Structure and biological evaluation of amino-functionalized PVPnanogels for fast cellular internalization

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Aminopropyl methacrylamide chloride-graft-poly(N-vinyl pyrrolidone) nanogels (NGs) were designed toexploit the favorable properties of poly(N-vinyl pyrrolidone) (PVP), such as its high affinity to water andcomplexation ability of ions, molecules and macromolecules, with the availability of primary aminogroups for bioconjugation reactions. A thorough structural characterization of the nanoscalar networkswas performed via 1H NMR and solid state 13C NMR spectroscopies, while solid state NMR relaxation timemeasurements completed the NGs description in terms of polymer network density. Information on thehydrodynamic size and surface charge densities were sought via dynamic light scattering (DLS) and fpotentialmeasurements. These measurements, carried out both in water and in buffer and at the varianceof pH, temperature and time, support the excellent colloidal stability of the amino-graft PVP nanogels andtheir redispersability from the dry physical form. The total absence of in vitro toxicity and immunogenicitywas ascertained first, and then cell localization studies in cell cultures of fluorescent variants wereperformed. Furthermore, the possibility of decorating the nanogels with bovine serum albumin (BSA)to obtain a stable bio-hybrid colloidal nanoconstruct was established. All the gathered evidences qualifythese materials as potential candidates to be used as nanocarriers for intra-cellular delivery of proteinand other biomolecular drugs.
Lingua originaleEnglish
pagine (da-a)1103-1113
Numero di pagine11
Stato di pubblicazionePublished - 2013

All Science Journal Classification (ASJC) codes

  • ???subjectarea.asjc.1600.1600???
  • ???subjectarea.asjc.2300.2304???
  • ???subjectarea.asjc.1300.1303???
  • ???subjectarea.asjc.1500.1500???
  • ???subjectarea.asjc.2500.2507???
  • ???subjectarea.asjc.2500.2505???


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