The expression of HSP60 and HSP10 in large bowel carcinomas with lymph node metastase

Giovanni Zummo, Sabrina David, Felicia Farina, Francesca Rappa, Fabio Bucchieri, Francesco Cappello, Francesca Rappa, Lorenzo Marasà, Francesco Cappello, Sabrina David, Tommaso E. Bartolotta, Lorenzo Marasa'

Risultato della ricerca: Article

96 Citazioni (Scopus)

Abstract

Background: The involvement of Heat Shock Proteins (HSP) in cancer development and progression is a widely debated topic. The objective of the present study was to evaluate the presence and expression of HSP60 and HSP10 in a series of large bowel carcinomas and locoregional lymph nodes with and without metastases. Methods: 82 Astler and Coller's stage C2 colorectal cancers, of which 48 well-differentiated and 34 poorlydifferentiated, were selected along with 661 lymph nodes, including 372 with metastases and 289 with reactive hyperplasia only, from the same tumours. Primitive tumours and both metastatic and reactive lymph nodes were studied; specifically, three different compartments of the lymph nodes, secondary follicle, paracortex and medullary sinus, were also analysed. An immunohistochemical research for HSP60 and HSP10 was performed and the semiquantitative results were analysed by statistical analysis to determine the correlation between HSPs expression and 1) tumour grading; 2) degree of inflammation; 3) number of lymph nodes involved; 4) lymph node compartment hyperplasia. Moreover, western blotting was performed on a smaller group of samples to confirm the immunohistochemical results.Results: Our data show that the expression of HSP60, in both primary tumour and lymph node metastasis, is correlated with the tumoral grade, while the HSP10 expression is not. Nevertheless, the levels of HSP10 are commonly higher than the levels of HSP60. In addition, statistical analyses do not show any correlation between the degree of inflammation and the immunopositivity for both HSP60 and HSP10. Moreover, we find a significant correlation between the presence of lymph node metastases and the positivity for both HSP60 and HSP10. In particular, metastatic lymph nodes show a higher percentage of cells positive for both HSP60 and HSP10 in the secondary follicles, and for HSP10 in the medullary sinuses, when compared with hyperplastic lymph nodes. Conclusion: HSP60 and HSP10 may have diagnostic and prognostic significance in the management of this tumour and their overexpression in tumoral cells may be functionally related to tumoral progression. We hypothesise that their expression in follicular and medullary cells of lymph nodes may be induced by formation of metastases. Further studies based on these observations could lead to a better understanding of the HSPs involvement in colorectal cancer progression, as well as other neoplasms.
Lingua originaleEnglish
pagine (da-a)139-139
Numero di pagine10
RivistaBMC Cancer
Volume5
Stato di pubblicazionePublished - 2005

Fingerprint

Lymph Nodes
Carcinoma
Neoplasm Metastasis
Neoplasms
Hyperplasia
Colorectal Neoplasms
Inflammation
Neoplasm Grading
Heat-Shock Proteins
Western Blotting
Research

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research
  • Genetics

Cita questo

The expression of HSP60 and HSP10 in large bowel carcinomas with lymph node metastase. / Zummo, Giovanni; David, Sabrina; Farina, Felicia; Rappa, Francesca; Bucchieri, Fabio; Cappello, Francesco; Rappa, Francesca; Marasà, Lorenzo; Cappello, Francesco; David, Sabrina; Bartolotta, Tommaso E.; Marasa', Lorenzo.

In: BMC Cancer, Vol. 5, 2005, pag. 139-139.

