Trafficking phenotype and production of granzyme B by double negative B cells (IgG(+)IgD(-)CD27(-)) in the elderly.

Bulati M; Buffa S; Martorana A; Candore G; Lio D; Caruso C; Colonna-Romano G

Risultato della ricerca: Article

19 Citazioni (Scopus)

Abstract

The impairment of humoral immune response in elderly humans has been extensively demonstrated. We have reported the increase of memory B cells (IgG+IgD−CD27−, double negative, DN) population in the elderly, in which there is also a typical inflammatory micro-environment. In order to evaluate whether this pro-inflammatory status could influence the trafficking phenotype of naïve/memory B cells, we have assessed the expression of CCR7, CCR6, CXCR3, CXCR4, CXCR5 and CD62L on naïve/memory B cell subpopulations in young and elderly subjects. Moreover, the combination of pro-inflammatory interleukin-21 (IL-21) and B cell receptor (BCR) stimulation enables B cells to produce and secrete granzyme B (GrB), which plays a critical role in early anti-viral immune responses, in the regulation of autoimmune mechanisms and in cancer immunosurveillance. Our data demonstrate that in the elderly, naïve/memory B cell populations present a different expression of the studied receptors that could be discussed in terms of “inflamm-aging”. In particular IgG+IgD−CD27− DN B cells show a tissue trafficking phenotype and they can be stimulated to produce GrB.
Lingua originaleEnglish
pagine (da-a)123-129
Numero di pagine7
RivistaExperimental Gerontology
Volume14
Stato di pubblicazionePublished - 2014

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Immunoglobulin D
Granzymes
B-Lymphocytes
Immunoglobulin G
Cells
Phenotype
Data storage equipment
Immunologic Monitoring
Humoral Immunity
Population
Aging of materials
Tissue

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Cell Biology
  • Endocrinology
  • Genetics
  • Molecular Biology
  • Ageing

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Bulati M; Buffa S; Martorana A; Candore G; Lio D; Caruso C; Colonna-Romano G (2014). Trafficking phenotype and production of granzyme B by double negative B cells (IgG(+)IgD(-)CD27(-)) in the elderly. Experimental Gerontology, 14, 123-129.

Trafficking phenotype and production of granzyme B by double negative B cells (IgG(+)IgD(-)CD27(-)) in the elderly. / Bulati M; Buffa S; Martorana A; Candore G; Lio D; Caruso C; Colonna-Romano G.

In: Experimental Gerontology, Vol. 14, 2014, pag. 123-129.

Risultato della ricerca: Article

Bulati M; Buffa S; Martorana A; Candore G; Lio D; Caruso C; Colonna-Romano G 2014, 'Trafficking phenotype and production of granzyme B by double negative B cells (IgG(+)IgD(-)CD27(-)) in the elderly.', Experimental Gerontology, vol. 14, pagg. 123-129.
Bulati M; Buffa S; Martorana A; Candore G; Lio D; Caruso C; Colonna-Romano G. Trafficking phenotype and production of granzyme B by double negative B cells (IgG(+)IgD(-)CD27(-)) in the elderly. Experimental Gerontology. 2014;14:123-129.
Bulati M; Buffa S; Martorana A; Candore G; Lio D; Caruso C; Colonna-Romano G. / Trafficking phenotype and production of granzyme B by double negative B cells (IgG(+)IgD(-)CD27(-)) in the elderly. In: Experimental Gerontology. 2014 ; Vol. 14. pagg. 123-129.
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title = "Trafficking phenotype and production of granzyme B by double negative B cells (IgG(+)IgD(-)CD27(-)) in the elderly.",
abstract = "The impairment of humoral immune response in elderly humans has been extensively demonstrated. We have reported the increase of memory B cells (IgG+IgD−CD27−, double negative, DN) population in the elderly, in which there is also a typical inflammatory micro-environment. In order to evaluate whether this pro-inflammatory status could influence the trafficking phenotype of na{\"i}ve/memory B cells, we have assessed the expression of CCR7, CCR6, CXCR3, CXCR4, CXCR5 and CD62L on na{\"i}ve/memory B cell subpopulations in young and elderly subjects. Moreover, the combination of pro-inflammatory interleukin-21 (IL-21) and B cell receptor (BCR) stimulation enables B cells to produce and secrete granzyme B (GrB), which plays a critical role in early anti-viral immune responses, in the regulation of autoimmune mechanisms and in cancer immunosurveillance. Our data demonstrate that in the elderly, na{\"i}ve/memory B cell populations present a different expression of the studied receptors that could be discussed in terms of “inflamm-aging”. In particular IgG+IgD−CD27− DN B cells show a tissue trafficking phenotype and they can be stimulated to produce GrB.",
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AU - Bulati M; Buffa S; Martorana A; Candore G; Lio D; Caruso C; Colonna-Romano G

AU - Caruso, Calogero

AU - Colonna Romano, Giuseppina

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AU - Martorana, Adriana

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