Techniques to Analyze sRNA Protein Cofactor Self-Assembly In Vitro

Valeria Militello, Sylvain Trépout, Thomas Bizien, Antoine Malabirade, David Partouche, Sergio Marco, Frank Wien, Christophe Sandt, Véronique Arluison, Marisela Velez

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3 Citazioni (Scopus)

Abstract

Post-transcriptional control of gene expression by small regulatory noncoding RNA (sRNA) needs protein accomplices to occur. Past research mainly focused on the RNA chaperone Hfq as cofactor. Nevertheless, recent studies indicated that other proteins might be involved in sRNA-based regulations. As some of these proteins have been shown to self-assemble, we describe in this chapter protocols to analyze the nano-assemblies formed. Precisely, we focus our analysis on Escherichia coli Hfq as a model, but the protocols presented here can be applied to analyze any polymer of proteins. This chapter thus provides a guideline to develop commonly used approaches to detect prokaryotic protein self-assembly, with a special focus on the detection of amyloidogenic polymers.
Lingua originaleEnglish
Titolo della pubblicazione ospiteMethods in Molecular Biology - Bacterial Regulatory RNA
Pagine321-340
Numero di pagine20
Stato di pubblicazionePublished - 2018

All Science Journal Classification (ASJC) codes

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  • ???subjectarea.asjc.1300.1311???

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