Study and Characterization of Polyphenol Oxidase from Eggplant (Solanum melongena L.)

Aldo Todaro, Aldo Todaro, Rosalinda Cavallaro, Ferdinando Branca, Giovanni Spagna, Sergio Argento

Risultato della ricerca: Articlepeer review

20 Citazioni (Scopus)


ABSTRACT: In this study the catecholase and cresolase activities of eggplant polyphenol oxidase (PPO) were investigated.Enzyme activity was determined by measuring the increase in absorbance using catechol as substrate and 3-methyl-2-benzothiazolinone hydrazone (MBTH) as coupled reagent. The effects of substrate specificity, heat inactivation, temperature,pH, and inhibitors were investigated to understand the enzymatic alteration of ready-to-eat preparations. Browning of vegetableswas determined through a colorimeter. Decrease of lightness (L*) and increase of color difference values (ΔE*) were correlated withtissue browning. Antibrowning agents were tested on PPO under the same conditions. The enzyme activity was strongly inhibited by0.4Mcitric acid. Under natural pH conditions, the enzyme was also inhibited by tartaric acid and acetic acid. All of the results wereused to understand the best conditions for food transformation (ready-to-eat and grilled eggplant slices).
Lingua originaleEnglish
pagine (da-a)11244-11248
Numero di pagine5
RivistaJournal of Agricultural and Food Chemistry
Stato di pubblicazionePublished - 2011

All Science Journal Classification (ASJC) codes

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  • ???subjectarea.asjc.1100.1100???

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