Telomerase reverse transcriptase germline mutations and hepatocellular carcinoma in patients with nonalcoholic fatty liver disease

Stefania Grimaudo, Antonio Craxi, Salvatore Petta, Quentin M. Anstee, Benedetta Donati, Alessandro Pietrelli, Serena Pelusi, Rosellina Margherita Mancina, Chiara Rosso, Piero Pingitore, Laura De Luca, Antonio Grieco, Fabio Colli, Andrea Caddeo, Guido Baselli, Silvia Maier, Lucy Walker, Giorgio Soardo, Stefano Romeo, Laura De LucaEster Vanni, Paola Dongiovanni, Elisabetta Bugianesi, Anna Ludovica Fracanzani, Luca Valenti, Helen L. Reeves, Luca Miele, Silvia Fargion, Renato Romagnoli, Ann Daly

Risultato della ricerca: Article

16 Citazioni (Scopus)

Abstract

In an increasing proportion of cases, hepatocellular carcinoma (HCC) develops in patients with nonalcoholic fatty liver disease (NAFLD). Mutations in telomerase reverse transcriptase (hTERT) are associated with familial liver diseases. The aim of this study was to examine telomere length and germline hTERT mutations as associated with NAFLD-HCC. In 40 patients with NAFLD-HCC, 45 with NAFLD-cirrhosis and 64 healthy controls, peripheral blood telomere length was evaluated by qRT-PCR and hTERT coding regions and intron-exon boundaries sequenced. We further analyzed 78 patients affected by primary liver cancer (NAFLD-PLC, 76 with HCC). Enrichment of rare coding mutations (allelic frequency <0.001) was evaluated by Burden test. Functional consequences were estimated in silico and by over-expressing protein variants in HEK-293 cells. We found that telomere length was reduced in individuals with NAFLD-HCC versus those with cirrhosis (P = 0.048) and healthy controls (P = 0.0006), independently of age and sex. We detected an enrichment of hTERT mutations in NAFLD-HCC, that was confirmed when we further considered a larger cohort of NAFLD-PLC, and was more marked in female patients (P = 0.03). No mutations were found in cirrhosis and local controls, and only one in 503 healthy Europeans from the 1000 Genomes Project (allelic frequency = 0.025 vs. <0.001; P = 0.0005). Mutations with predicted functional impact, including the frameshift Glu113Argfs*79 and missense Glu668Asp, cosegregated with liver disease in two families. Three patients carried missense mutations (Ala67Val in homozygosity, Pro193Leu and His296Pro in heterozygosity) in the N-terminal template-binding. domain (P = 0.037 for specific enrichment). Besides Glu668Asp, the Ala67Val variant resulted in reduced intracellular protein levels. In conclusion, we detected an association between shorter telomeres in peripheral blood and rare germline hTERT mutations and NAFLD-HCC.
Lingua originaleEnglish
pagine (da-a)1930-1940
Numero di pagine11
RivistaCancer Medicine
Volume6
Stato di pubblicazionePublished - 2017

All Science Journal Classification (ASJC) codes

  • Oncology
  • Radiology Nuclear Medicine and imaging
  • Cancer Research

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    Grimaudo, S., Craxi, A., Petta, S., Anstee, Q. M., Donati, B., Pietrelli, A., Pelusi, S., Mancina, R. M., Rosso, C., Pingitore, P., De Luca, L., Grieco, A., Colli, F., Caddeo, A., Baselli, G., Maier, S., Walker, L., Soardo, G., Romeo, S., ... Daly, A. (2017). Telomerase reverse transcriptase germline mutations and hepatocellular carcinoma in patients with nonalcoholic fatty liver disease. Cancer Medicine, 6, 1930-1940.