TGF-β Signaling Pathways in Different Compartments of the Lower Airways of Patients With Stable COPD

Claudia Sangiorgi, Francesco Cappello, Yike Guo, Paolo Ruggeri, Kian Fan Chung, Peter J. Barnes, Paola Brun, Giuseppe Girbino, Marco Contoli, Claudia Sangiorgi, Pio Maniscalco, Stelios Pavlides, Antonino Di Stefano, Isabella Gnemmi, Bruno Balbi, Gaetano Caramori, Fabio L.M. Ricciardolo, Alberto Papi, Ian M. Adcock, Paolo Casolari

Risultato della ricerca: Article

8 Citazioni (Scopus)

Abstract

Background: The expression and localization of transforming growth factor-β (TGF-β) pathway proteins in different compartments of the lower airways of patients with stable COPD is unclear. We aimed to determine TGF-β pathway protein expression in patients with stable COPD. Methods: The expression and localization of TGF-β pathway components was measured in the bronchial mucosa and peripheral lungs of patients with stable COPD (n = 44), control smokers with normal lung function (n = 24), and control nonsmoking subjects (n = 11) using immunohistochemical analysis. Results: TGF-β1, TGF-β3, and connective tissue growth factor expression were significantly decreased in the bronchiolar epithelium, with TGF-β1 also decreased in alveolar macrophages, in patients with stable COPD compared with control smokers with normal lung function. TGF-β3 expression was increased in the bronchial lamina propria of both control smokers with normal lung function and smokers with mild/moderate stable COPD compared with control nonsmokers and correlated significantly with pack-years of smoking. However, TGF-β3+cells decreased in patients with severe/very severe COPD compared with control smokers. Latent TGF-β binding protein 1 expression was increased in the bronchial lamina propria in subjects with stable COPD of all severities compared with control smokers with normal lung function. Bone morphogenetic protein and activin membrane-bound inhibitor expression (BAMBI) in the bronchial mucosa was significantly increased in patients with stable COPD of all severities compared with control subjects. No other significant differences were observed between groups for all the other molecules studied in the bronchial mucosa and peripheral lung. Conclusions: Expression of TGF-βs and their regulatory proteins is distinct within different lower airway compartments in stable COPD. Selective reduction in TGF-β1 and enhanced BAMBI expression may be associated with the increase in autoimmunity in COPD.
Lingua originaleEnglish
pagine (da-a)851-862
Numero di pagine12
RivistaDefault journal
Volume153
Stato di pubblicazionePublished - 2018

All Science Journal Classification (ASJC) codes

  • Pulmonary and Respiratory Medicine
  • Critical Care and Intensive Care Medicine
  • Cardiology and Cardiovascular Medicine

Cita questo

Sangiorgi, C., Cappello, F., Guo, Y., Ruggeri, P., Chung, K. F., Barnes, P. J., ... Casolari, P. (2018). TGF-β Signaling Pathways in Different Compartments of the Lower Airways of Patients With Stable COPD. Default journal, 153, 851-862.

TGF-β Signaling Pathways in Different Compartments of the Lower Airways of Patients With Stable COPD. / Sangiorgi, Claudia; Cappello, Francesco; Guo, Yike; Ruggeri, Paolo; Chung, Kian Fan; Barnes, Peter J.; Brun, Paola; Girbino, Giuseppe; Contoli, Marco; Sangiorgi, Claudia; Maniscalco, Pio; Pavlides, Stelios; Di Stefano, Antonino; Gnemmi, Isabella; Balbi, Bruno; Caramori, Gaetano; Ricciardolo, Fabio L.M.; Papi, Alberto; Adcock, Ian M.; Casolari, Paolo.

In: Default journal, Vol. 153, 2018, pag. 851-862.

