The anti- apoptotic protein BAG3 is expressed in thyroid carcinomas and modulates apoptosis mediated by tumor necrosis factor- related apoptosis-inducing ligand (TRAIL).

Monica Zerilli, Giorgio Stassi, Matilde Todaro, Antonio Gentilella, Alessandra Rosati, Massimo Ammirante, Anna Basile, Mario Monaco, Emilia Vuttariello, Liberato Marzullo, Alessandra Tosco, Claudio Arra, Maria Antonietta Belisario, Gennaro Chiappetta, Rosa Pasquinelli, Luciano Pezzullo, Maria Pascale, Michela Festa, Maria Caterina Turco, Arturo LeoneAnna Basile

Risultato della ricerca: Article

87 Citazioni (Scopus)

Abstract

Context: We previously showed that BAG3 protein, a member of the BAG (Bcl-2-associated athanogene) co-chaperone family, modulates apoptosis in human leukemias. The expression of BAG3 in other tumor types has not been extensively investigated so far. Objective: The objective of this study was to analyze BAG3 expression in thyroid neoplastic cells and investigate its influence in cell apoptotic response to TNF-related apoptosis-inducing ligand (TRAIL). Design, Setting, and Patients: We investigated BAG3 expression in human thyroid carcinoma cell lines, including NPA, and the effect of BAG3-specific small interfering RNA on TRAIL-induced apoptosis in NPA cells. Subsequently, we analyzed BAG3 expression in 30 benign lesions and 56 carcinomas from patients of the Naples Tumor Institute Fondazione Senatore Pascale. Main Outcome Measures: The main outcome measures were: analysis of BAG3 protein in NPA cells by Western blot and immunocytochemistry; analysis of apoptosis in TRAIL-stimulated NPA cells by flow cytometry; and evaluation of BAG3 expression in specimens from thyroid lesions by immunohistochemistry. Results: BAG3 was expressed in human thyroid carcinoma cell lines; small interfering RNA-mediated downmodulation of its levels significantly (P < 0.0195) enhanced NPA cell apoptotic response to TRAIL. The protein was not detectable in 19 of 20 specimens of normal thyroid or goiters, whereas 54 of 56 analyzed carcinomas (15 follicular, 28 papillary, and 13 anaplastic) were clearly positive for BAG3 expression. Conclusions: BAG3 downmodulates the apoptotic response to TRAIL in human neoplastic thyroid cells. The protein is specifically expressed in thyroid carcinomas and not in normal thyroid tissue or goiter.
Lingua originaleEnglish
pagine (da-a)1159-1163
Numero di pagine5
RivistaTHE JOURNAL OF CLINICAL ENDOCRINOLOGY AND METABOLISM
Volume92
Stato di pubblicazionePublished - 2007

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Apoptosis Regulatory Proteins
Thyroid Neoplasms
Tumor Necrosis Factor-alpha
Apoptosis
Ligands
Thyroid Gland
Goiter
Small Interfering RNA
Tumors
Proteins
Immunohistochemistry
Cells
Outcome Assessment (Health Care)
TNF-Related Apoptosis-Inducing Ligand
Carcinoma
Cell Line
Flow cytometry
Neoplasms
Flow Cytometry
Leukemia

All Science Journal Classification (ASJC) codes

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Endocrinology
  • Clinical Biochemistry
  • Biochemistry, medical

Cita questo

The anti- apoptotic protein BAG3 is expressed in thyroid carcinomas and modulates apoptosis mediated by tumor necrosis factor- related apoptosis-inducing ligand (TRAIL). / Zerilli, Monica; Stassi, Giorgio; Todaro, Matilde; Gentilella, Antonio; Rosati, Alessandra; Ammirante, Massimo; Basile, Anna; Monaco, Mario; Vuttariello, Emilia; Marzullo, Liberato; Tosco, Alessandra; Arra, Claudio; Belisario, Maria Antonietta; Chiappetta, Gennaro; Pasquinelli, Rosa; Pezzullo, Luciano; Pascale, Maria; Festa, Michela; Turco, Maria Caterina; Leone, Arturo; Basile, Anna.

In: THE JOURNAL OF CLINICAL ENDOCRINOLOGY AND METABOLISM, Vol. 92, 2007, pag. 1159-1163.

