Transcriptome profiling of citrus fruit response to Huanglongbing disease.

Federico Martinelli, Elizabeth Leicht, Raissa D'Souza, Russell L. Reagan, Abhaya M. Dandekar, My L. Phu, Monica Britton, Vincent Buffalo, Federico Martinelli, Ute Albrecht, Sandra L. Uratsu, Joseph Fass, Kim D. Bowman, Weixiang Zhao, Cristina E. Davis, Dawei Lin

Risultato della ricerca: Article

94 Citazioni (Scopus)

Abstract

Huanglongbing (HLB) or "citrus greening" is the most destructive citrus disease worldwide. In this work, we studied host responses of citrus to infection with Candidatus Liberibacter asiaticus (CaLas) using next-generation sequencing technologies. A deep mRNA profile was obtained from peel of healthy and HLB- affected fruit. It was followed by pathway and protein-protein network analysis and quantitative real time PCR analysis of highly regulated genes. We identified differentially regulated pathways and constructed networks that provide a deep insight into the metabolism of affected fruit. Data mining revealed that HLB enhanced transcription of genes involved in the light reactions of photosynthesis and in ATP synthesis. Activation of protein degradation and misfolding processes were observed at the transcriptomic level. Transcripts for heat shock proteins were down-regulated at all disease stages, resulting in further protein misfolding. HLB strongly affected pathways involved in source-sink communication, including sucrose and starch metabolism and hormone synthesis and signaling. Transcription of several genes involved in the synthesis and signal transduction of cytokinins and gibberellins was repressed while that of genes involved in ethylene pathways was induced. CaLas infection triggered a response via both the salicylic acid and jasmonic acid pathways and increased the transcript abundance of several members of the WRKY family of transcription factors. Findings focused on the fruit provide valuable insight to understanding the mechanisms of the HLB-induced fruit disorder and eventually developing methods based on small molecule applications to mitigate its devastating effects on fruit production.
Lingua originaleEnglish
Numero di pagine12
RivistaPLoS One
Volume7
Stato di pubblicazionePublished - 2012

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Citrus fruits
greening disease
Citrus
citrus fruits
Gene Expression Profiling
Fruits
transcriptomics
Fruit
Genes
Candidatus Liberibacter asiaticus
fruits
Transcription
Metabolism
synthesis
Proteins
genes
transcription (genetics)
Gibberellins
Cytokinins
Signal transduction

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

Cita questo

Martinelli, F., Leicht, E., D'Souza, R., Reagan, R. L., Dandekar, A. M., Phu, M. L., ... Lin, D. (2012). Transcriptome profiling of citrus fruit response to Huanglongbing disease. PLoS One, 7.

Transcriptome profiling of citrus fruit response to Huanglongbing disease. / Martinelli, Federico; Leicht, Elizabeth; D'Souza, Raissa; Reagan, Russell L.; Dandekar, Abhaya M.; Phu, My L.; Britton, Monica; Buffalo, Vincent; Martinelli, Federico; Albrecht, Ute; Uratsu, Sandra L.; Fass, Joseph; Bowman, Kim D.; Zhao, Weixiang; Davis, Cristina E.; Lin, Dawei.

In: PLoS One, Vol. 7, 2012.

Risultato della ricerca: Article

Martinelli, F, Leicht, E, D'Souza, R, Reagan, RL, Dandekar, AM, Phu, ML, Britton, M, Buffalo, V, Martinelli, F, Albrecht, U, Uratsu, SL, Fass, J, Bowman, KD, Zhao, W, Davis, CE & Lin, D 2012, 'Transcriptome profiling of citrus fruit response to Huanglongbing disease.', PLoS One, vol. 7.
Martinelli F, Leicht E, D'Souza R, Reagan RL, Dandekar AM, Phu ML e altri. Transcriptome profiling of citrus fruit response to Huanglongbing disease. PLoS One. 2012;7.
Martinelli, Federico ; Leicht, Elizabeth ; D'Souza, Raissa ; Reagan, Russell L. ; Dandekar, Abhaya M. ; Phu, My L. ; Britton, Monica ; Buffalo, Vincent ; Martinelli, Federico ; Albrecht, Ute ; Uratsu, Sandra L. ; Fass, Joseph ; Bowman, Kim D. ; Zhao, Weixiang ; Davis, Cristina E. ; Lin, Dawei. / Transcriptome profiling of citrus fruit response to Huanglongbing disease. In: PLoS One. 2012 ; Vol. 7.
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AU - Martinelli, Federico

