The gelatinase MMP-9like is involved in regulation of LPS inflammatoryresponse in Ciona robusta

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Matrix metalloproteinases (MMPs) are a family of endopeptidases collectively able to degrade the components ofthe extracellular matrix (ECM), with important roles in many biological processes, such as embryogenesis,normal tissue remodelling, angiogenesis and wound healing. New views on the function of MMPs reveal thatthey regulate inflammatory response and therefore might represent an early step in the evolution of the immunesystem. MMPs can affect the activity of cytokines involved in inflammation including TGF-β and TNF-α. MMPsare widely distributed in all kingdoms of life and have likely evolved from a single-domain protein whichunderwent successive rounds of duplications. In this study, we focused on the Ciona robusta (formerly known asCiona intestinalis) MMP gelatinase homologue. Gene organization, phylogenetic analysis and 3D modeling supportedthe closest correlation of C. robusta gelatinase with the human MMP-9. Real-time PCR analysis andzymographic assay showed a prompt expression induced by LPS inoculation and an upregulation of enzymaticactivity. Furthermore, we showed that before of the well-known increase of TGF-β and TNF-α levels, a MMP-9like boost occurred, suggesting a possible involvement of MMP-9like in regulating inflammatory response in C.robusta.
Lingua originaleEnglish
pagine (da-a)213-222
Numero di pagine10
Stato di pubblicazionePublished - 2019

All Science Journal Classification (ASJC) codes

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  • ???subjectarea.asjc.1100.1104???


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