The Egg Parasitoid Trissolcus basalis uses n-nonadecane, a Cuticular Hydrocarbon from its Stink Bug Host Nezara viridula, to Discriminate Between Female and Male Hosts

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Abstract

Contact kairomones from adult southern green stink bugs, Nezara viridula (L.) (Heteroptera: Pentatomidae) that elicit foraging behavior of the egg parasitoid Trissolcus basalis (Wollaston) were investigated in laboratory experiments. Chemical residues from tarsi and scutella of N. viridula induced foraging by gravid female T. basalis. Residues from body parts of female N. viridula elicited stronger responses than those from the corresponding body parts of males. Deproteinized tarsi still elicited searching responses from wasps, indicating that the kairomone was not proteinaceous. Hexane extracts of host cuticular lipids induced searching responses from T. basalis, with a strong preference for extracts from female hosts. Extracts consisted primarily of linear alkanes from nC(19) to nC(34), with quantitative and qualitative differences between the sexes. Extracts of female N. viridula contained more nC(23), nC(24), and nC(25) than the corresponding extracts from males, whereas nC(19) was detected only in extracts from males. Direct-contact solid phase microextraction (DC-SPME) of N. viridula cuticle and of residues left by adult bugs walking on a glass plate confirmed gender-specific differences in nC(19). Trissolcus basalis females responded weakly to a reconstructed blend of the straight-chain hydrocarbons, suggesting that minor components other than linear alkanes must be part of the kairomone. Addition of nC(19) to hexane extracts of female N. viridula significantly reduced the wasps' arrestment responses, similar to wasps' responses to hexane extracts of male hosts. Overall, our results suggest that a contact kairomone that elicits foraging by T. basalis females is present in the cuticular lipids of N. viridula, and that the presence or absence of nC(19) allows T. basalis females to distinguish between residues left by male or female hosts. The ecological significance of these results in the host location behavior of scelionid egg parasitoids is discussed.
Lingua originaleEnglish
pagine (da-a)1405-1420
Numero di pagine16
RivistaJournal of Chemical Ecology
Volume33
Stato di pubblicazionePublished - 2007

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Trissolcus basalis
egg parasitoid
Nezara viridula
Pentatomidae
Pheromones
Hydrocarbons
Ovum
hydrocarbons
Hexanes
hydrocarbon
kairomones
Alkanes
kairomone
extracts
Wasps
hexane
wasp
Lipids
foraging
alkanes

All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics
  • Biochemistry

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@article{fa780f34ce0a4cf5a1de86bc5f5f4589,
title = "The Egg Parasitoid Trissolcus basalis uses n-nonadecane, a Cuticular Hydrocarbon from its Stink Bug Host Nezara viridula, to Discriminate Between Female and Male Hosts",
abstract = "Contact kairomones from adult southern green stink bugs, Nezara viridula (L.) (Heteroptera: Pentatomidae) that elicit foraging behavior of the egg parasitoid Trissolcus basalis (Wollaston) were investigated in laboratory experiments. Chemical residues from tarsi and scutella of N. viridula induced foraging by gravid female T. basalis. Residues from body parts of female N. viridula elicited stronger responses than those from the corresponding body parts of males. Deproteinized tarsi still elicited searching responses from wasps, indicating that the kairomone was not proteinaceous. Hexane extracts of host cuticular lipids induced searching responses from T. basalis, with a strong preference for extracts from female hosts. Extracts consisted primarily of linear alkanes from nC(19) to nC(34), with quantitative and qualitative differences between the sexes. Extracts of female N. viridula contained more nC(23), nC(24), and nC(25) than the corresponding extracts from males, whereas nC(19) was detected only in extracts from males. Direct-contact solid phase microextraction (DC-SPME) of N. viridula cuticle and of residues left by adult bugs walking on a glass plate confirmed gender-specific differences in nC(19). Trissolcus basalis females responded weakly to a reconstructed blend of the straight-chain hydrocarbons, suggesting that minor components other than linear alkanes must be part of the kairomone. Addition of nC(19) to hexane extracts of female N. viridula significantly reduced the wasps' arrestment responses, similar to wasps' responses to hexane extracts of male hosts. Overall, our results suggest that a contact kairomone that elicits foraging by T. basalis females is present in the cuticular lipids of N. viridula, and that the presence or absence of nC(19) allows T. basalis females to distinguish between residues left by male or female hosts. The ecological significance of these results in the host location behavior of scelionid egg parasitoids is discussed.",
author = "Stefano Colazza and {De Pasquale}, Claudio and Ezio Peri and Millar, {Jocelyn G.}",
year = "2007",
language = "English",
volume = "33",
pages = "1405--1420",
journal = "Journal of Chemical Ecology",
issn = "0098-0331",
publisher = "Springer New York",

