Structural characterization of polysaccharides of a productive strain of the culinary-medicinal king oyster mushroom, pleurotus eryngii (Agaricomycetes), from Italy

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Abstract

A preliminary biological investigation of the dry basidiomata of strain C-142-c of Pleurotus eryngii has shown significant antioxidant activity. Two different polysaccharides (PEPS-A1 and PEPS-A2) were isolated from the cultivated edible mushroom, P. eryngii C-142-c strain. Based on acid hydrolysis, methylation analysis, and nuclear magnetic resonance experiments (1H,13C, distortionless enhancement by polarization transfer, double quantum filtered correlation spectroscopy, total correlation spectroscopy, nuclear Overhauser effect spectroscopy, heteronuclear single-quantum correlation spectroscopy, and heteronuclear multiple-bond correlation spectroscopy), the structures of the repeating unit of PEPS-A1 and PEPS-A2 were established as follows: (1) PEPS-A1 (α-glucan): [→6)-α-D-Glcp-(1→6)-α-D-Glcp-(1→]n; and (2) PEPS-A2 (β-glucan): [→6)-β-D-Glcp-(1→6)-β-D-Glcp-(1→]n. The antioxidant activity of PEPS-A1 and PEPS-A2 was evaluated as hydroxyl radical scavenging activity. PEPS-A1 and PEPS-A2 showed SC50 values of 400 µg/mL and 122 µg/mL, respectively, suggesting their possible use as a dietary supplement in functional foods. The polysaccharides were tested for their activity on cell viability using a colorectal adenocarcinoma cell line (HT-29). Both polysaccharides affected cell viability after 48 and 72 hours of treatment, inducing the death of 50% of HT-29 cells between 0.25 and 1 µg/mL and between 0.5 and 1 µg/mL, respectively, for PEPS-A1 and PEPS-A2. These results are promising for future applications of these mushroom-derived polysaccharides as antioxidants and antitumor agents.
Lingua originaleEnglish
pagine (da-a)717-726
Numero di pagine10
RivistaInternational Journal of Medicinal Mushrooms
Volume20
Stato di pubblicazionePublished - 2018

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Pleurotus
varespladib methyl
Italy
Polysaccharides
Spectrum Analysis
Antioxidants
Agaricales
Cell Survival
HT29 Cells
Functional Food
Dietary Supplements
Hydroxyl Radical
Antineoplastic Agents
Methylation
Adenocarcinoma
Hydrolysis
Magnetic Resonance Spectroscopy
Cell Line
Acids

All Science Journal Classification (ASJC) codes

  • Applied Microbiology and Biotechnology
  • Pharmacology
  • Drug Discovery

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@article{5f0b010a6cc748118fd7ca64f3a6f164,
title = "Structural characterization of polysaccharides of a productive strain of the culinary-medicinal king oyster mushroom, pleurotus eryngii (Agaricomycetes), from Italy",
abstract = "A preliminary biological investigation of the dry basidiomata of strain C-142-c of Pleurotus eryngii has shown significant antioxidant activity. Two different polysaccharides (PEPS-A1 and PEPS-A2) were isolated from the cultivated edible mushroom, P. eryngii C-142-c strain. Based on acid hydrolysis, methylation analysis, and nuclear magnetic resonance experiments (1H,13C, distortionless enhancement by polarization transfer, double quantum filtered correlation spectroscopy, total correlation spectroscopy, nuclear Overhauser effect spectroscopy, heteronuclear single-quantum correlation spectroscopy, and heteronuclear multiple-bond correlation spectroscopy), the structures of the repeating unit of PEPS-A1 and PEPS-A2 were established as follows: (1) PEPS-A1 (α-glucan): [→6)-α-D-Glcp-(1→6)-α-D-Glcp-(1→]n; and (2) PEPS-A2 (β-glucan): [→6)-β-D-Glcp-(1→6)-β-D-Glcp-(1→]n. The antioxidant activity of PEPS-A1 and PEPS-A2 was evaluated as hydroxyl radical scavenging activity. PEPS-A1 and PEPS-A2 showed SC50 values of 400 µg/mL and 122 µg/mL, respectively, suggesting their possible use as a dietary supplement in functional foods. The polysaccharides were tested for their activity on cell viability using a colorectal adenocarcinoma cell line (HT-29). Both polysaccharides affected cell viability after 48 and 72 hours of treatment, inducing the death of 50{\%} of HT-29 cells between 0.25 and 1 µg/mL and between 0.5 and 1 µg/mL, respectively, for PEPS-A1 and PEPS-A2. These results are promising for future applications of these mushroom-derived polysaccharides as antioxidants and antitumor agents.",
author = "Giuseppe Bonaventura and Gargano, {Maria Letizia} and Giuseppe Venturella and {Caruso Bavisotto}, Celeste and Roberta Calvo and Marina Zacchigna and Giuseppe Procida and Francesca Cateni",
year = "2018",
language = "English",
volume = "20",
pages = "717--726",
journal = "International Journal of Medicinal Mushrooms",
issn = "1521-9437",
publisher = "Begell House Inc.",