Risultato della ricerca: Article

Zummo, G, David, S, Farina, F, Rappa, F, Bucchieri, F, Cappello, F, Rappa, F, Marasà, L, Cappello, F, David, S, Bartolotta, TE & Marasa', L 2005, 'The expression of HSP60 and HSP10 in large bowel carcinomas with lymph node metastase', BMC Cancer, vol. 5, pagg. 139-139.
Zummo, Giovanni ; David, Sabrina ; Farina, Felicia ; Rappa, Francesca ; Bucchieri, Fabio ; Cappello, Francesco ; Rappa, Francesca ; Marasà, Lorenzo ; Cappello, Francesco ; David, Sabrina ; Bartolotta, Tommaso E. ; Marasa', Lorenzo. / The expression of HSP60 and HSP10 in large bowel carcinomas with lymph node metastase. In: BMC Cancer. 2005 ; Vol. 5. pagg. 139-139.
@article{bb9a1123f7624feeb164f58e35b6570c,
title = "The expression of HSP60 and HSP10 in large bowel carcinomas with lymph node metastase",
abstract = "Background: The involvement of Heat Shock Proteins (HSP) in cancer development and progression is a widely debated topic. The objective of the present study was to evaluate the presence and expression of HSP60 and HSP10 in a series of large bowel carcinomas and locoregional lymph nodes with and without metastases. Methods: 82 Astler and Coller's stage C2 colorectal cancers, of which 48 well-differentiated and 34 poorlydifferentiated, were selected along with 661 lymph nodes, including 372 with metastases and 289 with reactive hyperplasia only, from the same tumours. Primitive tumours and both metastatic and reactive lymph nodes were studied; specifically, three different compartments of the lymph nodes, secondary follicle, paracortex and medullary sinus, were also analysed. An immunohistochemical research for HSP60 and HSP10 was performed and the semiquantitative results were analysed by statistical analysis to determine the correlation between HSPs expression and 1) tumour grading; 2) degree of inflammation; 3) number of lymph nodes involved; 4) lymph node compartment hyperplasia. Moreover, western blotting was performed on a smaller group of samples to confirm the immunohistochemical results.Results: Our data show that the expression of HSP60, in both primary tumour and lymph node metastasis, is correlated with the tumoral grade, while the HSP10 expression is not. Nevertheless, the levels of HSP10 are commonly higher than the levels of HSP60. In addition, statistical analyses do not show any correlation between the degree of inflammation and the immunopositivity for both HSP60 and HSP10. Moreover, we find a significant correlation between the presence of lymph node metastases and the positivity for both HSP60 and HSP10. In particular, metastatic lymph nodes show a higher percentage of cells positive for both HSP60 and HSP10 in the secondary follicles, and for HSP10 in the medullary sinuses, when compared with hyperplastic lymph nodes. Conclusion: HSP60 and HSP10 may have diagnostic and prognostic significance in the management of this tumour and their overexpression in tumoral cells may be functionally related to tumoral progression. We hypothesise that their expression in follicular and medullary cells of lymph nodes may be induced by formation of metastases. Further studies based on these observations could lead to a better understanding of the HSPs involvement in colorectal cancer progression, as well as other neoplasms.",
author = "Giovanni Zummo and Sabrina David and Felicia Farina and Francesca Rappa and Fabio Bucchieri and Francesco Cappello and Francesca Rappa and Lorenzo Maras{\`a} and Francesco Cappello and Sabrina David and Bartolotta, {Tommaso E.} and Lorenzo Marasa'",
year = "2005",
language = "English",
volume = "5",
pages = "139--139",
journal = "BMC Cancer",
issn = "1471-2407",
publisher = "BioMed Central",

}

TY - JOUR

T1 - The expression of HSP60 and HSP10 in large bowel carcinomas with lymph node metastase

AU - Zummo, Giovanni

AU - David, Sabrina

AU - Farina, Felicia

AU - Rappa, Francesca

AU - Bucchieri, Fabio

AU - Cappello, Francesco

AU - Rappa, Francesca

AU - Marasà, Lorenzo

AU - Cappello, Francesco

AU - David, Sabrina

AU - Bartolotta, Tommaso E.