AU - Leicht, Elizabeth

AU - D'Souza, Raissa

AU - Reagan, Russell L.

AU - Dandekar, Abhaya M.

AU - Phu, My L.

AU - Britton, Monica

AU - Buffalo, Vincent

AU - Martinelli, Federico

AU - Albrecht, Ute

AU - Uratsu, Sandra L.

AU - Fass, Joseph

AU - Bowman, Kim D.

AU - Zhao, Weixiang

AU - Davis, Cristina E.

AU - Lin, Dawei

PY - 2012

Y1 - 2012

N2 - Huanglongbing (HLB) or "citrus greening" is the most destructive citrus disease worldwide. In this work, we studied host responses of citrus to infection with Candidatus Liberibacter asiaticus (CaLas) using next-generation sequencing technologies. A deep mRNA profile was obtained from peel of healthy and HLB- affected fruit. It was followed by pathway and protein-protein network analysis and quantitative real time PCR analysis of highly regulated genes. We identified differentially regulated pathways and constructed networks that provide a deep insight into the metabolism of affected fruit. Data mining revealed that HLB enhanced transcription of genes involved in the light reactions of photosynthesis and in ATP synthesis. Activation of protein degradation and misfolding processes were observed at the transcriptomic level. Transcripts for heat shock proteins were down-regulated at all disease stages, resulting in further protein misfolding. HLB strongly affected pathways involved in source-sink communication, including sucrose and starch metabolism and hormone synthesis and signaling. Transcription of several genes involved in the synthesis and signal transduction of cytokinins and gibberellins was repressed while that of genes involved in ethylene pathways was induced. CaLas infection triggered a response via both the salicylic acid and jasmonic acid pathways and increased the transcript abundance of several members of the WRKY family of transcription factors. Findings focused on the fruit provide valuable insight to understanding the mechanisms of the HLB-induced fruit disorder and eventually developing methods based on small molecule applications to mitigate its devastating effects on fruit production.

AB - Huanglongbing (HLB) or "citrus greening" is the most destructive citrus disease worldwide. In this work, we studied host responses of citrus to infection with Candidatus Liberibacter asiaticus (CaLas) using next-generation sequencing technologies. A deep mRNA profile was obtained from peel of healthy and HLB- affected fruit. It was followed by pathway and protein-protein network analysis and quantitative real time PCR analysis of highly regulated genes. We identified differentially regulated pathways and constructed networks that provide a deep insight into the metabolism of affected fruit. Data mining revealed that HLB enhanced transcription of genes involved in the light reactions of photosynthesis and in ATP synthesis. Activation of protein degradation and misfolding processes were observed at the transcriptomic level. Transcripts for heat shock proteins were down-regulated at all disease stages, resulting in further protein misfolding. HLB strongly affected pathways involved in source-sink communication, including sucrose and starch metabolism and hormone synthesis and signaling. Transcription of several genes involved in the synthesis and signal transduction of cytokinins and gibberellins was repressed while that of genes involved in ethylene pathways was induced. CaLas infection triggered a response via both the salicylic acid and jasmonic acid pathways and increased the transcript abundance of several members of the WRKY family of transcription factors. Findings focused on the fruit provide valuable insight to understanding the mechanisms of the HLB-induced fruit disorder and eventually developing methods based on small molecule applications to mitigate its devastating effects on fruit production.

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KW - next-generation sequencing

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