TY - CONF
T1 - Streptomyces coelicolor secretoma protects astrocytes from oxidative stress
AU - Caltabellotta, N.
AU - Puglia, Anna Maria
AU - Di Liegro, Italia
AU - Schiera, Gabriella
AU - Di Liegro, Carlo Maria
AU - Faddetta, Teresa
AU - Fricano, Anna
PY - 2016
Y1 - 2016
N2 - Streptomycetes are gram-positive bacteria that produce about 2/3 of pharmaceutically active secondary
metabolites, such as antibiotics, and anti-tumor, immunosuppressive, antifungal and antiparasitic agents. In
this study, we investigated the possible effects of Streptomyces coelicolor extracts and putative vesicular
fraction on primary cultures of rat astrocytes, in both physiological and stressed conditions, induced by
treatment with hydrogen peroxide. Briefly, crude extracts and putative vesicular fractions were prepared
from two S.coelicolor strains (M145 wild-type strain and bold F166 strain), and used to treat primary
astrocytes, which were then also treated with hydrogen peroxide. Data obtained show that both the general
protein pattern of S.coelicolor and putative vesicular fraction vary at different times of growth. M145 strain
vesicles and the crude extracts from both strains have a protective effect on stressed astrocytes. The
protective effects are higher with the vesicular fraction from bacteria at 18 and 72h of growth. The 166 strain
crude extract has a greater protective effect than M145 strain, especially in the initial stages of growth: 18h
and 24h.
Although they need further studies, these observations are very promising and suggest that, in the future,
molecules produced by these bacteria may offer a protection against the high ROS production observed in
many pathological conditions, such as in neurodegenerative diseases.
AB - Streptomycetes are gram-positive bacteria that produce about 2/3 of pharmaceutically active secondary
metabolites, such as antibiotics, and anti-tumor, immunosuppressive, antifungal and antiparasitic agents. In
this study, we investigated the possible effects of Streptomyces coelicolor extracts and putative vesicular
fraction on primary cultures of rat astrocytes, in both physiological and stressed conditions, induced by
treatment with hydrogen peroxide. Briefly, crude extracts and putative vesicular fractions were prepared
from two S.coelicolor strains (M145 wild-type strain and bold F166 strain), and used to treat primary
astrocytes, which were then also treated with hydrogen peroxide. Data obtained show that both the general
protein pattern of S.coelicolor and putative vesicular fraction vary at different times of growth. M145 strain
vesicles and the crude extracts from both strains have a protective effect on stressed astrocytes. The
protective effects are higher with the vesicular fraction from bacteria at 18 and 72h of growth. The 166 strain
crude extract has a greater protective effect than M145 strain, especially in the initial stages of growth: 18h
and 24h.
Although they need further studies, these observations are very promising and suggest that, in the future,
molecules produced by these bacteria may offer a protection against the high ROS production observed in
many pathological conditions, such as in neurodegenerative diseases.
KW - Streptomyces coelicolor, primary cultures of rat astrocytes, utative vesicular fraction, ROS production
UR - http://hdl.handle.net/10447/249523
M3 - Paper
ER -