Background: There is an increased number of activated Tl lymphocytes in the bronchial mucosa of stable COPD patients but are absent studies on Th17 cells and their effector cytokines.Objectives: To investigate the expression of retinoic orphan receptor(ROR)C2,interleukin(IL)-17A,IL-17F,IL-21,IL-22 and IL-23 in bronchial biopsies frompatients with stable COPD of different severity(stage 2 to 4)compared withage-matched control subject(smokers with normal lung function and lifelongnon-smokers).Methods: The expression of RORC2,IL-17A,IL-17F,IL-21,IL-22 and IL-23was measured in the bronchial mucosa using immunohistochemistry and/or realtime quantitative polymerase chain reaction.Results: The number of IL-22+ and IL23+ immunoreactive cells is increasedin the bronchial epithelium of stable COPD compared with control groups. Inaddition, the number of IL-17A+ and IL22+ immunoreactive cells is increased inthe bronchial submucosa of stable COPD compared with control non-smokers. Inall smokers with and without disease and in patients with COPD alone the numberof IL22+ cells is correlated significantly with the number of both CD4+ and CD8+ cells in the bronchial mucosa.The expression of the RORC2 mRNA in the bronchial mucosa was not significantly different between smokers with normal lung function and COPD.Conclusion: Thei ncreased expression of the Th17-related cytokines IL-17A,IL-22 and IL-23 maybe involved in the recruitment and activation of lymphocytes populating the bronchial mucosa and in the host defence developing in patients with stable COPD.
|Numero di pagine||1|
|Stato di pubblicazione||Published - 2009|