T-Cell Subsets (TCM, TEM, TEMRA) and Poly-Functional Immune Response in Patients with Human Immunodeficiency Virus (HIV) Infection and Different T-CD4 Cell Response

Serena Meraviglia, Paola Di Carlo, Francesco Dieli, Diego Pampinella, Giulia Marchetti, Consolato Sergi, Giulia Marchetti, Concetta Sergi, Diego Pampinella, Giuliana Guadagnino, Valentina Orlando

Risultato della ricerca: Articlepeer review

Abstract

OBJECTIVE: Dynamic changes of cytotoxic T cell responses against Human Immunodeficiency Virus 1 (HIV-1) infection have been the subject of an innovative investigation using antiretroviral therapy (ART). Currently, human CD8 naïve central memory (TCM), effector memory (TEM), and effector memory cells re-expressing CD45RA (TEMRA) T-cells have been thoroughly studied with ART. CD45RA is a marker usually found on naïve T-cells. MATERIALS AND METHODS: We performed a longitudinal study of mono-/polyfunctional T-cells in the peripheral blood while targeting three functionally distinct cell populations of CD4+ and CD8+ T-cells (single IL2 and IFN-γ, dual IL2/IFN-γ) in 50 HIV-1 patients. These patients consisted of 5 controllers, 15 non-controllers, 20 ART responders, and 10 highly active antiretroviral therapy (HAART) non-responders. RESULTS: We found that (1) non-controllers had the highest rate of IFN-γ-expressing CD4 or CD8, but the lowest rate of IL2-producing CD4 or CD8. (2) The control of HIV-1 infection was associated with polyfunctional Gag-specific T cell responses in controllers and responders. (3) Non-responders had high serum levels of IL2 and IFN-γ. There was a high percentage of CD4+ T cell response cells within the less differentiated phenotype in controllers. CD8+ T cell showed a high rate of TEM and TEMRA in responders. CONCLUSION: High levels of pro-inflammatory cytokines are typical in non-responders, exhausted T-cells may be associated with HIV-1 progression.
Lingua originaleEnglish
pagine (da-a)519-528
Numero di pagine10
RivistaAnnals of Clinical and Laboratory Science
Volume49
Stato di pubblicazionePublished - 2019

All Science Journal Classification (ASJC) codes

  • ???subjectarea.asjc.2700.2700???
  • ???subjectarea.asjc.2400.2404???
  • ???subjectarea.asjc.2700.2723???
  • ???subjectarea.asjc.2700.2734???
  • ???subjectarea.asjc.2400.2403???
  • ???subjectarea.asjc.1300.1312???
  • ???subjectarea.asjc.2700.2720???
  • ???subjectarea.asjc.1300.1308???
  • ???subjectarea.asjc.3600.3607???

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