Senescence-associated HSP60 expression in Normal Human skin fibroblasts

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Abstract

Normal mammalian fibroblasts cultured in vitro undergo a limited number of divisions before entering a senescent phase in which they can be maintained for long periods but cannot be induced to divide. Senescent cells become unresponsive to growth-promoting signals and exhibit senescent cell morphology with flattened and enlarged cell shape. Several chaperones have a direct effect on cellular senescence. HSP60 has been largely studied in our laboratories and it has been associated with uncontrolled cell proliferation in tumor cells. Since senescence is firmly regulated during cell cycle progression, we wanted to investigate HSP60 protein level during cellular senescence. Our data show that HSP60 increases during the initial stage of senescence and that it is localized in cellular compartments, resembling mitochondria. An increase in HSP60 protein amount is associated with a cell cycle slow-down and it may have a role in cell cycle progression.
Lingua originaleEnglish
pagine (da-a)446-453
Numero di pagine8
RivistaANATOMICAL RECORD. PART A, DISCOVERIES IN MOLECULAR, CELLULAR, AND EVOLUTIONARY BIOLOGY
Volume284
Stato di pubblicazionePublished - 2005

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skin (animal)
fibroblasts
cell cycle
Cell Cycle
Fibroblasts
Cell Aging
Skin
Cell Shape
cells
cell proliferation
Mitochondria
Proteins
mitochondria
proteins
Cell Proliferation
Growth
Neoplasms
cell senescence

All Science Journal Classification (ASJC) codes

  • Anatomy
  • Agricultural and Biological Sciences (miscellaneous)

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title = "Senescence-associated HSP60 expression in Normal Human skin fibroblasts",
abstract = "Normal mammalian fibroblasts cultured in vitro undergo a limited number of divisions before entering a senescent phase in which they can be maintained for long periods but cannot be induced to divide. Senescent cells become unresponsive to growth-promoting signals and exhibit senescent cell morphology with flattened and enlarged cell shape. Several chaperones have a direct effect on cellular senescence. HSP60 has been largely studied in our laboratories and it has been associated with uncontrolled cell proliferation in tumor cells. Since senescence is firmly regulated during cell cycle progression, we wanted to investigate HSP60 protein level during cellular senescence. Our data show that HSP60 increases during the initial stage of senescence and that it is localized in cellular compartments, resembling mitochondria. An increase in HSP60 protein amount is associated with a cell cycle slow-down and it may have a role in cell cycle progression.",
author = "Giovanni Zummo and Felicia Farina and Francesco Cappello and {Di Felice}, Valentina and Ardizzone, {Nella Maria} and {Di Felice}, Valentina",
year = "2005",
language = "English",
volume = "284",
pages = "446--453",
journal = "Anatomical Record - Part A Discoveries in Molecular, Cellular, and Evolutionary Biology",
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T1 - Senescence-associated HSP60 expression in Normal Human skin fibroblasts

AU - Zummo, Giovanni

AU - Farina, Felicia

AU - Cappello, Francesco

AU - Di Felice, Valentina

AU - Ardizzone, Nella Maria

AU - Di Felice, Valentina

PY - 2005

Y1 - 2005

N2 - Normal mammalian fibroblasts cultured in vitro undergo a limited number of divisions before entering a senescent phase in which they can be maintained for long periods but cannot be induced to divide. Senescent cells become unresponsive to growth-promoting signals and exhibit senescent cell morphology with flattened and enlarged cell shape. Several chaperones have a direct effect on cellular senescence. HSP60 has been largely studied in our laboratories and it has been associated with uncontrolled cell proliferation in tumor cells. Since senescence is firmly regulated during cell cycle progression, we wanted to investigate HSP60 protein level during cellular senescence. Our data show that HSP60 increases during the initial stage of senescence and that it is localized in cellular compartments, resembling mitochondria. An increase in HSP60 protein amount is associated with a cell cycle slow-down and it may have a role in cell cycle progression.

AB - Normal mammalian fibroblasts cultured in vitro undergo a limited number of divisions before entering a senescent phase in which they can be maintained for long periods but cannot be induced to divide. Senescent cells become unresponsive to growth-promoting signals and exhibit senescent cell morphology with flattened and enlarged cell shape. Several chaperones have a direct effect on cellular senescence. HSP60 has been largely studied in our laboratories and it has been associated with uncontrolled cell proliferation in tumor cells. Since senescence is firmly regulated during cell cycle progression, we wanted to investigate HSP60 protein level during cellular senescence. Our data show that HSP60 increases during the initial stage of senescence and that it is localized in cellular compartments, resembling mitochondria. An increase in HSP60 protein amount is associated with a cell cycle slow-down and it may have a role in cell cycle progression.

UR - http://hdl.handle.net/10447/16699

M3 - Article

VL - 284

SP - 446

EP - 453

JO - Anatomical Record - Part A Discoveries in Molecular, Cellular, and Evolutionary Biology

JF - Anatomical Record - Part A Discoveries in Molecular, Cellular, and Evolutionary Biology

SN - 1552-4884

ER -