Towards the standardization of mitochondrial proteomics: the Italian mt-HPP initiative

Francesca Monteleone, Simona Fontana, Marianna Caterino, Maura Brioschi, Tiziana Alberio, Maurizio Ronci, Alessio Soggiu, Vito Porcelli, Flora Cozzolino, Viviana Greco, Elisa Maffioli, Andrea Urbani, Paola Roncada, Maria Monti, Mara Zilocchi, Valentina Monti, Clizia Chinello, Clara Musicco, Vincenzo Cunsolo, Antonella CormioGiuseppe Petrosillo, Cristina Banfi, Italia Bongarzone, Roberto Scatena, Rosaria Saletti, Luisa Pieroni, Barbara Garavaglia, Mauro Fasano, Laura Giusti, Fulvio Magni, Patrizia Bottoni, Gabriella Tedeschi, Antonio Lucacchini

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18 Citazioni (Scopus)

Abstract

The mitochondrial Human Proteome Project aims at understanding the function of the mitochondrial proteome and its crosstalk with the proteome of other organelles. Being able to choose a suitable and validated enrichment protocol of functional mitochondria, based on the specific needs of the downstream proteomics analysis, would greatly help the researchers in the field. Mitochondrial fractions from ten model cell lines were prepared using three enrichment protocols and analyzed on seven different LC-MS/MS platforms. All data were processed using neXtProt as reference database. The data are available for the Human Proteome Project purposes through the ProteomeXchange Consortium with the identifier PXD007053. The processed datasets were analysed using a suite of R routines to perform a statistical analysis and to retrieve subcellular and sub-mitochondrial localizations. Although the overall number of identified total and mitochondrial proteins was not significantly dependent on the enrichment protocol, specific line to line differences were observed. Moreover, the protein lists were mapped to a network representing the functional mitochondrial proteome, encompassing mitochondrial proteins and their first interactors. More than 80% of the identified proteins resulted nodes of this network but with a different ability in co-isolating mitochondria-associated structures for each enrichment protocol/cell line pair.
Lingua originaleEnglish
pagine (da-a)4319-4329
Numero di pagine11
RivistaJournal of Proteome Research
Volume16
Stato di pubblicazionePublished - 2017

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All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Chemistry(all)

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Monteleone, F., Fontana, S., Caterino, M., Brioschi, M., Alberio, T., Ronci, M., Soggiu, A., Porcelli, V., Cozzolino, F., Greco, V., Maffioli, E., Urbani, A., Roncada, P., Monti, M., Zilocchi, M., Monti, V., Chinello, C., Musicco, C., Cunsolo, V., ... Lucacchini, A. (2017). Towards the standardization of mitochondrial proteomics: the Italian mt-HPP initiative. Journal of Proteome Research, 16, 4319-4329.