The identification and localization of two intermediate filament proteinsin the tunic of Styela plicata (Tunicata, Styelidae)

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Abstract

The intermediate filament (IF) proteins Styela C and Styela D from the tunicate Styela (Urochordata) are co-expressed in all epidermal cells and they are thought to behave as type I and type II keratins. These two IF proteins, Styela C and Styela D, were identified in immunoblots of proteins isolated from the tunic of Styela plicata. The occurrence and distribution of these proteins within the tunic of this ascidian was examined by means of immunofluorescence and immunoperoxidase techniques, using anti-Styela C and anti-Styela D antibodies. In addition, immuno-electron microscopy of the tunic showed that the two proteins are located in the cuticle layer and in the tunic matrix. These results represent the first data about the presence of IF proteins in the tunic of adult ascidian S. plicata. The possible involvement of these IF proteins in reinforcing the integrity of the tunic, that represents the interface between the animal body and the external environment, is discussed.
Lingua originaleEnglish
Numero di pagine9
RivistaTISSUE & CELL
Volume2009
Stato di pubblicazionePublished - 2009

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Intermediate Filament Proteins
Urochordata
Intermediate Filaments
Protein C
Type I Keratin
Type II Keratin
Proteins
Immunoelectron Microscopy
Immunoenzyme Techniques
Fluorescent Antibody Technique
Antibodies

All Science Journal Classification (ASJC) codes

  • Developmental Biology
  • Cell Biology

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title = "The identification and localization of two intermediate filament proteinsin the tunic of Styela plicata (Tunicata, Styelidae)",
abstract = "The intermediate filament (IF) proteins Styela C and Styela D from the tunicate Styela (Urochordata) are co-expressed in all epidermal cells and they are thought to behave as type I and type II keratins. These two IF proteins, Styela C and Styela D, were identified in immunoblots of proteins isolated from the tunic of Styela plicata. The occurrence and distribution of these proteins within the tunic of this ascidian was examined by means of immunofluorescence and immunoperoxidase techniques, using anti-Styela C and anti-Styela D antibodies. In addition, immuno-electron microscopy of the tunic showed that the two proteins are located in the cuticle layer and in the tunic matrix. These results represent the first data about the presence of IF proteins in the tunic of adult ascidian S. plicata. The possible involvement of these IF proteins in reinforcing the integrity of the tunic, that represents the interface between the animal body and the external environment, is discussed.",
keywords = "Styela plicata, Tunicata, immunolocalization, intermediate filaments",
author = "Riccardo Alessandro and {Di Bella}, {Maria Antonietta} and {De Leo}, Giacomo",
year = "2009",
language = "English",
volume = "2009",
journal = "TISSUE & CELL",
issn = "0040-8166",

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TY - JOUR

T1 - The identification and localization of two intermediate filament proteinsin the tunic of Styela plicata (Tunicata, Styelidae)

AU - Alessandro, Riccardo

AU - Di Bella, Maria Antonietta

AU - De Leo, Giacomo

PY - 2009

Y1 - 2009

N2 - The intermediate filament (IF) proteins Styela C and Styela D from the tunicate Styela (Urochordata) are co-expressed in all epidermal cells and they are thought to behave as type I and type II keratins. These two IF proteins, Styela C and Styela D, were identified in immunoblots of proteins isolated from the tunic of Styela plicata. The occurrence and distribution of these proteins within the tunic of this ascidian was examined by means of immunofluorescence and immunoperoxidase techniques, using anti-Styela C and anti-Styela D antibodies. In addition, immuno-electron microscopy of the tunic showed that the two proteins are located in the cuticle layer and in the tunic matrix. These results represent the first data about the presence of IF proteins in the tunic of adult ascidian S. plicata. The possible involvement of these IF proteins in reinforcing the integrity of the tunic, that represents the interface between the animal body and the external environment, is discussed.

AB - The intermediate filament (IF) proteins Styela C and Styela D from the tunicate Styela (Urochordata) are co-expressed in all epidermal cells and they are thought to behave as type I and type II keratins. These two IF proteins, Styela C and Styela D, were identified in immunoblots of proteins isolated from the tunic of Styela plicata. The occurrence and distribution of these proteins within the tunic of this ascidian was examined by means of immunofluorescence and immunoperoxidase techniques, using anti-Styela C and anti-Styela D antibodies. In addition, immuno-electron microscopy of the tunic showed that the two proteins are located in the cuticle layer and in the tunic matrix. These results represent the first data about the presence of IF proteins in the tunic of adult ascidian S. plicata. The possible involvement of these IF proteins in reinforcing the integrity of the tunic, that represents the interface between the animal body and the external environment, is discussed.

KW - Styela plicata

KW - Tunicata

KW - immunolocalization

KW - intermediate filaments

UR - http://hdl.handle.net/10447/37963

M3 - Article

VL - 2009

JO - TISSUE & CELL

JF - TISSUE & CELL

SN - 0040-8166

ER -