Risultato della ricerca: Article

Sangiorgi, C, Cappello, F, Guo, Y, Ruggeri, P, Chung, KF, Barnes, PJ, Brun, P, Girbino, G, Contoli, M, Sangiorgi, C, Maniscalco, P, Pavlides, S, Di Stefano, A, Gnemmi, I, Balbi, B, Caramori, G, Ricciardolo, FLM, Papi, A, Adcock, IM & Casolari, P 2018, 'TGF-β Signaling Pathways in Different Compartments of the Lower Airways of Patients With Stable COPD', Default journal, vol. 153, pagg. 851-862.
Sangiorgi, Claudia ; Cappello, Francesco ; Guo, Yike ; Ruggeri, Paolo ; Chung, Kian Fan ; Barnes, Peter J. ; Brun, Paola ; Girbino, Giuseppe ; Contoli, Marco ; Sangiorgi, Claudia ; Maniscalco, Pio ; Pavlides, Stelios ; Di Stefano, Antonino ; Gnemmi, Isabella ; Balbi, Bruno ; Caramori, Gaetano ; Ricciardolo, Fabio L.M. ; Papi, Alberto ; Adcock, Ian M. ; Casolari, Paolo. / TGF-β Signaling Pathways in Different Compartments of the Lower Airways of Patients With Stable COPD. In: Default journal. 2018 ; Vol. 153. pagg. 851-862.
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title = "TGF-β Signaling Pathways in Different Compartments of the Lower Airways of Patients With Stable COPD",
abstract = "Background: The expression and localization of transforming growth factor-β (TGF-β) pathway proteins in different compartments of the lower airways of patients with stable COPD is unclear. We aimed to determine TGF-β pathway protein expression in patients with stable COPD. Methods: The expression and localization of TGF-β pathway components was measured in the bronchial mucosa and peripheral lungs of patients with stable COPD (n = 44), control smokers with normal lung function (n = 24), and control nonsmoking subjects (n = 11) using immunohistochemical analysis. Results: TGF-β1, TGF-β3, and connective tissue growth factor expression were significantly decreased in the bronchiolar epithelium, with TGF-β1 also decreased in alveolar macrophages, in patients with stable COPD compared with control smokers with normal lung function. TGF-β3 expression was increased in the bronchial lamina propria of both control smokers with normal lung function and smokers with mild/moderate stable COPD compared with control nonsmokers and correlated significantly with pack-years of smoking. However, TGF-β3+cells decreased in patients with severe/very severe COPD compared with control smokers. Latent TGF-β binding protein 1 expression was increased in the bronchial lamina propria in subjects with stable COPD of all severities compared with control smokers with normal lung function. Bone morphogenetic protein and activin membrane-bound inhibitor expression (BAMBI) in the bronchial mucosa was significantly increased in patients with stable COPD of all severities compared with control subjects. No other significant differences were observed between groups for all the other molecules studied in the bronchial mucosa and peripheral lung. Conclusions: Expression of TGF-βs and their regulatory proteins is distinct within different lower airway compartments in stable COPD. Selective reduction in TGF-β1 and enhanced BAMBI expression may be associated with the increase in autoimmunity in COPD.",
keywords = "BAMBI, CTGF, Cardiology and Cardiovascular Medicine, Critical Care and Intensive Care Medicine, Pulmonary and Respiratory Medicine, SMAD, TGF-β, airway inflammation, autoimmunity",
author = "Claudia Sangiorgi and Francesco Cappello and Yike Guo and Paolo Ruggeri and Chung, {Kian Fan} and Barnes, {Peter J.} and Paola Brun and Giuseppe Girbino and Marco Contoli and Claudia Sangiorgi and Pio Maniscalco and Stelios Pavlides and {Di Stefano}, Antonino and Isabella Gnemmi and Bruno Balbi and Gaetano Caramori and Ricciardolo, {Fabio L.M.} and Alberto Papi and Adcock, {Ian M.} and Paolo Casolari",
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TY - JOUR

T1 - TGF-β Signaling Pathways in Different Compartments of the Lower Airways of Patients With Stable COPD

AU - Sangiorgi, Claudia

AU - Cappello, Francesco

AU - Guo, Yike

AU - Ruggeri, Paolo

AU - Chung, Kian Fan

AU - Barnes, Peter J.

AU - Brun, Paola

AU - Girbino, Giuseppe

AU - Contoli, Marco

AU - Sangiorgi, Claudia

AU - Maniscalco, Pio

AU - Pavlides, Stelios

AU - Di Stefano, Antonino

AU - Gnemmi, Isabella

AU - Balbi, Bruno

AU - Caramori, Gaetano

AU - Ricciardolo, Fabio L.M.