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TY - JOUR

T1 - Structural characterization of polysaccharides of a productive strain of the culinary-medicinal king oyster mushroom, pleurotus eryngii (Agaricomycetes), from Italy

AU - Bonaventura, Giuseppe

AU - Gargano, Maria Letizia

AU - Venturella, Giuseppe

AU - Caruso Bavisotto, Celeste

AU - Calvo, Roberta

AU - Zacchigna, Marina

AU - Procida, Giuseppe

AU - Cateni, Francesca

PY - 2018

Y1 - 2018

N2 - A preliminary biological investigation of the dry basidiomata of strain C-142-c of Pleurotus eryngii has shown significant antioxidant activity. Two different polysaccharides (PEPS-A1 and PEPS-A2) were isolated from the cultivated edible mushroom, P. eryngii C-142-c strain. Based on acid hydrolysis, methylation analysis, and nuclear magnetic resonance experiments (1H,13C, distortionless enhancement by polarization transfer, double quantum filtered correlation spectroscopy, total correlation spectroscopy, nuclear Overhauser effect spectroscopy, heteronuclear single-quantum correlation spectroscopy, and heteronuclear multiple-bond correlation spectroscopy), the structures of the repeating unit of PEPS-A1 and PEPS-A2 were established as follows: (1) PEPS-A1 (α-glucan): [→6)-α-D-Glcp-(1→6)-α-D-Glcp-(1→]n; and (2) PEPS-A2 (β-glucan): [→6)-β-D-Glcp-(1→6)-β-D-Glcp-(1→]n. The antioxidant activity of PEPS-A1 and PEPS-A2 was evaluated as hydroxyl radical scavenging activity. PEPS-A1 and PEPS-A2 showed SC50 values of 400 µg/mL and 122 µg/mL, respectively, suggesting their possible use as a dietary supplement in functional foods. The polysaccharides were tested for their activity on cell viability using a colorectal adenocarcinoma cell line (HT-29). Both polysaccharides affected cell viability after 48 and 72 hours of treatment, inducing the death of 50% of HT-29 cells between 0.25 and 1 µg/mL and between 0.5 and 1 µg/mL, respectively, for PEPS-A1 and PEPS-A2. These results are promising for future applications of these mushroom-derived polysaccharides as antioxidants and antitumor agents.

AB - A preliminary biological investigation of the dry basidiomata of strain C-142-c of Pleurotus eryngii has shown significant antioxidant activity. Two different polysaccharides (PEPS-A1 and PEPS-A2) were isolated from the cultivated edible mushroom, P. eryngii C-142-c strain. Based on acid hydrolysis, methylation analysis, and nuclear magnetic resonance experiments (1H,13C, distortionless enhancement by polarization transfer, double quantum filtered correlation spectroscopy, total correlation spectroscopy, nuclear Overhauser effect spectroscopy, heteronuclear single-quantum correlation spectroscopy, and heteronuclear multiple-bond correlation spectroscopy), the structures of the repeating unit of PEPS-A1 and PEPS-A2 were established as follows: (1) PEPS-A1 (α-glucan): [→6)-α-D-Glcp-(1→6)-α-D-Glcp-(1→]n; and (2) PEPS-A2 (β-glucan): [→6)-β-D-Glcp-(1→6)-β-D-Glcp-(1→]n. The antioxidant activity of PEPS-A1 and PEPS-A2 was evaluated as hydroxyl radical scavenging activity. PEPS-A1 and PEPS-A2 showed SC50 values of 400 µg/mL and 122 µg/mL, respectively, suggesting their possible use as a dietary supplement in functional foods. The polysaccharides were tested for their activity on cell viability using a colorectal adenocarcinoma cell line (HT-29). Both polysaccharides affected cell viability after 48 and 72 hours of treatment, inducing the death of 50% of HT-29 cells between 0.25 and 1 µg/mL and between 0.5 and 1 µg/mL, respectively, for PEPS-A1 and PEPS-A2. These results are promising for future applications of these mushroom-derived polysaccharides as antioxidants and antitumor agents.

UR - http://hdl.handle.net/10447/353429

UR - http://dl.begellhouse.com/download/article/3818d4963fba2ee9/IJM2007-02-27011 revised proof 3.pdf

M3 - Article

VL - 20

SP - 717

EP - 726

JO - International Journal of Medicinal Mushrooms

JF - International Journal of Medicinal Mushrooms

SN - 1521-9437

ER -