Ultrasound-guided intra-tumor injection of combined immunotherapy cures mice from orthotopic prostate cancer.

Claudio Tripodo, Giorgio Mauri, Ivano Arioli, Claudia Chiodoni, Mariella Parenza, Mario Paolo Colombo

Risultato della ricerca: Article

1 Citazione (Scopus)

Abstract

Intra-tumor injection of immunotherapeutic agents is often the most effective, likely because of concomitant modification of tumor microenvironment. We tested an immunotherapeutic regimen consisting of CpG oligonucleotides and of adenovirus-mediated gene delivery of CCL16 chemokine directly into orthotopically implanted prostate tumors by ultrasound-guided injection, followed by systemic administration of an anti-IL-10R antibody. This combination treatment induced rapid stromal rearrangement, characterized by massive leukocyte infiltration and large areas of necrosis, a scenario that eventually led to complete tumor rejection and systemic immunity in 75 % of the treated mice. In vivo T lymphocyte depletion experiments demonstrated that the efficacy of CCL16/CpG/anti-IL-10R combination treatment relies upon CD8 T lymphocytes whereas CD4 T cells are dispensable. The results underlie the feasibility of echo-guided local immunotherapy of tumors located in visceral organs that are not easily accessible
Lingua originaleEnglish
pagine (da-a)1811-1819
Numero di pagine9
RivistaCANCER IMMUNOLOGY, IMMUNOTHERAPY
Volume12
Stato di pubblicazionePublished - 2013

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Immunotherapy
Prostatic Neoplasms
Injections
T-Lymphocytes
Neoplasms
Lymphocyte Depletion
Tumor Microenvironment
Chemokines
Adenoviridae
Oligonucleotides
Prostate
Immunity
Leukocytes
Necrosis
Antibodies
Therapeutics
Genes

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology
  • Oncology
  • Cancer Research

Cita questo

Ultrasound-guided intra-tumor injection of combined immunotherapy cures mice from orthotopic prostate cancer. / Tripodo, Claudio; Mauri, Giorgio; Arioli, Ivano; Chiodoni, Claudia; Parenza, Mariella; Colombo, Mario Paolo.

In: CANCER IMMUNOLOGY, IMMUNOTHERAPY, Vol. 12, 2013, pag. 1811-1819.

Risultato della ricerca: Article

Tripodo, Claudio ; Mauri, Giorgio ; Arioli, Ivano ; Chiodoni, Claudia ; Parenza, Mariella ; Colombo, Mario Paolo. / Ultrasound-guided intra-tumor injection of combined immunotherapy cures mice from orthotopic prostate cancer. In: CANCER IMMUNOLOGY, IMMUNOTHERAPY. 2013 ; Vol. 12. pagg. 1811-1819.
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AU - Tripodo, Claudio

AU - Mauri, Giorgio

AU - Arioli, Ivano

AU - Chiodoni, Claudia

AU - Parenza, Mariella

AU - Colombo, Mario Paolo

PY - 2013

Y1 - 2013

N2 - Intra-tumor injection of immunotherapeutic agents is often the most effective, likely because of concomitant modification of tumor microenvironment. We tested an immunotherapeutic regimen consisting of CpG oligonucleotides and of adenovirus-mediated gene delivery of CCL16 chemokine directly into orthotopically implanted prostate tumors by ultrasound-guided injection, followed by systemic administration of an anti-IL-10R antibody. This combination treatment induced rapid stromal rearrangement, characterized by massive leukocyte infiltration and large areas of necrosis, a scenario that eventually led to complete tumor rejection and systemic immunity in 75 % of the treated mice. In vivo T lymphocyte depletion experiments demonstrated that the efficacy of CCL16/CpG/anti-IL-10R combination treatment relies upon CD8 T lymphocytes whereas CD4 T cells are dispensable. The results underlie the feasibility of echo-guided local immunotherapy of tumors located in visceral organs that are not easily accessible

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