Seeding nerve sutures with minced nerve-graft (MINE-G): a simple method to improve nerve regeneration in rats

Francesco Moschella, Anna Martorana, Giovanni Cassata, Carla Cannizzaro, Salvatore D'Arpa, Adriana Cordova, Fulvio Plescia, Giovanni Zabbia, Giovanni Cassata, Roberto Puleio, Luca Cicero, Grazia Salimbeni, Salvatore D’Arpa

Risultato della ricerca: Article

Abstract

Background: The aim of this study was to assess the effect of seeding the distal nerve suture with nerve fragments in rats. Methods: On 20 rats, a 15 mm sciatic nerve defect was reconstructed with a nerve autograft. In the Study Group (10 rats), a minced 1 mm nerve segment was seeded around the nerve suture. In the Control Group (10 rats), a nerve graft alone was used. At 4 and 12 weeks, a walking track analysis with open field test (WTA), hystomorphometry (number of myelinated fibers (n), fiber density (FD) and fiber area (FA) and soleus and gastrocnemius muscle weight ratios (MWR) were evaluated. The Student t-test was used for statistical analysis. Results: At 4 and 12 weeks the Study Group had a significantly higher n and FD (p =.043 and.033). The SMWR was significantly higher in the Study Group at 12 weeks (p =.0207). Conclusions: Seeding the distal nerve suture with nerve fragments increases the number of myelinated fibers, the FD and the SMWR. The technique seems promising and deserves further investigation to clarify the mechanisms involved and its functional effects.
Lingua originaleEnglish
pagine (da-a)27-35
Numero di pagine9
RivistaDefault journal
Volume118
Stato di pubblicazionePublished - 2018

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Nerve Regeneration
Sutures
Transplants
Skeletal Muscle
Autografts
Sciatic Nerve
Walking
Students
Weights and Measures
Control Groups

All Science Journal Classification (ASJC) codes

  • Surgery

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title = "Seeding nerve sutures with minced nerve-graft (MINE-G): a simple method to improve nerve regeneration in rats",
abstract = "Background: The aim of this study was to assess the effect of seeding the distal nerve suture with nerve fragments in rats. Methods: On 20 rats, a 15 mm sciatic nerve defect was reconstructed with a nerve autograft. In the Study Group (10 rats), a minced 1 mm nerve segment was seeded around the nerve suture. In the Control Group (10 rats), a nerve graft alone was used. At 4 and 12 weeks, a walking track analysis with open field test (WTA), hystomorphometry (number of myelinated fibers (n), fiber density (FD) and fiber area (FA) and soleus and gastrocnemius muscle weight ratios (MWR) were evaluated. The Student t-test was used for statistical analysis. Results: At 4 and 12 weeks the Study Group had a significantly higher n and FD (p =.043 and.033). The SMWR was significantly higher in the Study Group at 12 weeks (p =.0207). Conclusions: Seeding the distal nerve suture with nerve fragments increases the number of myelinated fibers, the FD and the SMWR. The technique seems promising and deserves further investigation to clarify the mechanisms involved and its functional effects.",
author = "Francesco Moschella and Anna Martorana and Giovanni Cassata and Carla Cannizzaro and Salvatore D'Arpa and Adriana Cordova and Fulvio Plescia and Giovanni Zabbia and Giovanni Cassata and Roberto Puleio and Luca Cicero and Grazia Salimbeni and Salvatore D’Arpa",
year = "2018",
language = "English",
volume = "118",
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TY - JOUR

T1 - Seeding nerve sutures with minced nerve-graft (MINE-G): a simple method to improve nerve regeneration in rats

AU - Moschella, Francesco

AU - Martorana, Anna

AU - Cassata, Giovanni

AU - Cannizzaro, Carla

AU - D'Arpa, Salvatore

AU - Cordova, Adriana

AU - Plescia, Fulvio

AU - Zabbia, Giovanni

AU - Cassata, Giovanni

AU - Puleio, Roberto

AU - Cicero, Luca

AU - Salimbeni, Grazia

AU - D’Arpa, Salvatore

PY - 2018

Y1 - 2018

N2 - Background: The aim of this study was to assess the effect of seeding the distal nerve suture with nerve fragments in rats. Methods: On 20 rats, a 15 mm sciatic nerve defect was reconstructed with a nerve autograft. In the Study Group (10 rats), a minced 1 mm nerve segment was seeded around the nerve suture. In the Control Group (10 rats), a nerve graft alone was used. At 4 and 12 weeks, a walking track analysis with open field test (WTA), hystomorphometry (number of myelinated fibers (n), fiber density (FD) and fiber area (FA) and soleus and gastrocnemius muscle weight ratios (MWR) were evaluated. The Student t-test was used for statistical analysis. Results: At 4 and 12 weeks the Study Group had a significantly higher n and FD (p =.043 and.033). The SMWR was significantly higher in the Study Group at 12 weeks (p =.0207). Conclusions: Seeding the distal nerve suture with nerve fragments increases the number of myelinated fibers, the FD and the SMWR. The technique seems promising and deserves further investigation to clarify the mechanisms involved and its functional effects.

AB - Background: The aim of this study was to assess the effect of seeding the distal nerve suture with nerve fragments in rats. Methods: On 20 rats, a 15 mm sciatic nerve defect was reconstructed with a nerve autograft. In the Study Group (10 rats), a minced 1 mm nerve segment was seeded around the nerve suture. In the Control Group (10 rats), a nerve graft alone was used. At 4 and 12 weeks, a walking track analysis with open field test (WTA), hystomorphometry (number of myelinated fibers (n), fiber density (FD) and fiber area (FA) and soleus and gastrocnemius muscle weight ratios (MWR) were evaluated. The Student t-test was used for statistical analysis. Results: At 4 and 12 weeks the Study Group had a significantly higher n and FD (p =.043 and.033). The SMWR was significantly higher in the Study Group at 12 weeks (p =.0207). Conclusions: Seeding the distal nerve suture with nerve fragments increases the number of myelinated fibers, the FD and the SMWR. The technique seems promising and deserves further investigation to clarify the mechanisms involved and its functional effects.

UR - http://hdl.handle.net/10447/276461

UR - http://www.tandfonline.com/loi/tacb20#.V6MUQaI_zng

M3 - Article

VL - 118

SP - 27

EP - 35

JO - Default journal

JF - Default journal

ER -