Synthesis, characterization and antimicrobial activity of polyaminocyclodextrin-capped Ag Nanoparticles

Alessandro, M.

Risultato della ricerca: Paper

Abstract

Biocompatible Ag nanocomposites were prepared by photoreduction of ammoniacal silver acetate in the presence of a polyaminocyclodextrin, namely the poly-{6-[3-(2-(3-aminopropylamino)-ethylamino)-propylamino]}-(6-deoxy)-b-CD (amCD, figure 1). The obtained Ag-amCD systems, which possess an oniontype structure [1] with a metal core surrounded by several layers of the capping agent, were characterized by means of various complementary techniques. In particular, FT-IR spectroscopy confirmed the presence of the amCD scaffold in the composite, and evidenced a partial oxidative degradation of the polyamine branches,due to the fact that these groups function as sacrificial reducing agents in the photoinduced process of formation of the Ag metal core. TEM and SAED micrographs evidenced that the Ag cores possess a relatively low polydispersity and a significantly crystalline character.Then, in consideration of the well-known antimicrobial activityof nanosized silver, our Ag-amCD systems were assayed for antibacterial activity, quantified as the minimal concentration inhibiting at least the 90% of bacterial growth (MIC90), using Escherichia coli and Kocuria rhizophila as Gram-negative and Gram-positive tester strains. This analysis revealed 5 and 1 μg/ml as MIC90 values against E. coli and K. rhizophila; respectively. In addition, thanks to their peculiar features, the systems function as potential supramolecular drug carriers, effectively able to bind the b-lactam antibiotic Ampicillin (amp, figure 2) as demonstrated by polarimetric measurements. Antimicrobial assays reveals a five-fold improved activity of Ag-amCD-amp probablydue to synergistic action of Agnanoparticles and amp. This studyprovides insights on the attractive possibility to use an environmentally-friendly methodology to produce bioactive supramolecular systemstobeemployedaspowerful andtunableantimicrobial agents. [1] M. Russo, F. Armetta, S. Riela, D. Chillura Martino, P. Lo Meo, R. Noto J. Mol. Cat. A 408 (2015) 250-261.
Lingua originaleEnglish
Stato di pubblicazionePublished - 2015

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Escherichia coli
Metals
Nanoparticles
Lactams
Drug Carriers
Reducing Agents
Polyamines
Polydispersity
Ampicillin
Silver
Scaffolds
Infrared spectroscopy
Assays
Nanocomposites
Anti-Bacterial Agents
Crystalline materials
Transmission electron microscopy
Degradation
Composite materials
silver acetate

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title = "Synthesis, characterization and antimicrobial activity of polyaminocyclodextrin-capped Ag Nanoparticles",
abstract = "Biocompatible Ag nanocomposites were prepared by photoreduction of ammoniacal silver acetate in the presence of a polyaminocyclodextrin, namely the poly-{6-[3-(2-(3-aminopropylamino)-ethylamino)-propylamino]}-(6-deoxy)-b-CD (amCD, figure 1). The obtained Ag-amCD systems, which possess an oniontype structure [1] with a metal core surrounded by several layers of the capping agent, were characterized by means of various complementary techniques. In particular, FT-IR spectroscopy confirmed the presence of the amCD scaffold in the composite, and evidenced a partial oxidative degradation of the polyamine branches,due to the fact that these groups function as sacrificial reducing agents in the photoinduced process of formation of the Ag metal core. TEM and SAED micrographs evidenced that the Ag cores possess a relatively low polydispersity and a significantly crystalline character.Then, in consideration of the well-known antimicrobial activityof nanosized silver, our Ag-amCD systems were assayed for antibacterial activity, quantified as the minimal concentration inhibiting at least the 90{\%} of bacterial growth (MIC90), using Escherichia coli and Kocuria rhizophila as Gram-negative and Gram-positive tester strains. This analysis revealed 5 and 1 μg/ml as MIC90 values against E. coli and K. rhizophila; respectively. In addition, thanks to their peculiar features, the systems function as potential supramolecular drug carriers, effectively able to bind the b-lactam antibiotic Ampicillin (amp, figure 2) as demonstrated by polarimetric measurements. Antimicrobial assays reveals a five-fold improved activity of Ag-amCD-amp probablydue to synergistic action of Agnanoparticles and amp. This studyprovides insights on the attractive possibility to use an environmentally-friendly methodology to produce bioactive supramolecular systemstobeemployedaspowerful andtunableantimicrobial agents. [1] M. Russo, F. Armetta, S. Riela, D. Chillura Martino, P. Lo Meo, R. Noto J. Mol. Cat. A 408 (2015) 250-261.",
author = "{Alessandro, M.} and Renato Noto and Eugenio Caponetti and {Lo Meo}, {Paolo Maria Giuseppe} and {Chillura Martino}, {Delia Francesca} and Giuseppe Gallo and Alberto Sutera and Marco Russo",
year = "2015",
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TY - CONF

T1 - Synthesis, characterization and antimicrobial activity of polyaminocyclodextrin-capped Ag Nanoparticles

AU - Alessandro, M.

