The transcription of two Ciona intestinalis galectin genes (CiLgals-a and CiLgalseb) is uparegulated by LPSin the pharynxis (hemocytes, vessel epithelium, endostilar zones) which is retained the main organ ofthe immunity. In this ascidian, for the first time we show, by immunohistochemistry and in situ hybridizationmethods, that these two immune-related genes are expressed in the gastric epithelium ofnaïve ascidians, whereas the galectins appear to be only contained in the intestine columnar epithelium.In addition, according to previous results on the pharynx, the genes are also expressed and galectinsproduced by hemocytes scattered in the connective tissue surrounding the gut. The genes expression andgalectin localization in several tissues, including the previous findings on the transcription upregulation,the constitutive expression of these genes by endostylar zones and by the gastric epithelium suggest apotential multifunctional role of these galectins. In this respect, it is of interest to define where theCiLgals are normally found as related to the tissue functions. Such an approach should be a starting pointfor further investigations.