Risultato della ricerca: Article

Zerilli, M, Stassi, G, Todaro, M, Gentilella, A, Rosati, A, Ammirante, M, Basile, A, Monaco, M, Vuttariello, E, Marzullo, L, Tosco, A, Arra, C, Belisario, MA, Chiappetta, G, Pasquinelli, R, Pezzullo, L, Pascale, M, Festa, M, Turco, MC, Leone, A & Basile, A 2007, 'The anti- apoptotic protein BAG3 is expressed in thyroid carcinomas and modulates apoptosis mediated by tumor necrosis factor- related apoptosis-inducing ligand (TRAIL).', THE JOURNAL OF CLINICAL ENDOCRINOLOGY AND METABOLISM, vol. 92, pagg. 1159-1163.
Zerilli, Monica ; Stassi, Giorgio ; Todaro, Matilde ; Gentilella, Antonio ; Rosati, Alessandra ; Ammirante, Massimo ; Basile, Anna ; Monaco, Mario ; Vuttariello, Emilia ; Marzullo, Liberato ; Tosco, Alessandra ; Arra, Claudio ; Belisario, Maria Antonietta ; Chiappetta, Gennaro ; Pasquinelli, Rosa ; Pezzullo, Luciano ; Pascale, Maria ; Festa, Michela ; Turco, Maria Caterina ; Leone, Arturo ; Basile, Anna. / The anti- apoptotic protein BAG3 is expressed in thyroid carcinomas and modulates apoptosis mediated by tumor necrosis factor- related apoptosis-inducing ligand (TRAIL). In: THE JOURNAL OF CLINICAL ENDOCRINOLOGY AND METABOLISM. 2007 ; Vol. 92. pagg. 1159-1163.
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title = "The anti- apoptotic protein BAG3 is expressed in thyroid carcinomas and modulates apoptosis mediated by tumor necrosis factor- related apoptosis-inducing ligand (TRAIL).",
abstract = "Context: We previously showed that BAG3 protein, a member of the BAG (Bcl-2-associated athanogene) co-chaperone family, modulates apoptosis in human leukemias. The expression of BAG3 in other tumor types has not been extensively investigated so far. Objective: The objective of this study was to analyze BAG3 expression in thyroid neoplastic cells and investigate its influence in cell apoptotic response to TNF-related apoptosis-inducing ligand (TRAIL). Design, Setting, and Patients: We investigated BAG3 expression in human thyroid carcinoma cell lines, including NPA, and the effect of BAG3-specific small interfering RNA on TRAIL-induced apoptosis in NPA cells. Subsequently, we analyzed BAG3 expression in 30 benign lesions and 56 carcinomas from patients of the Naples Tumor Institute Fondazione Senatore Pascale. Main Outcome Measures: The main outcome measures were: analysis of BAG3 protein in NPA cells by Western blot and immunocytochemistry; analysis of apoptosis in TRAIL-stimulated NPA cells by flow cytometry; and evaluation of BAG3 expression in specimens from thyroid lesions by immunohistochemistry. Results: BAG3 was expressed in human thyroid carcinoma cell lines; small interfering RNA-mediated downmodulation of its levels significantly (P < 0.0195) enhanced NPA cell apoptotic response to TRAIL. The protein was not detectable in 19 of 20 specimens of normal thyroid or goiters, whereas 54 of 56 analyzed carcinomas (15 follicular, 28 papillary, and 13 anaplastic) were clearly positive for BAG3 expression. Conclusions: BAG3 downmodulates the apoptotic response to TRAIL in human neoplastic thyroid cells. The protein is specifically expressed in thyroid carcinomas and not in normal thyroid tissue or goiter.",
author = "Monica Zerilli and Giorgio Stassi and Matilde Todaro and Antonio Gentilella and Alessandra Rosati and Massimo Ammirante and Anna Basile and Mario Monaco and Emilia Vuttariello and Liberato Marzullo and Alessandra Tosco and Claudio Arra and Belisario, {Maria Antonietta} and Gennaro Chiappetta and Rosa Pasquinelli and Luciano Pezzullo and Maria Pascale and Michela Festa and Turco, {Maria Caterina} and Arturo Leone and Anna Basile",
year = "2007",
language = "English",
volume = "92",
pages = "1159--1163",
journal = "Journal of Clinical Endocrinology and Metabolism",
issn = "0021-972X",
publisher = "Oxford University Press",

}

TY - JOUR

T1 - The anti- apoptotic protein BAG3 is expressed in thyroid carcinomas and modulates apoptosis mediated by tumor necrosis factor- related apoptosis-inducing ligand (TRAIL).

