Spin trapping as tool for investigating radiation induced free radicals in biologically active molecules

Risultato della ricerca: Other

Abstract

Since from the second half of the last century the spin trapping has found wide applications inchemistry, biology and medicine [1]. Of particular interest are the studied of the free radicals inducedin biologically active molecules, such as DNA and proteins, when they are exposed to ionizingradiation. Their characterization allows to investigate the radiation-induced primary processes insideliving cells. Indeed, ionizing radiations produce many kinds of free radicals through direct action;radicals can be formed also by indirect actions. However, the direct detection of these radicals is hardto obtain as they have a too short lifetime and this makes difficult to record them by conventionalelectron spin resonance (ESR) technique. This limitation can be overcome by means of spin-trappingmethod wherein these short-lived free radicals are converted using a diamagnetic scavenger (thespin trap) to longer-lived radicals (the spin adducts), which can be conveniently investigated by meansof ESR.Here, we review a combined method of ESR and spin trapping to identify free radicals induced bydirect ionization as well as OH-radical reactions in nucleic-acid related and protein-relatedcompounds. ESR combined with spin trapping remains a useful method for clarifying these processesin a living organism.
Lingua originaleEnglish
Numero di pagine1
Stato di pubblicazionePublished - 2017

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