The nucleic acid binding protein PcCNBP is transcriptionally regulated during the immune response in red swamp crayfish Procambarus clarkii

Nicosia, A; Tagliavia, M; Salamone, M; Bennici, C; Masullo, T; Mazzola, S; Gianguzza, F; Cuttitta, A

Risultato della ricerca: Paper

Abstract

Cellular nucleic acid binding proteins (CNBPs) represent a highly conserved protein family among vertebrates; they harbour seven tandem zinc finger repeats CCHC type and have been described as transcriptional and translational regulators. To date, there is little characterization of CNBP in invertebrates since its structure and function have been analysed solely in Drosophila melanogaster. However, no CNBP has been investigated in other arthropod systems. In an effort to isolate immune-related genes in Procambarus clarkii, a partial mRNA coding a zinc finger containing protein was found to be up-regulated during the response to white spot syndrome virus (WSSV) infection. The red swamp crayfish P. clarkii represents an attractive animal model because of its tolerance to extreme environmental conditions and resistance to diseases. Thus it has become an important crustacean model organism for virological studies. In this study, a CNBP homolog from the red swamp crayfish Procambarus clarkii was characterised. The full length cDNA of PcCNBP was of 1257 bp with a 5’-untranslated region (UTR) of 63 bp and a 3’-UTR of 331 bp with a poly (A) tail and an open reading frame (ORF) of 864 bp, encoding a polypeptide of 287 amino acids with the predicted molecular weight of about 33 KDa. The predicted protein possesses 7 tandem repeats of 14 amino acids containing the CCHC zinc finger consensus sequence, two RGG-rich single stranded RNA-binding domain and a Nuclear localization signal, strongly suggesting that PcCNBP was a homolog of vertebrate CNBP. Analyses of transcriptional expression profile showed that PcCNBP was costitutively expressed among different tissues from the adult crayfish, under normal physiological conditions. Moreover, qRT-PCR assays indicate that the transcriptional expression of PcCNBP responds to bacterial and viral stimulation.
Lingua originaleEnglish
Stato di pubblicazionePublished - 2015

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Procambarus clarkii
nucleic acids
binding proteins
immune response
zinc finger motif
vertebrates
nuclear localization signals
White spot syndrome virus
amino acids
tandem repeat sequences
proteins
consensus sequence
5' untranslated regions
3' untranslated regions
messenger RNA
crayfish
Drosophila melanogaster
open reading frames
arthropods
polypeptides

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Nicosia, A; Tagliavia, M; Salamone, M; Bennici, C; Masullo, T; Mazzola, S; Gianguzza, F; Cuttitta, A (2015). The nucleic acid binding protein PcCNBP is transcriptionally regulated during the immune response in red swamp crayfish Procambarus clarkii.

The nucleic acid binding protein PcCNBP is transcriptionally regulated during the immune response in red swamp crayfish Procambarus clarkii. / Nicosia, A; Tagliavia, M; Salamone, M; Bennici, C; Masullo, T; Mazzola, S; Gianguzza, F; Cuttitta, A.

2015.

Risultato della ricerca: Paper

Nicosia, A; Tagliavia, M; Salamone, M; Bennici, C; Masullo, T; Mazzola, S; Gianguzza, F; Cuttitta, A 2015, 'The nucleic acid binding protein PcCNBP is transcriptionally regulated during the immune response in red swamp crayfish Procambarus clarkii'.
Nicosia, A; Tagliavia, M; Salamone, M; Bennici, C; Masullo, T; Mazzola, S; Gianguzza, F; Cuttitta, A. The nucleic acid binding protein PcCNBP is transcriptionally regulated during the immune response in red swamp crayfish Procambarus clarkii. 2015.
Nicosia, A; Tagliavia, M; Salamone, M; Bennici, C; Masullo, T; Mazzola, S; Gianguzza, F; Cuttitta, A. / The nucleic acid binding protein PcCNBP is transcriptionally regulated during the immune response in red swamp crayfish Procambarus clarkii.
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title = "The nucleic acid binding protein PcCNBP is transcriptionally regulated during the immune response in red swamp crayfish Procambarus clarkii",
abstract = "Cellular nucleic acid binding proteins (CNBPs) represent a highly conserved protein family among vertebrates; they harbour seven tandem zinc finger repeats CCHC type and have been described as transcriptional and translational regulators. To date, there is little characterization of CNBP in invertebrates since its structure and function have been analysed solely in Drosophila melanogaster. However, no CNBP has been investigated in other arthropod systems. In an effort to isolate immune-related genes in Procambarus clarkii, a partial mRNA coding a zinc finger containing protein was found to be up-regulated during the response to white spot syndrome virus (WSSV) infection. The red swamp crayfish P. clarkii represents an attractive animal model because of its tolerance to extreme environmental conditions and resistance to diseases. Thus it has become an important crustacean model organism for virological studies. In this study, a CNBP homolog from the red swamp crayfish Procambarus clarkii was characterised. The full length cDNA of PcCNBP was of 1257 bp with a 5’-untranslated region (UTR) of 63 bp and a 3’-UTR of 331 bp with a poly (A) tail and an open reading frame (ORF) of 864 bp, encoding a polypeptide of 287 amino acids with the predicted molecular weight of about 33 KDa. The predicted protein possesses 7 tandem repeats of 14 amino acids containing the CCHC zinc finger consensus sequence, two RGG-rich single stranded RNA-binding domain and a Nuclear localization signal, strongly suggesting that PcCNBP was a homolog of vertebrate CNBP. Analyses of transcriptional expression profile showed that PcCNBP was costitutively expressed among different tissues from the adult crayfish, under normal physiological conditions. Moreover, qRT-PCR assays indicate that the transcriptional expression of PcCNBP responds to bacterial and viral stimulation.",
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T1 - The nucleic acid binding protein PcCNBP is transcriptionally regulated during the immune response in red swamp crayfish Procambarus clarkii

