Rosa Maria

AU - Pieri, Laura

AU - Zardo, Claudio

AU - Vannucchi, Maria-Giuliana

AU - Faussone-Pellegrini, Maria-Simonetta

PY - 2004

Y1 - 2004

N2 - At least two populations of c-kit positive interstitial cells of Cajal (ICC) lie in thegastric wall, one located at the myenteric plexus level has a pace-making functionand the other located intramuscularly is intermediary in the neurotransmission andregenerates the slow waves. Both of these ICC sub-types express full-lengthdystrophin. Mdx mice, an animal model lacking in full-length dystrophin and usedto study Duchenne muscular dystrophy (DMD), show gastric dismotilities. The aimof the present study was to verify in mdx mice whether: (i) gastric ICC undergomorphological changes, through immunohistochemical and ultrastructural analyses;and (ii) there are alterations in the electrical activity, using intracellularrecording technique. In control mice, ICC sub-types showed heterogeneousultrastructural features, either intramuscularly or at the myenteric plexus level. Inmdx mice, all of the ICC sub-types underwent important changes: coated vesicleswere significantly more numerous and caveolae significantly fewer than in control;moreover, cytoskeleton and smooth endoplasmic reticulum were reduced andmitochondria enlarged. c-Kit-positivity and integrity of the ICC networks weremaintained. In the circular muscle of normal mice slow waves, which consisted ofinitial and secondary components, occurred with a regular frequency. In mdx mice,slow waves occurred in a highly dysrhythmic fashion and they lacked a secondarycomponent. We conclude that the lack of the full-length dystrophin is associatedwith ultrastructural modifications of gastric ICC, most of which can be interpretedas signs of new membrane formation and altered Ca2þ handling, and with defectivegeneration and regeneration of slow wave activity.

AB - At least two populations of c-kit positive interstitial cells of Cajal (ICC) lie in thegastric wall, one located at the myenteric plexus level has a pace-making functionand the other located intramuscularly is intermediary in the neurotransmission andregenerates the slow waves. Both of these ICC sub-types express full-lengthdystrophin. Mdx mice, an animal model lacking in full-length dystrophin and usedto study Duchenne muscular dystrophy (DMD), show gastric dismotilities. The aimof the present study was to verify in mdx mice whether: (i) gastric ICC undergomorphological changes, through immunohistochemical and ultrastructural analyses;and (ii) there are alterations in the electrical activity, using intracellularrecording technique. In control mice, ICC sub-types showed heterogeneousultrastructural features, either intramuscularly or at the myenteric plexus level. Inmdx mice, all of the ICC sub-types underwent important changes: coated vesicleswere significantly more numerous and caveolae significantly fewer than in control;moreover, cytoskeleton and smooth endoplasmic reticulum were reduced andmitochondria enlarged. c-Kit-positivity and integrity of the ICC networks weremaintained. In the circular muscle of normal mice slow waves, which consisted ofinitial and secondary components, occurred with a regular frequency. In mdx mice,slow waves occurred in a highly dysrhythmic fashion and they lacked a secondarycomponent. We conclude that the lack of the full-length dystrophin is associatedwith ultrastructural modifications of gastric ICC, most of which can be interpretedas signs of new membrane formation and altered Ca2þ handling, and with defectivegeneration and regeneration of slow wave activity.

UR - http://hdl.handle.net/10447/14296

M3 - Article

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EP - 309

JO - Journal of Cellular Physiology

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SN - 0021-9541

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