Thioflavin T Hydroxylation at Basic pH and Its Effect on Amyloid Fibril Detection

Maurizio Leone, Salvatore Spagnolo, Valeria Vetri, Minna Groenning, Vito Foderà, Lars Olsen, Valeria Vetri, Marco Van De Weert, Claus Cornett, Maurizio Leone, Fabio Librizzi

Risultato della ricerca: Articlepeer review

84 Citazioni (Scopus)

Abstract

The fluorescent dye thioflavin T (ThT) is commonly used for in situ amyloid fibril detection. In this work, we focused on the spectroscopic properties and chemical stability of ThT in aqueous solution as a function ofpH, temperature, and dye concentration. A reversible hydroxylation process occurs in alkaline solutions, which was characterized using a combination of UV-vis absorption spectroscopy, proton NMR, and density functionaltheory (DFT). On the basis of these studies, we propose a chemical structure for the hydroxylated form.Finally, by means of fluorescence spectroscopy, ThT hydroxylation effects on in situ amyloid detection have been investigated, providing new insights on the efficiency of the ThT assay for quantitative fibril evaluation at basic pH.
Lingua originaleEnglish
pagine (da-a)15174-15181
Numero di pagine8
RivistaJOURNAL OF PHYSICAL CHEMISTRY. B, CONDENSED MATTER, MATERIALS, SURFACES, INTERFACES & BIOPHYSICAL
Volume112(47)
Stato di pubblicazionePublished - 2008

All Science Journal Classification (ASJC) codes

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  • ???subjectarea.asjc.2500.2508???
  • ???subjectarea.asjc.2500.2505???

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