AU - Zerilli, Monica

AU - Stassi, Giorgio

AU - Todaro, Matilde

AU - Gentilella, Antonio

AU - Rosati, Alessandra

AU - Ammirante, Massimo

AU - Basile, Anna

AU - Monaco, Mario

AU - Vuttariello, Emilia

AU - Marzullo, Liberato

AU - Tosco, Alessandra

AU - Arra, Claudio

AU - Belisario, Maria Antonietta

AU - Chiappetta, Gennaro

AU - Pasquinelli, Rosa

AU - Pezzullo, Luciano

AU - Pascale, Maria

AU - Festa, Michela

AU - Turco, Maria Caterina

AU - Leone, Arturo

AU - Basile, Anna

PY - 2007

Y1 - 2007

N2 - Context: We previously showed that BAG3 protein, a member of the BAG (Bcl-2-associated athanogene) co-chaperone family, modulates apoptosis in human leukemias. The expression of BAG3 in other tumor types has not been extensively investigated so far. Objective: The objective of this study was to analyze BAG3 expression in thyroid neoplastic cells and investigate its influence in cell apoptotic response to TNF-related apoptosis-inducing ligand (TRAIL). Design, Setting, and Patients: We investigated BAG3 expression in human thyroid carcinoma cell lines, including NPA, and the effect of BAG3-specific small interfering RNA on TRAIL-induced apoptosis in NPA cells. Subsequently, we analyzed BAG3 expression in 30 benign lesions and 56 carcinomas from patients of the Naples Tumor Institute Fondazione Senatore Pascale. Main Outcome Measures: The main outcome measures were: analysis of BAG3 protein in NPA cells by Western blot and immunocytochemistry; analysis of apoptosis in TRAIL-stimulated NPA cells by flow cytometry; and evaluation of BAG3 expression in specimens from thyroid lesions by immunohistochemistry. Results: BAG3 was expressed in human thyroid carcinoma cell lines; small interfering RNA-mediated downmodulation of its levels significantly (P < 0.0195) enhanced NPA cell apoptotic response to TRAIL. The protein was not detectable in 19 of 20 specimens of normal thyroid or goiters, whereas 54 of 56 analyzed carcinomas (15 follicular, 28 papillary, and 13 anaplastic) were clearly positive for BAG3 expression. Conclusions: BAG3 downmodulates the apoptotic response to TRAIL in human neoplastic thyroid cells. The protein is specifically expressed in thyroid carcinomas and not in normal thyroid tissue or goiter.

AB - Context: We previously showed that BAG3 protein, a member of the BAG (Bcl-2-associated athanogene) co-chaperone family, modulates apoptosis in human leukemias. The expression of BAG3 in other tumor types has not been extensively investigated so far. Objective: The objective of this study was to analyze BAG3 expression in thyroid neoplastic cells and investigate its influence in cell apoptotic response to TNF-related apoptosis-inducing ligand (TRAIL). Design, Setting, and Patients: We investigated BAG3 expression in human thyroid carcinoma cell lines, including NPA, and the effect of BAG3-specific small interfering RNA on TRAIL-induced apoptosis in NPA cells. Subsequently, we analyzed BAG3 expression in 30 benign lesions and 56 carcinomas from patients of the Naples Tumor Institute Fondazione Senatore Pascale. Main Outcome Measures: The main outcome measures were: analysis of BAG3 protein in NPA cells by Western blot and immunocytochemistry; analysis of apoptosis in TRAIL-stimulated NPA cells by flow cytometry; and evaluation of BAG3 expression in specimens from thyroid lesions by immunohistochemistry. Results: BAG3 was expressed in human thyroid carcinoma cell lines; small interfering RNA-mediated downmodulation of its levels significantly (P < 0.0195) enhanced NPA cell apoptotic response to TRAIL. The protein was not detectable in 19 of 20 specimens of normal thyroid or goiters, whereas 54 of 56 analyzed carcinomas (15 follicular, 28 papillary, and 13 anaplastic) were clearly positive for BAG3 expression. Conclusions: BAG3 downmodulates the apoptotic response to TRAIL in human neoplastic thyroid cells. The protein is specifically expressed in thyroid carcinomas and not in normal thyroid tissue or goiter.

UR - http://hdl.handle.net/10447/2515

M3 - Article

VL - 92

SP - 1159

EP - 1163

JO - Journal of Clinical Endocrinology and Metabolism

JF - Journal of Clinical Endocrinology and Metabolism

SN - 0021-972X

ER -