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TY - JOUR

T1 - The Egg Parasitoid Trissolcus basalis uses n-nonadecane, a Cuticular Hydrocarbon from its Stink Bug Host Nezara viridula, to Discriminate Between Female and Male Hosts

AU - Colazza, Stefano

AU - De Pasquale, Claudio

AU - Peri, Ezio

AU - Millar, Jocelyn G.

PY - 2007

Y1 - 2007

N2 - Contact kairomones from adult southern green stink bugs, Nezara viridula (L.) (Heteroptera: Pentatomidae) that elicit foraging behavior of the egg parasitoid Trissolcus basalis (Wollaston) were investigated in laboratory experiments. Chemical residues from tarsi and scutella of N. viridula induced foraging by gravid female T. basalis. Residues from body parts of female N. viridula elicited stronger responses than those from the corresponding body parts of males. Deproteinized tarsi still elicited searching responses from wasps, indicating that the kairomone was not proteinaceous. Hexane extracts of host cuticular lipids induced searching responses from T. basalis, with a strong preference for extracts from female hosts. Extracts consisted primarily of linear alkanes from nC(19) to nC(34), with quantitative and qualitative differences between the sexes. Extracts of female N. viridula contained more nC(23), nC(24), and nC(25) than the corresponding extracts from males, whereas nC(19) was detected only in extracts from males. Direct-contact solid phase microextraction (DC-SPME) of N. viridula cuticle and of residues left by adult bugs walking on a glass plate confirmed gender-specific differences in nC(19). Trissolcus basalis females responded weakly to a reconstructed blend of the straight-chain hydrocarbons, suggesting that minor components other than linear alkanes must be part of the kairomone. Addition of nC(19) to hexane extracts of female N. viridula significantly reduced the wasps' arrestment responses, similar to wasps' responses to hexane extracts of male hosts. Overall, our results suggest that a contact kairomone that elicits foraging by T. basalis females is present in the cuticular lipids of N. viridula, and that the presence or absence of nC(19) allows T. basalis females to distinguish between residues left by male or female hosts. The ecological significance of these results in the host location behavior of scelionid egg parasitoids is discussed.

AB - Contact kairomones from adult southern green stink bugs, Nezara viridula (L.) (Heteroptera: Pentatomidae) that elicit foraging behavior of the egg parasitoid Trissolcus basalis (Wollaston) were investigated in laboratory experiments. Chemical residues from tarsi and scutella of N. viridula induced foraging by gravid female T. basalis. Residues from body parts of female N. viridula elicited stronger responses than those from the corresponding body parts of males. Deproteinized tarsi still elicited searching responses from wasps, indicating that the kairomone was not proteinaceous. Hexane extracts of host cuticular lipids induced searching responses from T. basalis, with a strong preference for extracts from female hosts. Extracts consisted primarily of linear alkanes from nC(19) to nC(34), with quantitative and qualitative differences between the sexes. Extracts of female N. viridula contained more nC(23), nC(24), and nC(25) than the corresponding extracts from males, whereas nC(19) was detected only in extracts from males. Direct-contact solid phase microextraction (DC-SPME) of N. viridula cuticle and of residues left by adult bugs walking on a glass plate confirmed gender-specific differences in nC(19). Trissolcus basalis females responded weakly to a reconstructed blend of the straight-chain hydrocarbons, suggesting that minor components other than linear alkanes must be part of the kairomone. Addition of nC(19) to hexane extracts of female N. viridula significantly reduced the wasps' arrestment responses, similar to wasps' responses to hexane extracts of male hosts. Overall, our results suggest that a contact kairomone that elicits foraging by T. basalis females is present in the cuticular lipids of N. viridula, and that the presence or absence of nC(19) allows T. basalis females to distinguish between residues left by male or female hosts. The ecological significance of these results in the host location behavior of scelionid egg parasitoids is discussed.

UR - http://hdl.handle.net/10447/20879

UR - https://link.springer.com/article/10.1007/s10886-007-9389-8

M3 - Article

VL - 33

SP - 1405

EP - 1420

JO - Journal of Chemical Ecology

JF - Journal of Chemical Ecology

SN - 0098-0331

ER -