Synergistic effects of neurons and astrocytes on the differentiation of brain capillary endothelial cells in culture

Italia Di Liegro, Gabriella Schiera, Giovanni Savettieri, Alessia Gallo, Giovanna Laura Pitarresi, Gabriella Schiera, Epifania Bono, Italia Di Liegro, Maria Pia Raffa, Giovanna Laura Pitarresi

Risultato della ricerca: Article

63 Citazioni (Scopus)

Abstract

Brain capillary endothelial cells form a functional barrier between blood and brain, based on the existence of tight junctions that limit paracellular permeability. Occludin is one of the major transmembrane proteins of tight junctions and its peripheral localization gives indication of tight junction formation. We previously reported that RBE4.B cells (brain capillary endothelial cells), cultured on collagen IV, synthesize occludin and correctly localize it at the cell periphery only when cocultured with neurons. In the present study, we describe a three-cell type-culture system that allowed us to analyze the combined effects of neurons and astrocytes on differentiation of brain capillary endothelial cells in culture. In particular, we found that, in the presence of astrocytes, the neuron-induced synthesis and localization of occludin is precocious as compared to cells cocultured with neurons only.
Lingua originaleEnglish
pagine (da-a)165-170
Numero di pagine6
RivistaJournal of Cellular and Molecular Medicine
Volume7
Stato di pubblicazionePublished - 2003

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Occludin
Astrocytes
Endothelial Cells
Cell Culture Techniques
Neurons
Tight Junctions
Brain
Tight Junction Proteins
Blood-Brain Barrier
Permeability
B-Lymphocytes
Collagen

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Cell Biology

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Synergistic effects of neurons and astrocytes on the differentiation of brain capillary endothelial cells in culture. / Di Liegro, Italia; Schiera, Gabriella; Savettieri, Giovanni; Gallo, Alessia; Pitarresi, Giovanna Laura; Schiera, Gabriella; Bono, Epifania; Di Liegro, Italia; Raffa, Maria Pia; Pitarresi, Giovanna Laura.

In: Journal of Cellular and Molecular Medicine, Vol. 7, 2003, pag. 165-170.

Risultato della ricerca: Article

Di Liegro, Italia ; Schiera, Gabriella ; Savettieri, Giovanni ; Gallo, Alessia ; Pitarresi, Giovanna Laura ; Schiera, Gabriella ; Bono, Epifania ; Di Liegro, Italia ; Raffa, Maria Pia ; Pitarresi, Giovanna Laura. / Synergistic effects of neurons and astrocytes on the differentiation of brain capillary endothelial cells in culture. In: Journal of Cellular and Molecular Medicine. 2003 ; Vol. 7. pagg. 165-170.
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AU - Di Liegro, Italia

AU - Schiera, Gabriella

AU - Savettieri, Giovanni

AU - Gallo, Alessia

AU - Pitarresi, Giovanna Laura

AU - Schiera, Gabriella

AU - Bono, Epifania

AU - Di Liegro, Italia

AU - Raffa, Maria Pia

AU - Pitarresi, Giovanna Laura

PY - 2003

Y1 - 2003

N2 - Brain capillary endothelial cells form a functional barrier between blood and brain, based on the existence of tight junctions that limit paracellular permeability. Occludin is one of the major transmembrane proteins of tight junctions and its peripheral localization gives indication of tight junction formation. We previously reported that RBE4.B cells (brain capillary endothelial cells), cultured on collagen IV, synthesize occludin and correctly localize it at the cell periphery only when cocultured with neurons. In the present study, we describe a three-cell type-culture system that allowed us to analyze the combined effects of neurons and astrocytes on differentiation of brain capillary endothelial cells in culture. In particular, we found that, in the presence of astrocytes, the neuron-induced synthesis and localization of occludin is precocious as compared to cells cocultured with neurons only.

AB - Brain capillary endothelial cells form a functional barrier between blood and brain, based on the existence of tight junctions that limit paracellular permeability. Occludin is one of the major transmembrane proteins of tight junctions and its peripheral localization gives indication of tight junction formation. We previously reported that RBE4.B cells (brain capillary endothelial cells), cultured on collagen IV, synthesize occludin and correctly localize it at the cell periphery only when cocultured with neurons. In the present study, we describe a three-cell type-culture system that allowed us to analyze the combined effects of neurons and astrocytes on differentiation of brain capillary endothelial cells in culture. In particular, we found that, in the presence of astrocytes, the neuron-induced synthesis and localization of occludin is precocious as compared to cells cocultured with neurons only.

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