The present work is dedicated to the study of the interactions of CdCl2 with the syntheticpolynucleotides polyAT and polyGC confined in the nanoscopic aqueous compartment of thewater-in-oil microemulsion CTAB/pentanol/hexane/water, with the goal to mimic in vitro thesituation met by the nucleic acids in vivo. In biological structures, in fact, very long strings ofnucleic acids are segregated into very small compartments having a radius exceedingly smallerthan the length of the encapsulated macromolecule. For comparison, the behaviour ofpolyGC was also studied in aqueous solutions of matched composition. The conformationaland thermal stabilities of both polynucleotides enclosed in the inner compartment of themicroemulsion are scarcely affected by the presence of CdCl2, whereas in solution immediateand large effects were observed also at room temperature. The lack of effects of CdCl2 on theproperties of the biopolymers entrapped in the aqueous core of the microemulsion has beenattributed to the peculiar characteristics of the medium (low dielectric constant, in particular)which cause a total repression of the CdCl2 dissociation that is not complete even in water.In fact, several of the numerous effects of CdCl2 observed on the conformational stability ofpolyGC in aqueous solutions have also been ascribed to the limited dissociation of thecadmium salt.
|Numero di pagine||12|
|Rivista||Physical Chemistry Chemical Physics|
|Stato di pubblicazione||Published - 2011|
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