AU - Marasa', Lorenzo

PY - 2005

Y1 - 2005

N2 - Background: The involvement of Heat Shock Proteins (HSP) in cancer development and progression is a widely debated topic. The objective of the present study was to evaluate the presence and expression of HSP60 and HSP10 in a series of large bowel carcinomas and locoregional lymph nodes with and without metastases. Methods: 82 Astler and Coller's stage C2 colorectal cancers, of which 48 well-differentiated and 34 poorlydifferentiated, were selected along with 661 lymph nodes, including 372 with metastases and 289 with reactive hyperplasia only, from the same tumours. Primitive tumours and both metastatic and reactive lymph nodes were studied; specifically, three different compartments of the lymph nodes, secondary follicle, paracortex and medullary sinus, were also analysed. An immunohistochemical research for HSP60 and HSP10 was performed and the semiquantitative results were analysed by statistical analysis to determine the correlation between HSPs expression and 1) tumour grading; 2) degree of inflammation; 3) number of lymph nodes involved; 4) lymph node compartment hyperplasia. Moreover, western blotting was performed on a smaller group of samples to confirm the immunohistochemical results.Results: Our data show that the expression of HSP60, in both primary tumour and lymph node metastasis, is correlated with the tumoral grade, while the HSP10 expression is not. Nevertheless, the levels of HSP10 are commonly higher than the levels of HSP60. In addition, statistical analyses do not show any correlation between the degree of inflammation and the immunopositivity for both HSP60 and HSP10. Moreover, we find a significant correlation between the presence of lymph node metastases and the positivity for both HSP60 and HSP10. In particular, metastatic lymph nodes show a higher percentage of cells positive for both HSP60 and HSP10 in the secondary follicles, and for HSP10 in the medullary sinuses, when compared with hyperplastic lymph nodes. Conclusion: HSP60 and HSP10 may have diagnostic and prognostic significance in the management of this tumour and their overexpression in tumoral cells may be functionally related to tumoral progression. We hypothesise that their expression in follicular and medullary cells of lymph nodes may be induced by formation of metastases. Further studies based on these observations could lead to a better understanding of the HSPs involvement in colorectal cancer progression, as well as other neoplasms.

AB - Background: The involvement of Heat Shock Proteins (HSP) in cancer development and progression is a widely debated topic. The objective of the present study was to evaluate the presence and expression of HSP60 and HSP10 in a series of large bowel carcinomas and locoregional lymph nodes with and without metastases. Methods: 82 Astler and Coller's stage C2 colorectal cancers, of which 48 well-differentiated and 34 poorlydifferentiated, were selected along with 661 lymph nodes, including 372 with metastases and 289 with reactive hyperplasia only, from the same tumours. Primitive tumours and both metastatic and reactive lymph nodes were studied; specifically, three different compartments of the lymph nodes, secondary follicle, paracortex and medullary sinus, were also analysed. An immunohistochemical research for HSP60 and HSP10 was performed and the semiquantitative results were analysed by statistical analysis to determine the correlation between HSPs expression and 1) tumour grading; 2) degree of inflammation; 3) number of lymph nodes involved; 4) lymph node compartment hyperplasia. Moreover, western blotting was performed on a smaller group of samples to confirm the immunohistochemical results.Results: Our data show that the expression of HSP60, in both primary tumour and lymph node metastasis, is correlated with the tumoral grade, while the HSP10 expression is not. Nevertheless, the levels of HSP10 are commonly higher than the levels of HSP60. In addition, statistical analyses do not show any correlation between the degree of inflammation and the immunopositivity for both HSP60 and HSP10. Moreover, we find a significant correlation between the presence of lymph node metastases and the positivity for both HSP60 and HSP10. In particular, metastatic lymph nodes show a higher percentage of cells positive for both HSP60 and HSP10 in the secondary follicles, and for HSP10 in the medullary sinuses, when compared with hyperplastic lymph nodes. Conclusion: HSP60 and HSP10 may have diagnostic and prognostic significance in the management of this tumour and their overexpression in tumoral cells may be functionally related to tumoral progression. We hypothesise that their expression in follicular and medullary cells of lymph nodes may be induced by formation of metastases. Further studies based on these observations could lead to a better understanding of the HSPs involvement in colorectal cancer progression, as well as other neoplasms.

UR - http://hdl.handle.net/10447/11007

M3 - Article

VL - 5

SP - 139

EP - 139

JO - BMC Cancer

JF - BMC Cancer

SN - 1471-2407

ER -