Somatic embryogenesis and plant regeneration from pistil transverse thin cell layers of lemon (Citrus limon).

Maurizio Sajeva, Francesco Carimi, Angela Carra, Fabio De Pasquale

Risultato della ricerca: Article

2 Citazioni (Scopus)

Abstract

Callus induction, somatic embryogenesis and plant regeneration were obtained in Citrus limon (L.) Burm. (cv. Femminello) from cultures of pistil transverse thin cell layer explants [(t)TCLs]. Explants were cultured on two different media, based on Murashige and Skoog salts and vitamins, supplemented with 500 mg l−1 malt extract (MSI), or 500 mg l−1 malt extract and 13.3 μM 6-benzylaminopurine (MSII). Sucrose (146 mM) was used as carbon source. Somatic embryos appeared 3 months after culture initiation from stigma and style (t)TCLs; they were observed at the surface of the (t)TCL-derived callus. Although ovary (t)TCLs showed the highest callus formation, they never differentiated somatic embryos. Percentages of embryo formation from (t)TCLs incubated on MSI (13% and 2% for stigma and style, respectively) were lower than those from (t)TCLs incubated on MSII (36% and 7% for stigma and style, respectively). The embryogenic response of stigma (t)TCLs was usually higher than that of style (t)TCLs. After about 3 months, somatic embryos developed into plantlets at high frequencies (57% and 62% for stigma- and style-derived somatic embryos, respectively).
Lingua originaleEnglish
pagine (da-a)199-203
RivistaPLANT BIOSYSTEMS
Volume142
Stato di pubblicazionePublished - 2008

Fingerprint

Citrus limon
pistil
lemons
somatic embryogenesis
stigma
embryo
somatic embryos
regeneration
malt extract
cells
explants
callus
callus formation
vitamin
sucrose
embryo (plant)
benzyladenine
plantlets
vitamins
embryogenesis

All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics
  • Plant Science

Cita questo

Somatic embryogenesis and plant regeneration from pistil transverse thin cell layers of lemon (Citrus limon). / Sajeva, Maurizio; Carimi, Francesco; Carra, Angela; De Pasquale, Fabio.

In: PLANT BIOSYSTEMS, Vol. 142, 2008, pag. 199-203.

Risultato della ricerca: Article

Sajeva, Maurizio ; Carimi, Francesco ; Carra, Angela ; De Pasquale, Fabio. / Somatic embryogenesis and plant regeneration from pistil transverse thin cell layers of lemon (Citrus limon). In: PLANT BIOSYSTEMS. 2008 ; Vol. 142. pagg. 199-203.
@article{449cfbae795d4cb9b8ea167d505b62e1,
title = "Somatic embryogenesis and plant regeneration from pistil transverse thin cell layers of lemon (Citrus limon).",
abstract = "Callus induction, somatic embryogenesis and plant regeneration were obtained in Citrus limon (L.) Burm. (cv. Femminello) from cultures of pistil transverse thin cell layer explants [(t)TCLs]. Explants were cultured on two different media, based on Murashige and Skoog salts and vitamins, supplemented with 500 mg l−1 malt extract (MSI), or 500 mg l−1 malt extract and 13.3 μM 6-benzylaminopurine (MSII). Sucrose (146 mM) was used as carbon source. Somatic embryos appeared 3 months after culture initiation from stigma and style (t)TCLs; they were observed at the surface of the (t)TCL-derived callus. Although ovary (t)TCLs showed the highest callus formation, they never differentiated somatic embryos. Percentages of embryo formation from (t)TCLs incubated on MSI (13{\%} and 2{\%} for stigma and style, respectively) were lower than those from (t)TCLs incubated on MSII (36{\%} and 7{\%} for stigma and style, respectively). The embryogenic response of stigma (t)TCLs was usually higher than that of style (t)TCLs. After about 3 months, somatic embryos developed into plantlets at high frequencies (57{\%} and 62{\%} for stigma- and style-derived somatic embryos, respectively).",
author = "Maurizio Sajeva and Francesco Carimi and Angela Carra and {De Pasquale}, Fabio",
year = "2008",
language = "English",
volume = "142",
pages = "199--203",
journal = "PLANT BIOSYSTEMS",
issn = "1126-3504",

}

TY - JOUR

T1 - Somatic embryogenesis and plant regeneration from pistil transverse thin cell layers of lemon (Citrus limon).

AU - Sajeva, Maurizio

AU - Carimi, Francesco

AU - Carra, Angela

AU - De Pasquale, Fabio

PY - 2008

Y1 - 2008

N2 - Callus induction, somatic embryogenesis and plant regeneration were obtained in Citrus limon (L.) Burm. (cv. Femminello) from cultures of pistil transverse thin cell layer explants [(t)TCLs]. Explants were cultured on two different media, based on Murashige and Skoog salts and vitamins, supplemented with 500 mg l−1 malt extract (MSI), or 500 mg l−1 malt extract and 13.3 μM 6-benzylaminopurine (MSII). Sucrose (146 mM) was used as carbon source. Somatic embryos appeared 3 months after culture initiation from stigma and style (t)TCLs; they were observed at the surface of the (t)TCL-derived callus. Although ovary (t)TCLs showed the highest callus formation, they never differentiated somatic embryos. Percentages of embryo formation from (t)TCLs incubated on MSI (13% and 2% for stigma and style, respectively) were lower than those from (t)TCLs incubated on MSII (36% and 7% for stigma and style, respectively). The embryogenic response of stigma (t)TCLs was usually higher than that of style (t)TCLs. After about 3 months, somatic embryos developed into plantlets at high frequencies (57% and 62% for stigma- and style-derived somatic embryos, respectively).

AB - Callus induction, somatic embryogenesis and plant regeneration were obtained in Citrus limon (L.) Burm. (cv. Femminello) from cultures of pistil transverse thin cell layer explants [(t)TCLs]. Explants were cultured on two different media, based on Murashige and Skoog salts and vitamins, supplemented with 500 mg l−1 malt extract (MSI), or 500 mg l−1 malt extract and 13.3 μM 6-benzylaminopurine (MSII). Sucrose (146 mM) was used as carbon source. Somatic embryos appeared 3 months after culture initiation from stigma and style (t)TCLs; they were observed at the surface of the (t)TCL-derived callus. Although ovary (t)TCLs showed the highest callus formation, they never differentiated somatic embryos. Percentages of embryo formation from (t)TCLs incubated on MSI (13% and 2% for stigma and style, respectively) were lower than those from (t)TCLs incubated on MSII (36% and 7% for stigma and style, respectively). The embryogenic response of stigma (t)TCLs was usually higher than that of style (t)TCLs. After about 3 months, somatic embryos developed into plantlets at high frequencies (57% and 62% for stigma- and style-derived somatic embryos, respectively).

UR - http://hdl.handle.net/10447/21898

M3 - Article

VL - 142

SP - 199

EP - 203

JO - PLANT BIOSYSTEMS

JF - PLANT BIOSYSTEMS

SN - 1126-3504

ER -