Thioflavin T Promotes Aβ(1−40) Amyloid Fibrils Formation

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58 Citazioni (Scopus)

Abstract

Fibrillogenesis of the small peptide Aβ(1−40) is considered to be thehallmark of Alzheimer’s disease. Some evidence indicates small oligomers, rather than mature fibrils, as the key cytotoxic agents. The fluorescent dye Thioflavin T (ThT) is often used to detect amyloid deposits in both in vivo and in vitro experiments, and it is used to study kinetic measurements, under the fundamental hypothesis that this probe does not influence the aggregation processes. We report experimental data showing that ThT maypromote the Aβ(1−40) peptide amyloid aggregation changing solvent−peptide interactions and stabilizing more ordered β-like conformation. This finding has a two-fold importance: It is a fundamental warning in all fibrillation experiments where ThT is used as fluorescent probe, and it suggests that ThT, accelerating fibril formation, could be used to reduce the presence of transient small oligomers, thus interfering with the pathogenic impact of Aβpeptide.
Lingua originaleEnglish
pagine (da-a)1596-1601
Numero di pagine6
RivistaTHE JOURNAL OF PHYSICAL CHEMISTRY LETTERS
Volume3
Stato di pubblicazionePublished - 2012

All Science Journal Classification (ASJC) codes

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  • ???subjectarea.asjc.1600.1606???

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