SiPM as miniaturised optical biosensor for DNA-microarray applications

Maria Francesca Santangelo, Alessandro Busacca, Emanuele Luigi Sciuto, Maria Francesca Santangelo, Petralia, Sabrina Conoci, Libertino, Salvatore Petralia

Risultato della ricerca: Articlepeer review

15 Citazioni (Scopus)

Abstract

A miniaturized optical biosensor for low-level fluorescence emitted by DNA strands labelled with CY5 is showed. Aim of this work is to demonstrate that a Si-based photodetector, having a low noise and a high sensitivity, can replace traditional detection systems in DNA-microarray applications. The photodetector used is a photomultiplier (SiPM), with 25. pixels. It exhibits a higher sensitivity than commercial optical readers and we experimentally found a detection limit for spotted dried samples of ~1 nM. We measured the fluorescence signal in different operating conditions (angle of analysis, fluorophores concentrations, solution volumes and support). Once fixed the angle of analysis, for samples spotted on Al-TEOS slide dried, the system is proportional to the concentration of the analyte in the sample and is linear in the range 1. nM-1. μM. For solutions, the range of linearity ranges from 100. fM to 10. nM. The system potentialities and the device low costs suggest it as basic component for the design and fabrication of a cheap, easy and portable optical system.
Lingua originaleEnglish
pagine (da-a)95-98
Numero di pagine4
RivistaSensing and Bio-Sensing Research
Volume6
Stato di pubblicazionePublished - 2015

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Electronic, Optical and Magnetic Materials
  • Signal Processing
  • Electrical and Electronic Engineering

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