Spectrophotometric evidence for the solubilization site of betalain pigments in membrane biomimetic systems

Luisa Tesoriere, Maria Antonia Livrea, Renato Lombardo, Luciana Sciascia, Maria Liria Turco Liveri, Maria Liria Turco Liveri, Luciana Sciascia, Renato Lombardo

Risultato della ricerca: Articlepeer review

17 Citazioni (Scopus)


The solubilization site of two betalain pigments, namely, betanin and indicaxantin, into L-α-dipalmitoylphosphatidylcholine (DPPC) and dimyristoylphosphatidylcholine (DMPC) vesicles was investigated by a spectrophotometric study. Pigment absorbance was monitored by varying phospholipid concentration, at a constant temperature that was varied in a range including the main phase transition temperature (Tm) of the relevant phospholipid bilayer. Maximum betanin absorption increased with the increase of DPPC concentration within the entire temperature range, reaching a plateau. The binding constant (Kb) of the pigment, calculated according to a pseudo-two-phase model, varied with the temperature, indicating that betanin is located at the hydrophobic interior of the bilayer. Other measurements of binding of betanin to DMPC and of indicaxanthin to either DPPC or DMPC vesicles ruled out that these compounds were solubilized in the hydrophobic interior of these bilayers.
Lingua originaleEnglish
pagine (da-a)2836-2840
Numero di pagine5
RivistaJournal of Agricultural and Food Chemistry
Stato di pubblicazionePublished - 2007

All Science Journal Classification (ASJC) codes

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  • ???subjectarea.asjc.1100.1100???

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