We previously reported that ductal infiltrating carcinomas (d.i.c.) of the human breast display profound modificationsof the stromal architecture, associated with anomalous collagen composition. Among the major alterationsobserved in the interstitial collagen, the relative increase of type V collagen content was detected. When type Vcollagen was used as an ‘‘in vitro’’ substrate for 8701-BC d.i.c. cells, it appeared able to restrain cell growth, inhibitcell motility and invasion ‘‘in vitro’’, and modify the expression levels of genes coding for apoptosis factors, caspasesand stress response proteins. In the present paper we demonstrate that type V collagen induces the down-regulationof protein kinase C eta, an event that may be, at least in part, responsible of the previously-reported modifications ofcell morphology and growth rate, and that appears to be involved in the already-observed changes of expression levelsof genes encoding for anti- (Bcl-2) and pro-apoptotic factors (Bad, Dapk, Bcl-Xs) and enzymes (caspase 5 and 8).
|Numero di pagine||11|
|Stato di pubblicazione||Published - 2013|
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