TY - JOUR
T1 - Strategies for the production of difficult-to-express full-length eukaryotic proteins using microbial cell factories: production of human alpha-galactosidase A
AU - Giuliani Ricci, Anna Maria
AU - Accardi, Giulia
AU - Vázquez, Felicitas
AU - Unzueta, Ugutz
AU - Toledo-Rubio, Verónica
AU - Mendoza, Rosa
AU - Accardi, Giulia
AU - Giuliani, Maria
AU - Abasolo, Ibane
AU - Ferrer-Miralles, Neus
AU - Corchero, José L.
AU - Sannino, Filomena
AU - Villaverde, Antonio
AU - Schwartz, Simo
PY - 2015
Y1 - 2015
N2 - Obtaining high levels of pure proteins remains the main bottleneck of many scientific and biotechnological studies. Among all the available recombinant expression systems, Escherichia coli facilitates gene expression by its relative simplicity, inexpensive and fast cultivation, well-known genetics and the large number of tools available for its biotechnological application. However, recombinant expression in E. coli is not always a straightforward procedure and major obstacles are encountered when producing many eukaryotic proteins and especially membrane proteins, linked to missing posttranslational modifications, proteolysis and aggregation. In this context, many conventional and unconventional eukaryotic hosts are under exploration and development, but in some cases linked to complex culture media or processes. In this context, alternative bacterial systems able to overcome some of the limitations posed by E. coli keeping the simplicity of prokaryotic manipulation are currently emerging as convenient hosts for protein production. We have comparatively produced a “difficult-to-express” human protein, the lysosomal enzyme alpha-galactosidase A (hGLA) in E. coli and in the psychrophilic bacterium Pseudoalteromonas haloplanktis TAC125 cells (P. haloplanktis TAC125). While in E. coli the production of active hGLA was unreachable due to proteolytic instability and/or protein misfolding, the expression of hGLA gene in P. haloplanktis TAC125 allows obtaining active enzyme. These results are discussed in the context of emerging bacterial systems for protein production that represent appealing alternatives to the regular use of E. coli and also of more complex eukaryotic systems.
AB - Obtaining high levels of pure proteins remains the main bottleneck of many scientific and biotechnological studies. Among all the available recombinant expression systems, Escherichia coli facilitates gene expression by its relative simplicity, inexpensive and fast cultivation, well-known genetics and the large number of tools available for its biotechnological application. However, recombinant expression in E. coli is not always a straightforward procedure and major obstacles are encountered when producing many eukaryotic proteins and especially membrane proteins, linked to missing posttranslational modifications, proteolysis and aggregation. In this context, many conventional and unconventional eukaryotic hosts are under exploration and development, but in some cases linked to complex culture media or processes. In this context, alternative bacterial systems able to overcome some of the limitations posed by E. coli keeping the simplicity of prokaryotic manipulation are currently emerging as convenient hosts for protein production. We have comparatively produced a “difficult-to-express” human protein, the lysosomal enzyme alpha-galactosidase A (hGLA) in E. coli and in the psychrophilic bacterium Pseudoalteromonas haloplanktis TAC125 cells (P. haloplanktis TAC125). While in E. coli the production of active hGLA was unreachable due to proteolytic instability and/or protein misfolding, the expression of hGLA gene in P. haloplanktis TAC125 allows obtaining active enzyme. These results are discussed in the context of emerging bacterial systems for protein production that represent appealing alternatives to the regular use of E. coli and also of more complex eukaryotic systems.
KW - Applied Microbiology and Biotechnology
KW - Biotechnology
KW - Enzyme Stability
KW - Escherichia coli
KW - Expression systems
KW - Fabry’s disease
KW - Human alpha-galactosidase A
KW - Humans
KW - Metabolic Engineering
KW - Pseudoalteromonas
KW - Pseudoalteromonas haloplanktis TAC125
KW - Recombinant Proteins
KW - Recombinant protein
KW - alpha-Galactosidase
KW - Applied Microbiology and Biotechnology
KW - Biotechnology
KW - Enzyme Stability
KW - Escherichia coli
KW - Expression systems
KW - Fabry’s disease
KW - Human alpha-galactosidase A
KW - Humans
KW - Metabolic Engineering
KW - Pseudoalteromonas
KW - Pseudoalteromonas haloplanktis TAC125
KW - Recombinant Proteins
KW - Recombinant protein
KW - alpha-Galactosidase
UR - http://hdl.handle.net/10447/295073
M3 - Article
VL - 99
SP - 5863
EP - 5874
JO - Applied Microbiology and Biotechnology
JF - Applied Microbiology and Biotechnology
SN - 0175-7598
ER -