TEM morphological characterization of a one-step self-etching system applied clinically to human caries-affected dentin and deep sound dentin

Giuseppe Gallina, Antonella Bortone, Giorgio Castelli, Salvatore Marceddu, Egle Milia, Franklin Garcia-Godoy, Roberto Pinna

Risultato della ricerca: Article

8 Citazioni (Scopus)


Purpose: To examine morphologically the hybrid layer of one-step self-etching adhesive, Clearfil S3 Bond (S3bond), in caries-affected dentin (CAD) and deep sound dentin (DSD) cavities performed clinically. For a comparative group, the two-step self etching adhesive Clearfil Protect Bond (Pbond) was used in a similar clinical situation. Methods: This study was carried out on carious and sound teeth clinically selected for extraction. In carious teeth, CAD was obtained by way of subjective criteria by removing infected tissue to form a cavity bottom. DSD was obtained at a depth of 4 mm in the dentin cavity of sound teeth. S3bond and Pbond were applied in the CAD and DSD cavities as indicated by the manufacturer, followed by a composite restoration. Teeth were extracted about 20 minutes after the bonding procedure, and processed for TEM analysis. Results: Expression of S 3bond in CAD was morphologically highly variable. When affected tubules were occluded by intratubular mineralized deposits, the interface displayed a dense poly-HEMA hydrogel as water sorption by the hydrophilic S 3bond toward the porous affected collagen. Conversely, when tubules appeared empty, voids of various sizes were formed by tubular fluid shift. In DSD, S bond clearly exhibited voids and water channels as signs of the high permeability of the sub-surface. Although porosities were somewhat retained, Pbond expression showed a hermetic character which was independent of the dentin substrate. S3bond bonding to dentin was compromised as shown by signs of water movement within the resin/dentin bond outlining a weak capacity to produce an impermeable hybridiztion.
Lingua originaleEnglish
pagine (da-a)321-326
Numero di pagine6
RivistaAmerican Journal of Dentistry
Stato di pubblicazionePublished - 2012


All Science Journal Classification (ASJC) codes

  • Dentistry(all)

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