The major allergen of the Parietaria pollen contains anLPS-binding region with immuno-modulatory activity

Fabrizio Gianguzza, Paolo Colombo, Schiavi, Giacomazza, Corinti, Angela Bonura, Di Felice

Risultato della ricerca: Articlepeer review

11 Citazioni (Scopus)

Abstract

Background: The major allergens in Parietaria pollen, Par j 1 and Par j 2, have been identified as lipid transfer proteins. The family of the Par j 1 allergens is composed of two isoforms, which differ by the presence of a 37 amino acid peptide (Par37) exclusive to the Par j 1.0101 isoform. The goal of this study was to elucidate the biological properties of the Par37 peptide.Methods: In silico analysis, spectrofluorimetric experiments and in vitro cell culture assays were used to identify the biological properties of Par37. In addition, amouse model of sensitization was used to study the influence of Par37 in the murine immune response.Results: In silico analysis predicted that Par37 displays characteristics of a host defence peptide. Spectrofluorimetric analysis, real-time PCR and ELISA assaysdemonstrated that Par37 possesses an LPS-binding activity influencing cell signalling in vitro. In RAW264.7 cells, LPS-induced IL-6 and TNF-a transcription andtranslation were inhibited after preincubation with Par37. Consistent with these data, inhibition of IFN-c secretion was observed in murine spleen cells and in human PBMC. Finally, mice immunized with the two Par j 1 isoforms differing in the presence or absence of the Par37 peptide showed different immunological behaviours in vivo.Conclusions: This study demonstrates that the Par j 1.0101 allergen displays LPSbinding activity due to the presence of a 37 amino acid COOH-terminal regionand that this region is capable of influencing cytokine and antibody responses in vitro and in vivo.
Lingua originaleEnglish
Numero di pagine0
RivistaALLERGY
Volumeonline
Stato di pubblicazionePublished - 2013

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

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