Two distinct extracellular RNA signatures released by a single cell type identified by microarray and next-generation sequencing

Stefania Raimondo, Kendall Van Keuren Jensen, Dae-Kyum Kim, Rossella Crescitelli, Cecilia Lässer, Yong Song Gho, Stefania Raimondo, Ashish Yeri, Ganesh Vilas Shelke, Jan Lötvall, Margareta Sjöstrand

Risultato della ricerca: Article

52 Citazioni (Scopus)

Abstract

Cells secrete extracellular RNA (exRNA) to their surrounding environment and exRNA has been found in many body fluids such as blood, breast milk and cerebrospinal fluid. However, there are conflicting results regarding the nature of exRNA. Here, we have separated two distinct exRNA profiles released by mast cells, here termed high-density (HD) and low-density (LD) exRNA. The exRNA in both fractions was characterized by microarray and next-generation sequencing. Both exRNA fractions contained mRNA and miRNA, and the mRNAs in the LD exRNA correlated closely with the cellular mRNA, whereas the HD mRNA did not. Furthermore, the HD exRNA was enriched in lincRNA, antisense RNA, vault RNA, snoRNA, and snRNA with little or no evidence of full-length 18S and 28S rRNA. The LD exRNA was enriched in mitochondrial rRNA, mitochondrial tRNA, tRNA, piRNA, Y RNA, and full-length 18S and 28S rRNA. The proteomes of the HD and LD exRNA-containing fractions were determined with LC-MS/MS and analysed with Gene Ontology term finder, which showed that both proteomes were associated with the term extracellular vesicles and electron microscopy suggests that at least a part of the exRNA is associated with exosome-like extracellular vesicles. Additionally, the proteins in the HD fractions tended to be associated with the nucleus and ribosomes, whereas the LD fraction proteome tended to be associated with the mitochondrion. We show that the two exRNA signatures released by a single cell type can be separated by floatation on a density gradient. These results show that cells can release multiple types of exRNA with substantial differences in RNA species content. This is important for any future studies determining the nature and function of exRNA released from different cells under different conditions.
Lingua originaleEnglish
pagine (da-a)58-72
Numero di pagine15
RivistaRNA Biology
Volume14
Stato di pubblicazionePublished - 2016

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

Fingerprint Entra nei temi di ricerca di 'Two distinct extracellular RNA signatures released by a single cell type identified by microarray and next-generation sequencing'. Insieme formano una fingerprint unica.

  • Cita questo

    Raimondo, S., Van Keuren Jensen, K., Kim, D-K., Crescitelli, R., Lässer, C., Gho, Y. S., Raimondo, S., Yeri, A., Shelke, G. V., Lötvall, J., & Sjöstrand, M. (2016). Two distinct extracellular RNA signatures released by a single cell type identified by microarray and next-generation sequencing. RNA Biology, 14, 58-72.