The increase in maternal expression of axin1 and axin2 contribute to the zebrafish mutant ichabod ventralized phenotype.

Renza Vento, Claudia Covaciu, Yuko Komiya, Valeria Rizzo, Jessica Ibetti, Marcella Macaluso, Melissa Baxter, Lauren Derstine, Fabio Valenti, Giuseppe Russo, Gianfranco Bellipanni, Raymond Habas, Daniele Castiglia, Cara J. Gottardi, Anna Maria Lucchese, Franco Cotelli, Antonio Giordano, Giuseppe Russo

Risultato della ricerca: Article

4 Citazioni (Scopus)

Abstract

β-catenin is a central effector of the Wnt pathway and one of the players in Ca+ -dependent cell-cell adhesion. While many wnts are present and expressed in vertebrates, only one β-catenin exists in the majority of the organisms. One intriguing exception is zebrafish that carries two genes for β-catenin. The maternal recessive mutation ichabod presents very low levels of β-catenin2 that in turn affects dorsal axis formation, suggesting that β-catenin1 is incapable to compensate for β-catenin2 loss and raising the question of whether these two β-catenins may have differential roles during early axis specification. Here we identify a specific antibody that can discriminate selectively for β-catenin1. By confocal co-immunofluorescent analysis and low concentration gain-of-function experiments, we show that β-catenin1 and 2 behave in similar modes in dorsal axis induction and cellular localization. Surprisingly, we also found that in the ich embryo the mRNAs of the components of β-catenin regulatory pathway, including β-catenin1, are more abundant than in the Wt embryo. Increased levels of β-catenin1 are found at the membrane level but not in the nuclei till high stage. Finally, we present evidence that β-catenin1 cannot revert the ich phenotype because it may be under the control of a GSK3β-independent mechanism that required Axin's RGS domain function.
Lingua originaleEnglish
pagine (da-a)418-430
Numero di pagine13
RivistaJournal of Cellular Biochemistry
Volume116
Stato di pubblicazionePublished - 2015

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Catenins
Zebrafish
Mothers
Phenotype
Embryonic Structures
Wnt Signaling Pathway
Cell adhesion
Cell Adhesion
Vertebrates
Genes
Membranes
Specifications
Messenger RNA
Mutation
Antibodies
Experiments

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cita questo

The increase in maternal expression of axin1 and axin2 contribute to the zebrafish mutant ichabod ventralized phenotype. / Vento, Renza; Covaciu, Claudia; Komiya, Yuko; Rizzo, Valeria; Ibetti, Jessica; Macaluso, Marcella; Baxter, Melissa; Derstine, Lauren; Valenti, Fabio; Russo, Giuseppe; Bellipanni, Gianfranco; Habas, Raymond; Castiglia, Daniele; Gottardi, Cara J.; Lucchese, Anna Maria; Cotelli, Franco; Giordano, Antonio; Russo, Giuseppe.

In: Journal of Cellular Biochemistry, Vol. 116, 2015, pag. 418-430.

Risultato della ricerca: Article

Vento, R, Covaciu, C, Komiya, Y, Rizzo, V, Ibetti, J, Macaluso, M, Baxter, M, Derstine, L, Valenti, F, Russo, G, Bellipanni, G, Habas, R, Castiglia, D, Gottardi, CJ, Lucchese, AM, Cotelli, F, Giordano, A & Russo, G 2015, 'The increase in maternal expression of axin1 and axin2 contribute to the zebrafish mutant ichabod ventralized phenotype.', Journal of Cellular Biochemistry, vol. 116, pagg. 418-430.
Vento, Renza ; Covaciu, Claudia ; Komiya, Yuko ; Rizzo, Valeria ; Ibetti, Jessica ; Macaluso, Marcella ; Baxter, Melissa ; Derstine, Lauren ; Valenti, Fabio ; Russo, Giuseppe ; Bellipanni, Gianfranco ; Habas, Raymond ; Castiglia, Daniele ; Gottardi, Cara J. ; Lucchese, Anna Maria ; Cotelli, Franco ; Giordano, Antonio ; Russo, Giuseppe. / The increase in maternal expression of axin1 and axin2 contribute to the zebrafish mutant ichabod ventralized phenotype. In: Journal of Cellular Biochemistry. 2015 ; Vol. 116. pagg. 418-430.
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abstract = "β-catenin is a central effector of the Wnt pathway and one of the players in Ca+ -dependent cell-cell adhesion. While many wnts are present and expressed in vertebrates, only one β-catenin exists in the majority of the organisms. One intriguing exception is zebrafish that carries two genes for β-catenin. The maternal recessive mutation ichabod presents very low levels of β-catenin2 that in turn affects dorsal axis formation, suggesting that β-catenin1 is incapable to compensate for β-catenin2 loss and raising the question of whether these two β-catenins may have differential roles during early axis specification. Here we identify a specific antibody that can discriminate selectively for β-catenin1. By confocal co-immunofluorescent analysis and low concentration gain-of-function experiments, we show that β-catenin1 and 2 behave in similar modes in dorsal axis induction and cellular localization. Surprisingly, we also found that in the ich embryo the mRNAs of the components of β-catenin regulatory pathway, including β-catenin1, are more abundant than in the Wt embryo. Increased levels of β-catenin1 are found at the membrane level but not in the nuclei till high stage. Finally, we present evidence that β-catenin1 cannot revert the ich phenotype because it may be under the control of a GSK3β-independent mechanism that required Axin's RGS domain function.",
author = "Renza Vento and Claudia Covaciu and Yuko Komiya and Valeria Rizzo and Jessica Ibetti and Marcella Macaluso and Melissa Baxter and Lauren Derstine and Fabio Valenti and Giuseppe Russo and Gianfranco Bellipanni and Raymond Habas and Daniele Castiglia and Gottardi, {Cara J.} and Lucchese, {Anna Maria} and Franco Cotelli and Antonio Giordano and Giuseppe Russo",
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T1 - The increase in maternal expression of axin1 and axin2 contribute to the zebrafish mutant ichabod ventralized phenotype.

