Structure of rat behavior in hole-board: II) multivariate analysis of modifications induced by diazepam.

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In our previous study we suggested that multivariate analysis could improve hole-board test reliabilityproviding a more useful tool to determine behavioral effects of anxiolytic drugs. To support this hypothesis, amultivariate analysis of rat behavior in hole-board, following administration of the reference anxiolytic drugdiazepam, was carried out. Four groups, each composed of thirty male Wistar rats, were used: one saline andthree diazepam injected (0.25, 0.5 and 2 mg/kg IP). Rat behavior was recorded for 10 min through a digitalvideocamera. Descriptive and multivariate analyses were carried out. In all groups, more than 80% of wholebehavioral structure encompassed walking, climbing, rearing, immobile-sniffing, edge-sniff and head-dip.Moreover, modifications observed of a specific index, represented by edge-sniff/head-dip ratio, werecorrelated to diazepam-induced modifications of anxiety level. Cluster analysis showed that diazepam at 0.5and 2 mg/kg induced important changes for [edge-sniff/head-dip] cluster. In addition, in all diazepam groupsa [walking/climbing] cluster appeared. Path diagrams showed close relationships among different patternsboth in saline and diazepam injected animals. Also, significant changes were detected following diazepam fortransitions encompassing both general exploratory patterns (walking, climbing) and the specific ones (headdipand edge-sniff). Adjusted residuals confirmed in all groups patterns relationships and, where present,significant behavioral associations. Results demonstrate that an anxiolytic activity can be revealed by head-dip/edge-sniff association weakening and by the addressing of behavioral structure toward general exploratoryactivity. Improvement of hole-board test reliability in behavioral study of anxiety, following multivariateanalysis, is emphasized.
Lingua originaleEnglish
pagine (da-a)683-692
Numero di pagine10
Stato di pubblicazionePublished - 2009

All Science Journal Classification (ASJC) codes

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  • ???subjectarea.asjc.2800.2802???


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