The transcriptional activator rfiA is quorum-sensing regulated by cotranscription with the luxI homolog pcol and is essential for plant virulence in Pseudomonas corrugata

Patrizia Bella, Patrizia Bella, Laura Steindler, Grazia Licciardello, Vittoria Catara, Iris Bertani, Vittorio Venturi

Risultato della ricerca: Article

14 Citazioni (Scopus)

Abstract

The gram-negative phytopathogen Pseudomonas corrugata has an acyl-homoserine lactone (AHL) quorum-sensing (QS) system called PcoI/PcoR that is involved in virulence on tomato. This work identifies, downstream of pcol, a gene designated rfiA, which we demonstrate is directly linked to QS by cotranscription with pcol. The deduced RfiA protein contains a DNA-binding domain characteristic of the LuxR family but lacks the autoinducer-binding terminus characteristic of the QS LuxR-family proteins. We also identified, downstream of rfiA, an operon designated pcoABC, encoding for the three components of a tripartite resistance nodulation-cell-division (RND) transporter system. The expression of pcoABC is regulated by RfiA. We found that lipodep-sipeptide (LDP) production is cell density dependent and mutants of pcol, pcoR, and rfiA are unable to inhibit the growth of the LDP-sensitive microorganisms Rhodotorula pilimanae and Bacillus megaterium. P. corrugata rfiA mutants were significantly reduced in their ability to cause necrosis development in tomato pith. In addition, it was established that PcoR in the absence of AHL also played a role in virulence on tomato. A model for the role of PcoI, PcoR, and RfiA in tomato pith necrosis is presented. © 2009 The American Phytopathological Society.
Lingua originaleEnglish
pagine (da-a)1514-1522
Numero di pagine9
RivistaMolecular Plant-Microbe Interactions
Volume22
Stato di pubblicazionePublished - 2009

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Pseudomonas corrugata
Quorum Sensing
quorum sensing
Lycopersicon esculentum
Pseudomonas
Virulence
virulence
transcription (genetics)
Acyl-Butyrolactones
tomatoes
homoserine
pith
lactones
necrosis
Necrosis
Rhodotorula
Bacillus megaterium
mutants
Aptitude
DNA-binding domains

All Science Journal Classification (ASJC) codes

  • Physiology
  • Agronomy and Crop Science

Cita questo

The transcriptional activator rfiA is quorum-sensing regulated by cotranscription with the luxI homolog pcol and is essential for plant virulence in Pseudomonas corrugata. / Bella, Patrizia; Bella, Patrizia; Steindler, Laura; Licciardello, Grazia; Catara, Vittoria; Bertani, Iris; Venturi, Vittorio.

In: Molecular Plant-Microbe Interactions, Vol. 22, 2009, pag. 1514-1522.

Risultato della ricerca: Article

Bella, Patrizia ; Bella, Patrizia ; Steindler, Laura ; Licciardello, Grazia ; Catara, Vittoria ; Bertani, Iris ; Venturi, Vittorio. / The transcriptional activator rfiA is quorum-sensing regulated by cotranscription with the luxI homolog pcol and is essential for plant virulence in Pseudomonas corrugata. In: Molecular Plant-Microbe Interactions. 2009 ; Vol. 22. pagg. 1514-1522.
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abstract = "The gram-negative phytopathogen Pseudomonas corrugata has an acyl-homoserine lactone (AHL) quorum-sensing (QS) system called PcoI/PcoR that is involved in virulence on tomato. This work identifies, downstream of pcol, a gene designated rfiA, which we demonstrate is directly linked to QS by cotranscription with pcol. The deduced RfiA protein contains a DNA-binding domain characteristic of the LuxR family but lacks the autoinducer-binding terminus characteristic of the QS LuxR-family proteins. We also identified, downstream of rfiA, an operon designated pcoABC, encoding for the three components of a tripartite resistance nodulation-cell-division (RND) transporter system. The expression of pcoABC is regulated by RfiA. We found that lipodep-sipeptide (LDP) production is cell density dependent and mutants of pcol, pcoR, and rfiA are unable to inhibit the growth of the LDP-sensitive microorganisms Rhodotorula pilimanae and Bacillus megaterium. P. corrugata rfiA mutants were significantly reduced in their ability to cause necrosis development in tomato pith. In addition, it was established that PcoR in the absence of AHL also played a role in virulence on tomato. A model for the role of PcoI, PcoR, and RfiA in tomato pith necrosis is presented. {\^A}{\circledC} 2009 The American Phytopathological Society.",
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T1 - The transcriptional activator rfiA is quorum-sensing regulated by cotranscription with the luxI homolog pcol and is essential for plant virulence in Pseudomonas corrugata

AU - Bella, Patrizia

AU - Bella, Patrizia

AU - Steindler, Laura

AU - Licciardello, Grazia

AU - Catara, Vittoria

AU - Bertani, Iris

AU - Venturi, Vittorio

PY - 2009

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N2 - The gram-negative phytopathogen Pseudomonas corrugata has an acyl-homoserine lactone (AHL) quorum-sensing (QS) system called PcoI/PcoR that is involved in virulence on tomato. This work identifies, downstream of pcol, a gene designated rfiA, which we demonstrate is directly linked to QS by cotranscription with pcol. The deduced RfiA protein contains a DNA-binding domain characteristic of the LuxR family but lacks the autoinducer-binding terminus characteristic of the QS LuxR-family proteins. We also identified, downstream of rfiA, an operon designated pcoABC, encoding for the three components of a tripartite resistance nodulation-cell-division (RND) transporter system. The expression of pcoABC is regulated by RfiA. We found that lipodep-sipeptide (LDP) production is cell density dependent and mutants of pcol, pcoR, and rfiA are unable to inhibit the growth of the LDP-sensitive microorganisms Rhodotorula pilimanae and Bacillus megaterium. P. corrugata rfiA mutants were significantly reduced in their ability to cause necrosis development in tomato pith. In addition, it was established that PcoR in the absence of AHL also played a role in virulence on tomato. A model for the role of PcoI, PcoR, and RfiA in tomato pith necrosis is presented. © 2009 The American Phytopathological Society.

AB - The gram-negative phytopathogen Pseudomonas corrugata has an acyl-homoserine lactone (AHL) quorum-sensing (QS) system called PcoI/PcoR that is involved in virulence on tomato. This work identifies, downstream of pcol, a gene designated rfiA, which we demonstrate is directly linked to QS by cotranscription with pcol. The deduced RfiA protein contains a DNA-binding domain characteristic of the LuxR family but lacks the autoinducer-binding terminus characteristic of the QS LuxR-family proteins. We also identified, downstream of rfiA, an operon designated pcoABC, encoding for the three components of a tripartite resistance nodulation-cell-division (RND) transporter system. The expression of pcoABC is regulated by RfiA. We found that lipodep-sipeptide (LDP) production is cell density dependent and mutants of pcol, pcoR, and rfiA are unable to inhibit the growth of the LDP-sensitive microorganisms Rhodotorula pilimanae and Bacillus megaterium. P. corrugata rfiA mutants were significantly reduced in their ability to cause necrosis development in tomato pith. In addition, it was established that PcoR in the absence of AHL also played a role in virulence on tomato. A model for the role of PcoI, PcoR, and RfiA in tomato pith necrosis is presented. © 2009 The American Phytopathological Society.

UR - http://hdl.handle.net/10447/247636

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