SYNTHESIS AND CHARACTERIZATION OF POLYAMINOACIDIC POLYCATIONS FOR GENE DELIVERY

Mariano Licciardi, Gennara Cavallaro, Gaetano Giammona, Melchiorre Cervello, Mariano Licciardi, Gennara Cavallaro, Monica Campisi, Antonina Azzolina, Gaetano Giammona

Risultato della ricerca: Articlepeer review

49 Citazioni (Scopus)

Abstract

The properties as non viral gene vector of a protein-like polymer, the a,b-poly(N-2-hydroxyethyl)-D,L-aspartamide (PHEA) were exploited after its derivatization with 3-(carboxypropyl)trimethyl-ammonium chloride (CPTA) as molecule bearing a cationic group, inorder to obtain stable polycations able to condense DNA. PHEA was firstly functionalized with aminic pendant groups by reaction withethylenediamine (EDA) obtaining the a,b-poly(N-2-hydroxyethyl)(2-aminoethylcarbamate)-D,L-aspartamide (PHEA-EDA) copolymer.We demonstrated that polymer functionalization degree is easily modulable by varying reaction conditions, so allowing to produce two PHEA-EDA derivatives at different molar percentage of amine groups. Subsequently, the condensation reaction of PHEA-EDA copolymers with CPTA yielded a,b-poly(N-2-hydroxyethyl)(2-[3-(trimethylammonium chloride)propylamide]-amidoethylcarbamate)-D,L-aspartamide (PHEA-EDA-CPTA) polycation derivatives.In vitro studies were carried out to evaluate polycations ability to complex DNA and to protect it from nuclease degradation. Obtained results demonstrated the good ability of our new PHEA polycationic derivatives, PHEA-EDA-CPTA, to complex and condense genomic material, neutralizing its anionic charge even at very low polycation/DNA weight ratio. Finally, PHEA-EDA-CPTA polycations were characterized by in vitro cytotoxicity studies to evaluate their effects on the viability of HuH-6 human hepatocellular carcinoma cells by MTS assay. No cytotoxicity was evidenced by both polycationic derivatives after 48 h of incubation at all tested concentrations.
Lingua originaleEnglish
pagine (da-a)2066-2075
Numero di pagine10
RivistaBiomaterials
Volume27
Stato di pubblicazionePublished - 2006

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials

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