AU - Noto, Renato

AU - Caponetti, Eugenio

AU - Lo Meo, Paolo Maria Giuseppe

AU - Chillura Martino, Delia Francesca

AU - Gallo, Giuseppe

AU - Sutera, Alberto

AU - Russo, Marco

PY - 2015

Y1 - 2015

N2 - Biocompatible Ag nanocomposites were prepared by photoreduction of ammoniacal silver acetate in the presence of a polyaminocyclodextrin, namely the poly-{6-[3-(2-(3-aminopropylamino)-ethylamino)-propylamino]}-(6-deoxy)-b-CD (amCD, figure 1). The obtained Ag-amCD systems, which possess an oniontype structure [1] with a metal core surrounded by several layers of the capping agent, were characterized by means of various complementary techniques. In particular, FT-IR spectroscopy confirmed the presence of the amCD scaffold in the composite, and evidenced a partial oxidative degradation of the polyamine branches,due to the fact that these groups function as sacrificial reducing agents in the photoinduced process of formation of the Ag metal core. TEM and SAED micrographs evidenced that the Ag cores possess a relatively low polydispersity and a significantly crystalline character.Then, in consideration of the well-known antimicrobial activityof nanosized silver, our Ag-amCD systems were assayed for antibacterial activity, quantified as the minimal concentration inhibiting at least the 90% of bacterial growth (MIC90), using Escherichia coli and Kocuria rhizophila as Gram-negative and Gram-positive tester strains. This analysis revealed 5 and 1 μg/ml as MIC90 values against E. coli and K. rhizophila; respectively. In addition, thanks to their peculiar features, the systems function as potential supramolecular drug carriers, effectively able to bind the b-lactam antibiotic Ampicillin (amp, figure 2) as demonstrated by polarimetric measurements. Antimicrobial assays reveals a five-fold improved activity of Ag-amCD-amp probablydue to synergistic action of Agnanoparticles and amp. This studyprovides insights on the attractive possibility to use an environmentally-friendly methodology to produce bioactive supramolecular systemstobeemployedaspowerful andtunableantimicrobial agents. [1] M. Russo, F. Armetta, S. Riela, D. Chillura Martino, P. Lo Meo, R. Noto J. Mol. Cat. A 408 (2015) 250-261.

AB - Biocompatible Ag nanocomposites were prepared by photoreduction of ammoniacal silver acetate in the presence of a polyaminocyclodextrin, namely the poly-{6-[3-(2-(3-aminopropylamino)-ethylamino)-propylamino]}-(6-deoxy)-b-CD (amCD, figure 1). The obtained Ag-amCD systems, which possess an oniontype structure [1] with a metal core surrounded by several layers of the capping agent, were characterized by means of various complementary techniques. In particular, FT-IR spectroscopy confirmed the presence of the amCD scaffold in the composite, and evidenced a partial oxidative degradation of the polyamine branches,due to the fact that these groups function as sacrificial reducing agents in the photoinduced process of formation of the Ag metal core. TEM and SAED micrographs evidenced that the Ag cores possess a relatively low polydispersity and a significantly crystalline character.Then, in consideration of the well-known antimicrobial activityof nanosized silver, our Ag-amCD systems were assayed for antibacterial activity, quantified as the minimal concentration inhibiting at least the 90% of bacterial growth (MIC90), using Escherichia coli and Kocuria rhizophila as Gram-negative and Gram-positive tester strains. This analysis revealed 5 and 1 μg/ml as MIC90 values against E. coli and K. rhizophila; respectively. In addition, thanks to their peculiar features, the systems function as potential supramolecular drug carriers, effectively able to bind the b-lactam antibiotic Ampicillin (amp, figure 2) as demonstrated by polarimetric measurements. Antimicrobial assays reveals a five-fold improved activity of Ag-amCD-amp probablydue to synergistic action of Agnanoparticles and amp. This studyprovides insights on the attractive possibility to use an environmentally-friendly methodology to produce bioactive supramolecular systemstobeemployedaspowerful andtunableantimicrobial agents. [1] M. Russo, F. Armetta, S. Riela, D. Chillura Martino, P. Lo Meo, R. Noto J. Mol. Cat. A 408 (2015) 250-261.

UR - http://hdl.handle.net/10447/177266

M3 - Paper

ER -