AU - Nicosia, A; Tagliavia, M; Salamone, M; Bennici, C; Masullo, T; Mazzola, S; Gianguzza, F; Cuttitta, A

AU - Ragusa, Maria Antonietta

AU - Costa, Salvatore

PY - 2015

Y1 - 2015

N2 - Cellular nucleic acid binding proteins (CNBPs) represent a highly conserved protein family among vertebrates; they harbour seven tandem zinc finger repeats CCHC type and have been described as transcriptional and translational regulators. To date, there is little characterization of CNBP in invertebrates since its structure and function have been analysed solely in Drosophila melanogaster. However, no CNBP has been investigated in other arthropod systems. In an effort to isolate immune-related genes in Procambarus clarkii, a partial mRNA coding a zinc finger containing protein was found to be up-regulated during the response to white spot syndrome virus (WSSV) infection. The red swamp crayfish P. clarkii represents an attractive animal model because of its tolerance to extreme environmental conditions and resistance to diseases. Thus it has become an important crustacean model organism for virological studies. In this study, a CNBP homolog from the red swamp crayfish Procambarus clarkii was characterised. The full length cDNA of PcCNBP was of 1257 bp with a 5’-untranslated region (UTR) of 63 bp and a 3’-UTR of 331 bp with a poly (A) tail and an open reading frame (ORF) of 864 bp, encoding a polypeptide of 287 amino acids with the predicted molecular weight of about 33 KDa. The predicted protein possesses 7 tandem repeats of 14 amino acids containing the CCHC zinc finger consensus sequence, two RGG-rich single stranded RNA-binding domain and a Nuclear localization signal, strongly suggesting that PcCNBP was a homolog of vertebrate CNBP. Analyses of transcriptional expression profile showed that PcCNBP was costitutively expressed among different tissues from the adult crayfish, under normal physiological conditions. Moreover, qRT-PCR assays indicate that the transcriptional expression of PcCNBP responds to bacterial and viral stimulation.

AB - Cellular nucleic acid binding proteins (CNBPs) represent a highly conserved protein family among vertebrates; they harbour seven tandem zinc finger repeats CCHC type and have been described as transcriptional and translational regulators. To date, there is little characterization of CNBP in invertebrates since its structure and function have been analysed solely in Drosophila melanogaster. However, no CNBP has been investigated in other arthropod systems. In an effort to isolate immune-related genes in Procambarus clarkii, a partial mRNA coding a zinc finger containing protein was found to be up-regulated during the response to white spot syndrome virus (WSSV) infection. The red swamp crayfish P. clarkii represents an attractive animal model because of its tolerance to extreme environmental conditions and resistance to diseases. Thus it has become an important crustacean model organism for virological studies. In this study, a CNBP homolog from the red swamp crayfish Procambarus clarkii was characterised. The full length cDNA of PcCNBP was of 1257 bp with a 5’-untranslated region (UTR) of 63 bp and a 3’-UTR of 331 bp with a poly (A) tail and an open reading frame (ORF) of 864 bp, encoding a polypeptide of 287 amino acids with the predicted molecular weight of about 33 KDa. The predicted protein possesses 7 tandem repeats of 14 amino acids containing the CCHC zinc finger consensus sequence, two RGG-rich single stranded RNA-binding domain and a Nuclear localization signal, strongly suggesting that PcCNBP was a homolog of vertebrate CNBP. Analyses of transcriptional expression profile showed that PcCNBP was costitutively expressed among different tissues from the adult crayfish, under normal physiological conditions. Moreover, qRT-PCR assays indicate that the transcriptional expression of PcCNBP responds to bacterial and viral stimulation.

UR - http://hdl.handle.net/10447/235433

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