AU - Vento, Renza

AU - Covaciu, Claudia

AU - Komiya, Yuko

AU - Rizzo, Valeria

AU - Ibetti, Jessica

AU - Macaluso, Marcella

AU - Baxter, Melissa

AU - Derstine, Lauren

AU - Valenti, Fabio

AU - Russo, Giuseppe

AU - Bellipanni, Gianfranco

AU - Habas, Raymond

AU - Castiglia, Daniele

AU - Gottardi, Cara J.

AU - Lucchese, Anna Maria

AU - Cotelli, Franco

AU - Giordano, Antonio

AU - Russo, Giuseppe

PY - 2015

Y1 - 2015

N2 - β-catenin is a central effector of the Wnt pathway and one of the players in Ca+ -dependent cell-cell adhesion. While many wnts are present and expressed in vertebrates, only one β-catenin exists in the majority of the organisms. One intriguing exception is zebrafish that carries two genes for β-catenin. The maternal recessive mutation ichabod presents very low levels of β-catenin2 that in turn affects dorsal axis formation, suggesting that β-catenin1 is incapable to compensate for β-catenin2 loss and raising the question of whether these two β-catenins may have differential roles during early axis specification. Here we identify a specific antibody that can discriminate selectively for β-catenin1. By confocal co-immunofluorescent analysis and low concentration gain-of-function experiments, we show that β-catenin1 and 2 behave in similar modes in dorsal axis induction and cellular localization. Surprisingly, we also found that in the ich embryo the mRNAs of the components of β-catenin regulatory pathway, including β-catenin1, are more abundant than in the Wt embryo. Increased levels of β-catenin1 are found at the membrane level but not in the nuclei till high stage. Finally, we present evidence that β-catenin1 cannot revert the ich phenotype because it may be under the control of a GSK3β-independent mechanism that required Axin's RGS domain function.

AB - β-catenin is a central effector of the Wnt pathway and one of the players in Ca+ -dependent cell-cell adhesion. While many wnts are present and expressed in vertebrates, only one β-catenin exists in the majority of the organisms. One intriguing exception is zebrafish that carries two genes for β-catenin. The maternal recessive mutation ichabod presents very low levels of β-catenin2 that in turn affects dorsal axis formation, suggesting that β-catenin1 is incapable to compensate for β-catenin2 loss and raising the question of whether these two β-catenins may have differential roles during early axis specification. Here we identify a specific antibody that can discriminate selectively for β-catenin1. By confocal co-immunofluorescent analysis and low concentration gain-of-function experiments, we show that β-catenin1 and 2 behave in similar modes in dorsal axis induction and cellular localization. Surprisingly, we also found that in the ich embryo the mRNAs of the components of β-catenin regulatory pathway, including β-catenin1, are more abundant than in the Wt embryo. Increased levels of β-catenin1 are found at the membrane level but not in the nuclei till high stage. Finally, we present evidence that β-catenin1 cannot revert the ich phenotype because it may be under the control of a GSK3β-independent mechanism that required Axin's RGS domain function.

UR - http://hdl.handle.net/10447/109413

M3 - Article

VL - 116

SP - 418

EP - 430

JO - Journal of Cellular Biochemistry

JF - Journal of Cellular Biochemistry

SN - 0730-2312

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