AU - Papi, Alberto

AU - Adcock, Ian M.

AU - Casolari, Paolo

PY - 2018

Y1 - 2018

N2 - Background: The expression and localization of transforming growth factor-β (TGF-β) pathway proteins in different compartments of the lower airways of patients with stable COPD is unclear. We aimed to determine TGF-β pathway protein expression in patients with stable COPD. Methods: The expression and localization of TGF-β pathway components was measured in the bronchial mucosa and peripheral lungs of patients with stable COPD (n = 44), control smokers with normal lung function (n = 24), and control nonsmoking subjects (n = 11) using immunohistochemical analysis. Results: TGF-β1, TGF-β3, and connective tissue growth factor expression were significantly decreased in the bronchiolar epithelium, with TGF-β1 also decreased in alveolar macrophages, in patients with stable COPD compared with control smokers with normal lung function. TGF-β3 expression was increased in the bronchial lamina propria of both control smokers with normal lung function and smokers with mild/moderate stable COPD compared with control nonsmokers and correlated significantly with pack-years of smoking. However, TGF-β3+cells decreased in patients with severe/very severe COPD compared with control smokers. Latent TGF-β binding protein 1 expression was increased in the bronchial lamina propria in subjects with stable COPD of all severities compared with control smokers with normal lung function. Bone morphogenetic protein and activin membrane-bound inhibitor expression (BAMBI) in the bronchial mucosa was significantly increased in patients with stable COPD of all severities compared with control subjects. No other significant differences were observed between groups for all the other molecules studied in the bronchial mucosa and peripheral lung. Conclusions: Expression of TGF-βs and their regulatory proteins is distinct within different lower airway compartments in stable COPD. Selective reduction in TGF-β1 and enhanced BAMBI expression may be associated with the increase in autoimmunity in COPD.

AB - Background: The expression and localization of transforming growth factor-β (TGF-β) pathway proteins in different compartments of the lower airways of patients with stable COPD is unclear. We aimed to determine TGF-β pathway protein expression in patients with stable COPD. Methods: The expression and localization of TGF-β pathway components was measured in the bronchial mucosa and peripheral lungs of patients with stable COPD (n = 44), control smokers with normal lung function (n = 24), and control nonsmoking subjects (n = 11) using immunohistochemical analysis. Results: TGF-β1, TGF-β3, and connective tissue growth factor expression were significantly decreased in the bronchiolar epithelium, with TGF-β1 also decreased in alveolar macrophages, in patients with stable COPD compared with control smokers with normal lung function. TGF-β3 expression was increased in the bronchial lamina propria of both control smokers with normal lung function and smokers with mild/moderate stable COPD compared with control nonsmokers and correlated significantly with pack-years of smoking. However, TGF-β3+cells decreased in patients with severe/very severe COPD compared with control smokers. Latent TGF-β binding protein 1 expression was increased in the bronchial lamina propria in subjects with stable COPD of all severities compared with control smokers with normal lung function. Bone morphogenetic protein and activin membrane-bound inhibitor expression (BAMBI) in the bronchial mucosa was significantly increased in patients with stable COPD of all severities compared with control subjects. No other significant differences were observed between groups for all the other molecules studied in the bronchial mucosa and peripheral lung. Conclusions: Expression of TGF-βs and their regulatory proteins is distinct within different lower airway compartments in stable COPD. Selective reduction in TGF-β1 and enhanced BAMBI expression may be associated with the increase in autoimmunity in COPD.

KW - BAMBI

KW - CTGF

KW - Cardiology and Cardiovascular Medicine

KW - Critical Care and Intensive Care Medicine

KW - Pulmonary and Respiratory Medicine

KW - SMAD

KW - TGF-β

KW - airway inflammation

KW - autoimmunity

UR - http://hdl.handle.net/10447/295433

UR - http://www.chestjournal.org/

M3 - Article

VL - 153

SP - 851

EP - 862

JO - Default journal

JF - Default journal

ER -