Transcriptional analysis of pha genes in pseudomonas mediterranea CFBP 5447 grown on glycerol

Patrizia Bella, Patrizia Bella, Grazia Licciardello, Vittoria Catara, Corinne De Gregorio, Salvatore P. Gugliemino, Vittorio Venturi, Giulia Devescovi

Risultato della ricerca: Articlepeer review

4 Citazioni (Scopus)

Abstract

We analysed the draft genome sequence of Pseudomonas mediterranea CFBP 5447 in order to identify firstly the central metabolic pathways that convert fatty acids or carbohydrate intermediates into mcl-PHA and secondly the genes involved in glycerol metabolism (glpF, glpK, glpD, glpR). Absence of the glpF gene, which codifies for the "glycerol uptake facilitator protein", was highlighted. In order to understand the expression of the pha gene cluster, we investigated the promoter activity of phaC1, phaC2, phaZ, phaD and phaI genes. When glycerol was present as the carbon source, PI was found to be the most active promoter. Expression analysis of the knock-out mutant of the phaD gene, which is a transcriptional regulator belonging to the TetR family, showed that PhaD acts as an activator of the phaI promoter which, in turn, triggers the transcription of the phaIF operon. The activation of PC1, which controls the phaC1ZC2D, by PhaD, was less efficient than PI. © 2014, AIDIC Servizi S.r.l.
Lingua originaleEnglish
pagine (da-a)289-294
Numero di pagine6
RivistaCHEMICAL ENGINEERING TRANSACTIONS
Volume38
Stato di pubblicazionePublished - 2014

All Science Journal Classification (ASJC) codes

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