Technical Advance:Soluble OX40 molecule mimics regulatory T cell modulatory activity on Fc{varepsilon}RI-dependent mast cell degranulation.

Claudio Tripodo, Ribatti, Blengio, Audrito, Bertolotto, Ferretti, Gaidano, Silvia Deaglio, Simona Zupo, Corcione, Ottonello, Fabio Malavasi, Pistoia, Davide Rossi, Matis

Risultato della ricerca: Articlepeer review

37 Citazioni (Scopus)

Abstract

Tregs play a central role in modulating FcεRI-dependent MC effector functions in the course of the allergic response. Cellular interaction depends on the constitutive expression of OX40 on Tregs and the OX40L counterpart on MCs. Study of OX40L signaling on MCs is hampered by the need of a highly purified molecule, which triggers OX40L specifically. We now report that sOX40 mimics the physiological activity of Treg interaction by binding to activated MCs. When treated with sOX40, activated MCs showed decreased degranulation and Ca++ influx, whereas PLC-γ2 phosphorylation remained unaffected. Once injected into experimental animals, sOX40 not only located within the endothelium but also in parenchyma, where it could be found in close proximity and apparently bound to MCs. This soluble molecule triggers MC-OX40L without the requirement of Tregs, thus allowing study of OX40L signaling pathways in MCs and in other OX40L-expressing cell populations. Importantly, as sOX40 inhibits MC degranulation, it may provide an in vivo therapeutic tool in allergic disease.
Lingua originaleEnglish
pagine (da-a)831-838
Numero di pagine8
RivistaJournal of Leukocyte Biology
Volume90
Stato di pubblicazionePublished - 2011

All Science Journal Classification (ASJC) codes

  • Hematology
  • Oncology
  • Cancer Research

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